Cargando…
A preliminary study of ALPPS procedure in a rat model
Associating Liver Partition and Portal vein ligation for Staged hepatectomy (ALPPS) has been reported to be a novel surgical technique that provides fast and effective growth of liver remnant. Despite occasional reports on animal studies, the mechanisms of rapid liver regeneration in ALPPS remains u...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668375/ https://www.ncbi.nlm.nih.gov/pubmed/26631552 http://dx.doi.org/10.1038/srep17567 |
_version_ | 1782403980207325184 |
---|---|
author | Shi, Huawen Yang, Guangchao Zheng, Tongsen Wang, Jiabei Li, Lulu Liang, Yingjian Xie, Changming Yin, Dalong Sun, Boshi Sun, Jing Wang, Huanlai Pan, Shangha Jiang, Hongchi Lau, WanYee Liu, Lianxin |
author_facet | Shi, Huawen Yang, Guangchao Zheng, Tongsen Wang, Jiabei Li, Lulu Liang, Yingjian Xie, Changming Yin, Dalong Sun, Boshi Sun, Jing Wang, Huanlai Pan, Shangha Jiang, Hongchi Lau, WanYee Liu, Lianxin |
author_sort | Shi, Huawen |
collection | PubMed |
description | Associating Liver Partition and Portal vein ligation for Staged hepatectomy (ALPPS) has been reported to be a novel surgical technique that provides fast and effective growth of liver remnant. Despite occasional reports on animal studies, the mechanisms of rapid liver regeneration in ALPPS remains unclear. In the present study, we intend to develop a reproducible rat model to mimick ALPPS and to explore the underlying mechanisms. Rats assigned to the portal vein ligation (PVL), left lateral lobe (LLL) resection, transection and sham groups served as controls. Results indicated that the regeneration rate in the remnant liver after ALPPS was two times relative to PVL, whereas rats with transection alone showed minimal volume increase. The expression levels of Ki-67 and PCNA were about ten-fold higher after ALPPS compared with the transection and LLL resection groups, and four-fold higher compared with the PVL group. The levels of TNF-α, IL-6 and HGF in the regenerating liver remnant were about three-fold higher after ALPPS than the controls. There was a more significant activation of NF-κB p65, STAT3 and Yap after ALPPS, suggesting synergistic activation of the pathways by PVL and transection, which might play an important role in liver regeneration after ALPPS. |
format | Online Article Text |
id | pubmed-4668375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46683752015-12-09 A preliminary study of ALPPS procedure in a rat model Shi, Huawen Yang, Guangchao Zheng, Tongsen Wang, Jiabei Li, Lulu Liang, Yingjian Xie, Changming Yin, Dalong Sun, Boshi Sun, Jing Wang, Huanlai Pan, Shangha Jiang, Hongchi Lau, WanYee Liu, Lianxin Sci Rep Article Associating Liver Partition and Portal vein ligation for Staged hepatectomy (ALPPS) has been reported to be a novel surgical technique that provides fast and effective growth of liver remnant. Despite occasional reports on animal studies, the mechanisms of rapid liver regeneration in ALPPS remains unclear. In the present study, we intend to develop a reproducible rat model to mimick ALPPS and to explore the underlying mechanisms. Rats assigned to the portal vein ligation (PVL), left lateral lobe (LLL) resection, transection and sham groups served as controls. Results indicated that the regeneration rate in the remnant liver after ALPPS was two times relative to PVL, whereas rats with transection alone showed minimal volume increase. The expression levels of Ki-67 and PCNA were about ten-fold higher after ALPPS compared with the transection and LLL resection groups, and four-fold higher compared with the PVL group. The levels of TNF-α, IL-6 and HGF in the regenerating liver remnant were about three-fold higher after ALPPS than the controls. There was a more significant activation of NF-κB p65, STAT3 and Yap after ALPPS, suggesting synergistic activation of the pathways by PVL and transection, which might play an important role in liver regeneration after ALPPS. Nature Publishing Group 2015-12-03 /pmc/articles/PMC4668375/ /pubmed/26631552 http://dx.doi.org/10.1038/srep17567 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Shi, Huawen Yang, Guangchao Zheng, Tongsen Wang, Jiabei Li, Lulu Liang, Yingjian Xie, Changming Yin, Dalong Sun, Boshi Sun, Jing Wang, Huanlai Pan, Shangha Jiang, Hongchi Lau, WanYee Liu, Lianxin A preliminary study of ALPPS procedure in a rat model |
title | A preliminary study of ALPPS procedure in a rat model |
title_full | A preliminary study of ALPPS procedure in a rat model |
title_fullStr | A preliminary study of ALPPS procedure in a rat model |
title_full_unstemmed | A preliminary study of ALPPS procedure in a rat model |
title_short | A preliminary study of ALPPS procedure in a rat model |
title_sort | preliminary study of alpps procedure in a rat model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668375/ https://www.ncbi.nlm.nih.gov/pubmed/26631552 http://dx.doi.org/10.1038/srep17567 |
work_keys_str_mv | AT shihuawen apreliminarystudyofalppsprocedureinaratmodel AT yangguangchao apreliminarystudyofalppsprocedureinaratmodel AT zhengtongsen apreliminarystudyofalppsprocedureinaratmodel AT wangjiabei apreliminarystudyofalppsprocedureinaratmodel AT lilulu apreliminarystudyofalppsprocedureinaratmodel AT liangyingjian apreliminarystudyofalppsprocedureinaratmodel AT xiechangming apreliminarystudyofalppsprocedureinaratmodel AT yindalong apreliminarystudyofalppsprocedureinaratmodel AT sunboshi apreliminarystudyofalppsprocedureinaratmodel AT sunjing apreliminarystudyofalppsprocedureinaratmodel AT wanghuanlai apreliminarystudyofalppsprocedureinaratmodel AT panshangha apreliminarystudyofalppsprocedureinaratmodel AT jianghongchi apreliminarystudyofalppsprocedureinaratmodel AT lauwanyee apreliminarystudyofalppsprocedureinaratmodel AT liulianxin apreliminarystudyofalppsprocedureinaratmodel AT shihuawen preliminarystudyofalppsprocedureinaratmodel AT yangguangchao preliminarystudyofalppsprocedureinaratmodel AT zhengtongsen preliminarystudyofalppsprocedureinaratmodel AT wangjiabei preliminarystudyofalppsprocedureinaratmodel AT lilulu preliminarystudyofalppsprocedureinaratmodel AT liangyingjian preliminarystudyofalppsprocedureinaratmodel AT xiechangming preliminarystudyofalppsprocedureinaratmodel AT yindalong preliminarystudyofalppsprocedureinaratmodel AT sunboshi preliminarystudyofalppsprocedureinaratmodel AT sunjing preliminarystudyofalppsprocedureinaratmodel AT wanghuanlai preliminarystudyofalppsprocedureinaratmodel AT panshangha preliminarystudyofalppsprocedureinaratmodel AT jianghongchi preliminarystudyofalppsprocedureinaratmodel AT lauwanyee preliminarystudyofalppsprocedureinaratmodel AT liulianxin preliminarystudyofalppsprocedureinaratmodel |