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RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport

The construction of a large dendritic arbor requires robust growth and the precise delivery of membrane and protein cargoes to specific subcellular regions of the developing dendrite. How the microtubule-based vesicular trafficking and sorting systems are regulated to distribute these dendritic deve...

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Detalles Bibliográficos
Autores principales: Taylor, Caitlin A., Yan, Jing, Howell, Audrey S., Dong, Xintong, Shen, Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669152/
https://www.ncbi.nlm.nih.gov/pubmed/26633194
http://dx.doi.org/10.1371/journal.pgen.1005695
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author Taylor, Caitlin A.
Yan, Jing
Howell, Audrey S.
Dong, Xintong
Shen, Kang
author_facet Taylor, Caitlin A.
Yan, Jing
Howell, Audrey S.
Dong, Xintong
Shen, Kang
author_sort Taylor, Caitlin A.
collection PubMed
description The construction of a large dendritic arbor requires robust growth and the precise delivery of membrane and protein cargoes to specific subcellular regions of the developing dendrite. How the microtubule-based vesicular trafficking and sorting systems are regulated to distribute these dendritic development factors throughout the dendrite is not well understood. Here we identify the small GTPase RAB-10 and the exocyst complex as critical regulators of dendrite morphogenesis and patterning in the C. elegans sensory neuron PVD. In rab-10 mutants, PVD dendritic branches are reduced in the posterior region of the cell but are excessive in the distal anterior region of the cell. We also demonstrate that the dendritic branch distribution within PVD depends on the balance between the molecular motors kinesin-1/UNC-116 and dynein, and we propose that RAB-10 regulates dendrite morphology by balancing the activity of these motors to appropriately distribute branching factors, including the transmembrane receptor DMA-1.
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spelling pubmed-46691522015-12-10 RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport Taylor, Caitlin A. Yan, Jing Howell, Audrey S. Dong, Xintong Shen, Kang PLoS Genet Research Article The construction of a large dendritic arbor requires robust growth and the precise delivery of membrane and protein cargoes to specific subcellular regions of the developing dendrite. How the microtubule-based vesicular trafficking and sorting systems are regulated to distribute these dendritic development factors throughout the dendrite is not well understood. Here we identify the small GTPase RAB-10 and the exocyst complex as critical regulators of dendrite morphogenesis and patterning in the C. elegans sensory neuron PVD. In rab-10 mutants, PVD dendritic branches are reduced in the posterior region of the cell but are excessive in the distal anterior region of the cell. We also demonstrate that the dendritic branch distribution within PVD depends on the balance between the molecular motors kinesin-1/UNC-116 and dynein, and we propose that RAB-10 regulates dendrite morphology by balancing the activity of these motors to appropriately distribute branching factors, including the transmembrane receptor DMA-1. Public Library of Science 2015-12-03 /pmc/articles/PMC4669152/ /pubmed/26633194 http://dx.doi.org/10.1371/journal.pgen.1005695 Text en © 2015 Taylor et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Taylor, Caitlin A.
Yan, Jing
Howell, Audrey S.
Dong, Xintong
Shen, Kang
RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport
title RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport
title_full RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport
title_fullStr RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport
title_full_unstemmed RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport
title_short RAB-10 Regulates Dendritic Branching by Balancing Dendritic Transport
title_sort rab-10 regulates dendritic branching by balancing dendritic transport
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669152/
https://www.ncbi.nlm.nih.gov/pubmed/26633194
http://dx.doi.org/10.1371/journal.pgen.1005695
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