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Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila

In Drosophila, Notch and EGFR signalling pathways are closely intertwined. Their relationship is mostly antagonistic, and may in part be based on the phosphorylation of the Notch signal transducer Suppressor of Hairless [Su(H)] by MAPK. Su(H) is a transcription factor that together with several cofa...

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Autores principales: Auer, Jasmin S., Nagel, Anja C., Schulz, Adriana, Wahl, Vanessa, Preiss, Anette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669530/
https://www.ncbi.nlm.nih.gov/pubmed/26702412
http://dx.doi.org/10.1016/j.dib.2015.11.004
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author Auer, Jasmin S.
Nagel, Anja C.
Schulz, Adriana
Wahl, Vanessa
Preiss, Anette
author_facet Auer, Jasmin S.
Nagel, Anja C.
Schulz, Adriana
Wahl, Vanessa
Preiss, Anette
author_sort Auer, Jasmin S.
collection PubMed
description In Drosophila, Notch and EGFR signalling pathways are closely intertwined. Their relationship is mostly antagonistic, and may in part be based on the phosphorylation of the Notch signal transducer Suppressor of Hairless [Su(H)] by MAPK. Su(H) is a transcription factor that together with several cofactors regulates the expression of Notch target genes. Here we address the consequences of a local induction of three Su(H) variants on Notch target gene expression. To this end, wild-type Su(H), a phospho-deficient Su(H)(MAPK-)(ko) and a phospho-mimetic Su(H)(MAPK-ac) isoform were overexpressed in the central domain of the wing anlagen. The expression of the Notch target genes cut, wingless, E(spl)m8-HLH and vestigial, was monitored. For the latter two, reporter genes were used (E(spl)m8-lacZ, vg(BE)-lacZ). In general, Su(H)(MAPK-)(ko) induced a stronger response than wild-type Su(H), whereas the response to Su(H)(MAPK-ac) was very weak. Notch target genes cut, wingless and vg(BE)-lacZ were ectopically activated, whereas E(spl)m8-lacZ was repressed by overexpression of Su(H) proteins. In addition, in epistasis experiments an activated form of the EGF-receptor (DER(act)) or the MAPK (rl(SEM)) and individual Su(H) variants were co-overexpressed locally, to compare the resultant phenotypes in adult flies (thorax, wings and eyes) as well as to assay the response of the Notch target gene cut in cell clones.
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spelling pubmed-46695302015-12-23 Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila Auer, Jasmin S. Nagel, Anja C. Schulz, Adriana Wahl, Vanessa Preiss, Anette Data Brief Data Article In Drosophila, Notch and EGFR signalling pathways are closely intertwined. Their relationship is mostly antagonistic, and may in part be based on the phosphorylation of the Notch signal transducer Suppressor of Hairless [Su(H)] by MAPK. Su(H) is a transcription factor that together with several cofactors regulates the expression of Notch target genes. Here we address the consequences of a local induction of three Su(H) variants on Notch target gene expression. To this end, wild-type Su(H), a phospho-deficient Su(H)(MAPK-)(ko) and a phospho-mimetic Su(H)(MAPK-ac) isoform were overexpressed in the central domain of the wing anlagen. The expression of the Notch target genes cut, wingless, E(spl)m8-HLH and vestigial, was monitored. For the latter two, reporter genes were used (E(spl)m8-lacZ, vg(BE)-lacZ). In general, Su(H)(MAPK-)(ko) induced a stronger response than wild-type Su(H), whereas the response to Su(H)(MAPK-ac) was very weak. Notch target genes cut, wingless and vg(BE)-lacZ were ectopically activated, whereas E(spl)m8-lacZ was repressed by overexpression of Su(H) proteins. In addition, in epistasis experiments an activated form of the EGF-receptor (DER(act)) or the MAPK (rl(SEM)) and individual Su(H) variants were co-overexpressed locally, to compare the resultant phenotypes in adult flies (thorax, wings and eyes) as well as to assay the response of the Notch target gene cut in cell clones. Elsevier 2015-11-10 /pmc/articles/PMC4669530/ /pubmed/26702412 http://dx.doi.org/10.1016/j.dib.2015.11.004 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Auer, Jasmin S.
Nagel, Anja C.
Schulz, Adriana
Wahl, Vanessa
Preiss, Anette
Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila
title Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila
title_full Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila
title_fullStr Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila
title_full_unstemmed Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila
title_short Local overexpression of Su(H)-MAPK variants affects Notch target gene expression and adult phenotypes in Drosophila
title_sort local overexpression of su(h)-mapk variants affects notch target gene expression and adult phenotypes in drosophila
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669530/
https://www.ncbi.nlm.nih.gov/pubmed/26702412
http://dx.doi.org/10.1016/j.dib.2015.11.004
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