Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin

Advancing the practical utility of nonlinear optical microscopy requires continued improvement in imaging depth and contrast. We evaluated second‐harmonic generation (SHG) and third‐harmonic generation images from ex vivo human skin and showed that a sub‐40 fs, 1060‐nm Yb‐fiber laser can enhance SHG...

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Detalles Bibliográficos
Autores principales: Balu, Mihaela, Saytashev, Ilyas, Hou, Jue, Dantus, Marcos, Tromberg, Bruce J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo‐Optical Instrumentation Engineers 2015
Materias:
JBO Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671301/
https://www.ncbi.nlm.nih.gov/pubmed/26641198
http://dx.doi.org/10.1117/1.JBO.20.12.120501
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author Balu, Mihaela
Saytashev, Ilyas
Hou, Jue
Dantus, Marcos
Tromberg, Bruce J.
author_facet Balu, Mihaela
Saytashev, Ilyas
Hou, Jue
Dantus, Marcos
Tromberg, Bruce J.
author_sort Balu, Mihaela
collection PubMed
description Advancing the practical utility of nonlinear optical microscopy requires continued improvement in imaging depth and contrast. We evaluated second‐harmonic generation (SHG) and third‐harmonic generation images from ex vivo human skin and showed that a sub‐40 fs, 1060‐nm Yb‐fiber laser can enhance SHG penetration depth by up to 80% compared to a [Formula: see text] , 800 nm Ti:sapphire source. These results demonstrate the potential of fiber‐based laser systems to address a key performance limitation related to nonlinear optical microscopy (NLOM) technology while providing a low‐barrier‐to‐access alternative to Ti:sapphire sources that could help accelerate the movement of NLOM into clinical practice.
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spelling pubmed-46713012016-12-07 Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin Balu, Mihaela Saytashev, Ilyas Hou, Jue Dantus, Marcos Tromberg, Bruce J. J Biomed Opt JBO Letters Advancing the practical utility of nonlinear optical microscopy requires continued improvement in imaging depth and contrast. We evaluated second‐harmonic generation (SHG) and third‐harmonic generation images from ex vivo human skin and showed that a sub‐40 fs, 1060‐nm Yb‐fiber laser can enhance SHG penetration depth by up to 80% compared to a [Formula: see text] , 800 nm Ti:sapphire source. These results demonstrate the potential of fiber‐based laser systems to address a key performance limitation related to nonlinear optical microscopy (NLOM) technology while providing a low‐barrier‐to‐access alternative to Ti:sapphire sources that could help accelerate the movement of NLOM into clinical practice. Society of Photo‐Optical Instrumentation Engineers 2015-12-07 2015-12 /pmc/articles/PMC4671301/ /pubmed/26641198 http://dx.doi.org/10.1117/1.JBO.20.12.120501 Text en © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle JBO Letters
Balu, Mihaela
Saytashev, Ilyas
Hou, Jue
Dantus, Marcos
Tromberg, Bruce J.
Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin
title Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin
title_full Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin
title_fullStr Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin
title_full_unstemmed Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin
title_short Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin
title_sort sub‐40 fs, 1060‐nm yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin
topic JBO Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671301/
https://www.ncbi.nlm.nih.gov/pubmed/26641198
http://dx.doi.org/10.1117/1.JBO.20.12.120501
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