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Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice
BACKGROUND/OBJECTIVES: High fat diet (HFD) is a major contributor to the development of obesity and cardiovascular diseases due to the induction of cardiac structural and hemodynamic abnormalities. We used a model of diabetic cardiomyopathy in C57Bl/6 mice fed with a HFD to investigate the effects o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668667/ https://www.ncbi.nlm.nih.gov/pubmed/26631050 http://dx.doi.org/10.1186/s12872-015-0154-6 |
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author | Daltro, Pâmela Santana Alves, Paula Santana Castro, Murilo Fagundes Azevedo, Carine M. Vasconcelos, Juliana Fraga Allahdadi, Kyan James de Freitas, Luiz Antônio Rodrigues de Freitas Souza, Bruno Solano dos Santos, Ricardo Ribeiro Soares, Milena Botelho Pereira Macambira, Simone Garcia |
author_facet | Daltro, Pâmela Santana Alves, Paula Santana Castro, Murilo Fagundes Azevedo, Carine M. Vasconcelos, Juliana Fraga Allahdadi, Kyan James de Freitas, Luiz Antônio Rodrigues de Freitas Souza, Bruno Solano dos Santos, Ricardo Ribeiro Soares, Milena Botelho Pereira Macambira, Simone Garcia |
author_sort | Daltro, Pâmela Santana |
collection | PubMed |
description | BACKGROUND/OBJECTIVES: High fat diet (HFD) is a major contributor to the development of obesity and cardiovascular diseases due to the induction of cardiac structural and hemodynamic abnormalities. We used a model of diabetic cardiomyopathy in C57Bl/6 mice fed with a HFD to investigate the effects of granulocyte-colony stimulating factor (G-CSF), a cytokine known for its beneficial effects in the heart, on cardiac anatomical and functional abnormalities associated with obesity and type 2 diabetes. METHODS: Groups of C57Bl/6 mice were fed with standard diet (n = 8) or HFD (n = 16). After 36 weeks, HFD animals were divided into a group treated with G-CSF + standard diet (n = 8) and a vehicle control group + standard diet (n = 8). Cardiac structure and function were assessed by electrocardiography, echocardiography and treadmill tests, in addition to the evaluation of body weight, fasting glicemia, insulin and glucose tolerance at different time points. Histological analyses were performed in the heart tissue. RESULTS: HFD consumption induced metabolic alterations characteristic of type 2 diabetes and obesity, as well as cardiac fibrosis and reduced exercise capacity. Upon returning to a standard diet, obese mice body weight returned to non-obese levels. G-CSF administration accelerated the reduction in of body weight in obese mice. Additionally, G-CSF treatment reduced insulin levels, diminished heart fibrosis, increased exercise capacity and reversed cardiac alterations, including bradycardia, elevated QRS amplitude, augmented P amplitude, increased septal wall thickness, left ventricular posterior thickening and cardiac output reduction. CONCLUSION: Our results indicate that G-CSF administration caused beneficial effects on obesity-associated cardiac impairment. |
format | Online Article Text |
id | pubmed-4668667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46686672015-12-04 Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice Daltro, Pâmela Santana Alves, Paula Santana Castro, Murilo Fagundes Azevedo, Carine M. Vasconcelos, Juliana Fraga Allahdadi, Kyan James de Freitas, Luiz Antônio Rodrigues de Freitas Souza, Bruno Solano dos Santos, Ricardo Ribeiro Soares, Milena Botelho Pereira Macambira, Simone Garcia BMC Cardiovasc Disord Research Article BACKGROUND/OBJECTIVES: High fat diet (HFD) is a major contributor to the development of obesity and cardiovascular diseases due to the induction of cardiac structural and hemodynamic abnormalities. We used a model of diabetic cardiomyopathy in C57Bl/6 mice fed with a HFD to investigate the effects of granulocyte-colony stimulating factor (G-CSF), a cytokine known for its beneficial effects in the heart, on cardiac anatomical and functional abnormalities associated with obesity and type 2 diabetes. METHODS: Groups of C57Bl/6 mice were fed with standard diet (n = 8) or HFD (n = 16). After 36 weeks, HFD animals were divided into a group treated with G-CSF + standard diet (n = 8) and a vehicle control group + standard diet (n = 8). Cardiac structure and function were assessed by electrocardiography, echocardiography and treadmill tests, in addition to the evaluation of body weight, fasting glicemia, insulin and glucose tolerance at different time points. Histological analyses were performed in the heart tissue. RESULTS: HFD consumption induced metabolic alterations characteristic of type 2 diabetes and obesity, as well as cardiac fibrosis and reduced exercise capacity. Upon returning to a standard diet, obese mice body weight returned to non-obese levels. G-CSF administration accelerated the reduction in of body weight in obese mice. Additionally, G-CSF treatment reduced insulin levels, diminished heart fibrosis, increased exercise capacity and reversed cardiac alterations, including bradycardia, elevated QRS amplitude, augmented P amplitude, increased septal wall thickness, left ventricular posterior thickening and cardiac output reduction. CONCLUSION: Our results indicate that G-CSF administration caused beneficial effects on obesity-associated cardiac impairment. BioMed Central 2015-12-03 /pmc/articles/PMC4668667/ /pubmed/26631050 http://dx.doi.org/10.1186/s12872-015-0154-6 Text en © Daltro et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Daltro, Pâmela Santana Alves, Paula Santana Castro, Murilo Fagundes Azevedo, Carine M. Vasconcelos, Juliana Fraga Allahdadi, Kyan James de Freitas, Luiz Antônio Rodrigues de Freitas Souza, Bruno Solano dos Santos, Ricardo Ribeiro Soares, Milena Botelho Pereira Macambira, Simone Garcia Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice |
title | Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice |
title_full | Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice |
title_fullStr | Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice |
title_full_unstemmed | Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice |
title_short | Administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice |
title_sort | administration of granulocyte-colony stimulating factor accompanied with a balanced diet improves cardiac function alterations induced by high fat diet in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668667/ https://www.ncbi.nlm.nih.gov/pubmed/26631050 http://dx.doi.org/10.1186/s12872-015-0154-6 |
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