Removal of redundant contigs from de novo RNA-Seq assemblies via homology search improves accurate detection of differentially expressed genes

BACKGROUND: For plant species with unsequenced genomes, cDNA contigs created by de novo assembly of RNA-Seq reads are used as reference sequences for comparative analysis of RNA-Seq datasets and the detection of differentially expressed genes (DEGs). Redundancies in such contigs are evident in previ...

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Detalles Bibliográficos
Autores principales: Ono, Hanako, Ishii, Kazuo, Kozaki, Toshinori, Ogiwara, Isao, Kanekatsu, Motoki, Yamada, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670531/
https://www.ncbi.nlm.nih.gov/pubmed/26637306
http://dx.doi.org/10.1186/s12864-015-2247-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670531/
https://www.ncbi.nlm.nih.gov/pubmed/26637306
http://dx.doi.org/10.1186/s12864-015-2247-0

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