“In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain

BACKGROUND AND OBJECTIVES: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccina...

Descripción completa

Detalles Bibliográficos
Autores principales: Sekhavati, Mohammad, Tadayon, Keyvan, Ghaderi, Rainak, Banihashemi, Reza, Jabbari, Ahmad Reza, Shokri, Gholamreza, Karimnasab, Nasim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670467/
https://www.ncbi.nlm.nih.gov/pubmed/26644873
_version_ 1782404259356082176
author Sekhavati, Mohammad
Tadayon, Keyvan
Ghaderi, Rainak
Banihashemi, Reza
Jabbari, Ahmad Reza
Shokri, Gholamreza
Karimnasab, Nasim
author_facet Sekhavati, Mohammad
Tadayon, Keyvan
Ghaderi, Rainak
Banihashemi, Reza
Jabbari, Ahmad Reza
Shokri, Gholamreza
Karimnasab, Nasim
author_sort Sekhavati, Mohammad
collection PubMed
description BACKGROUND AND OBJECTIVES: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker. RESULTS AND CONCLUSION: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.
format Online
Article
Text
id pubmed-4670467
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Tehran University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-46704672015-12-07 “In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain Sekhavati, Mohammad Tadayon, Keyvan Ghaderi, Rainak Banihashemi, Reza Jabbari, Ahmad Reza Shokri, Gholamreza Karimnasab, Nasim Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker. RESULTS AND CONCLUSION: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding. Tehran University of Medical Sciences 2015-02 /pmc/articles/PMC4670467/ /pubmed/26644873 Text en © Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Sekhavati, Mohammad
Tadayon, Keyvan
Ghaderi, Rainak
Banihashemi, Reza
Jabbari, Ahmad Reza
Shokri, Gholamreza
Karimnasab, Nasim
“In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
title “In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
title_full “In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
title_fullStr “In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
title_full_unstemmed “In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
title_short “In-house” production of DNA size marker from a vaccinal Bacillus anthracis strain
title_sort “in-house” production of dna size marker from a vaccinal bacillus anthracis strain
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670467/
https://www.ncbi.nlm.nih.gov/pubmed/26644873
work_keys_str_mv AT sekhavatimohammad inhouseproductionofdnasizemarkerfromavaccinalbacillusanthracisstrain
AT tadayonkeyvan inhouseproductionofdnasizemarkerfromavaccinalbacillusanthracisstrain
AT ghaderirainak inhouseproductionofdnasizemarkerfromavaccinalbacillusanthracisstrain
AT banihashemireza inhouseproductionofdnasizemarkerfromavaccinalbacillusanthracisstrain
AT jabbariahmadreza inhouseproductionofdnasizemarkerfromavaccinalbacillusanthracisstrain
AT shokrigholamreza inhouseproductionofdnasizemarkerfromavaccinalbacillusanthracisstrain
AT karimnasabnasim inhouseproductionofdnasizemarkerfromavaccinalbacillusanthracisstrain

Ejemplares similares