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Removal of redundant contigs from de novo RNA-Seq assemblies via homology search improves accurate detection of differentially expressed genes
BACKGROUND: For plant species with unsequenced genomes, cDNA contigs created by de novo assembly of RNA-Seq reads are used as reference sequences for comparative analysis of RNA-Seq datasets and the detection of differentially expressed genes (DEGs). Redundancies in such contigs are evident in previ...
Autores principales: | Ono, Hanako, Ishii, Kazuo, Kozaki, Toshinori, Ogiwara, Isao, Kanekatsu, Motoki, Yamada, Tetsuya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4670531/ https://www.ncbi.nlm.nih.gov/pubmed/26637306 http://dx.doi.org/10.1186/s12864-015-2247-0 |
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