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Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis

The recent widespread applications of nanomaterials, because of their properties, opens new scenarios that affect their dispersal in the environment. In particular multiwall carbon nanotubes (MWCNTs), despite their qualities, seem to be harmful for animals and humans. To evaluate possible toxic effe...

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Autores principales: Girardello, Rossana, Tasselli, Stefano, Baranzini, Nicolò, Valvassori, Roberto, de Eguileor, Magda, Grimaldi, Annalisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670124/
https://www.ncbi.nlm.nih.gov/pubmed/26636582
http://dx.doi.org/10.1371/journal.pone.0144361
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author Girardello, Rossana
Tasselli, Stefano
Baranzini, Nicolò
Valvassori, Roberto
de Eguileor, Magda
Grimaldi, Annalisa
author_facet Girardello, Rossana
Tasselli, Stefano
Baranzini, Nicolò
Valvassori, Roberto
de Eguileor, Magda
Grimaldi, Annalisa
author_sort Girardello, Rossana
collection PubMed
description The recent widespread applications of nanomaterials, because of their properties, opens new scenarios that affect their dispersal in the environment. In particular multiwall carbon nanotubes (MWCNTs), despite their qualities, seem to be harmful for animals and humans. To evaluate possible toxic effects caused by carbon nanotube environmental dispersion, with regard to aquatic compartment, we proposed as experimental model a freshwater invertebrate: Hirudo medicinalis. In the present study we analyse acute and chronic immune responses over a short (1, 3, 6 and 12 hours) and long time (from 1 to 5 weeks) exposure to MWCNTs by optical, electron and immunohistochemical approaches. In the exposed leeches angiogenesis and fibroplasia accompanied by massive cellular migration occur. Immunocytochemical characterization using specific markers shows that in these inflammatory processes the monocyte-macrophages (CD45(+), CD68(+)) are the most involved cells. These immunocompetent cells are characterized by sequence of events starting from the expression of pro-inflammatory cytokines (in particular IL-18), and amyloidogenensis. Our combined experimental approaches, basing on high sensitive inflammatory response can highlight adverse effects of nanomaterials on aquatic organisms and could be useful to assess the MWCNTs impact on aquatic, terrestrial animal and human health.
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spelling pubmed-46701242015-12-10 Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis Girardello, Rossana Tasselli, Stefano Baranzini, Nicolò Valvassori, Roberto de Eguileor, Magda Grimaldi, Annalisa PLoS One Research Article The recent widespread applications of nanomaterials, because of their properties, opens new scenarios that affect their dispersal in the environment. In particular multiwall carbon nanotubes (MWCNTs), despite their qualities, seem to be harmful for animals and humans. To evaluate possible toxic effects caused by carbon nanotube environmental dispersion, with regard to aquatic compartment, we proposed as experimental model a freshwater invertebrate: Hirudo medicinalis. In the present study we analyse acute and chronic immune responses over a short (1, 3, 6 and 12 hours) and long time (from 1 to 5 weeks) exposure to MWCNTs by optical, electron and immunohistochemical approaches. In the exposed leeches angiogenesis and fibroplasia accompanied by massive cellular migration occur. Immunocytochemical characterization using specific markers shows that in these inflammatory processes the monocyte-macrophages (CD45(+), CD68(+)) are the most involved cells. These immunocompetent cells are characterized by sequence of events starting from the expression of pro-inflammatory cytokines (in particular IL-18), and amyloidogenensis. Our combined experimental approaches, basing on high sensitive inflammatory response can highlight adverse effects of nanomaterials on aquatic organisms and could be useful to assess the MWCNTs impact on aquatic, terrestrial animal and human health. Public Library of Science 2015-12-04 /pmc/articles/PMC4670124/ /pubmed/26636582 http://dx.doi.org/10.1371/journal.pone.0144361 Text en © 2015 Girardello et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Girardello, Rossana
Tasselli, Stefano
Baranzini, Nicolò
Valvassori, Roberto
de Eguileor, Magda
Grimaldi, Annalisa
Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis
title Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis
title_full Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis
title_fullStr Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis
title_full_unstemmed Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis
title_short Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis
title_sort effects of carbon nanotube environmental dispersion on an aquatic invertebrate, hirudo medicinalis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670124/
https://www.ncbi.nlm.nih.gov/pubmed/26636582
http://dx.doi.org/10.1371/journal.pone.0144361
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