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In Vitro Fertilization, Levels of Pro-Inflammatory Factors and Lipid Peroxidation
BACKGROUND: Infertility is a problem concerning 10-15% of the individuals in the fertile period. This study investigated effects of proinflammatory factors as well as lipid hydroperoxides (LPO) levels upon in vitro fertilization (IVF) success. MATERIALS AND METHODS: In this prospective, non-randomiz...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671383/ https://www.ncbi.nlm.nih.gov/pubmed/26644849 |
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author | Yildizfer, Ferdag Donma, Orkide Yen, Mustafa Ekmekci, Ozlem Karatas Kul, Zubeyde Asli Keser, Zafer Esat Imal, Ahmet Cagil, Emin Mengi, Murat Ekmekci, Hakan Sahmay, Sezai Nalbantoglu, Burcin Donma, Mustafa Metin |
author_facet | Yildizfer, Ferdag Donma, Orkide Yen, Mustafa Ekmekci, Ozlem Karatas Kul, Zubeyde Asli Keser, Zafer Esat Imal, Ahmet Cagil, Emin Mengi, Murat Ekmekci, Hakan Sahmay, Sezai Nalbantoglu, Burcin Donma, Mustafa Metin |
author_sort | Yildizfer, Ferdag |
collection | PubMed |
description | BACKGROUND: Infertility is a problem concerning 10-15% of the individuals in the fertile period. This study investigated effects of proinflammatory factors as well as lipid hydroperoxides (LPO) levels upon in vitro fertilization (IVF) success. MATERIALS AND METHODS: In this prospective, non-randomized, controlled clinical study, sera obtained from 26 fertile (group-1), 26 infertile women before (group-2) and after (group-3) IVF treatment were analyzed. Leptin, leptin receptor, resistin, tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) were analyzed using enzyme-linked immunosorbent assay (ELISA). LPO was determined spectrophotometrically. Mann- Whitney U test, paired samples t test, Wilcoxon signed-rank test as well as Pearson correlation analysis by SPSS were performed for statistical analysis. RESULTS: TNF-α, resistin and LPO levels increased (P=0.020, P=0.003, P=0.001, respectively) in group-3 compared to group-2. A significant increase in LPO was noted both in group-2 and -3 compared to controls (P=0.000). LPO were higher in non-pregnants than pregnants in group-2. For pregnants, significant correlations were observed between leptin and resistin in group-2 and TNF-α and leptin in group-3. None of these correlations were found for the women, who could not conceive. CONCLUSION: LPO, leptin-resistin correlation, associations with TNF-α may be helpful during the interpretation of IVF success rates. |
format | Online Article Text |
id | pubmed-4671383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-46713832015-12-07 In Vitro Fertilization, Levels of Pro-Inflammatory Factors and Lipid Peroxidation Yildizfer, Ferdag Donma, Orkide Yen, Mustafa Ekmekci, Ozlem Karatas Kul, Zubeyde Asli Keser, Zafer Esat Imal, Ahmet Cagil, Emin Mengi, Murat Ekmekci, Hakan Sahmay, Sezai Nalbantoglu, Burcin Donma, Mustafa Metin Int J Fertil Steril Original Article BACKGROUND: Infertility is a problem concerning 10-15% of the individuals in the fertile period. This study investigated effects of proinflammatory factors as well as lipid hydroperoxides (LPO) levels upon in vitro fertilization (IVF) success. MATERIALS AND METHODS: In this prospective, non-randomized, controlled clinical study, sera obtained from 26 fertile (group-1), 26 infertile women before (group-2) and after (group-3) IVF treatment were analyzed. Leptin, leptin receptor, resistin, tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) were analyzed using enzyme-linked immunosorbent assay (ELISA). LPO was determined spectrophotometrically. Mann- Whitney U test, paired samples t test, Wilcoxon signed-rank test as well as Pearson correlation analysis by SPSS were performed for statistical analysis. RESULTS: TNF-α, resistin and LPO levels increased (P=0.020, P=0.003, P=0.001, respectively) in group-3 compared to group-2. A significant increase in LPO was noted both in group-2 and -3 compared to controls (P=0.000). LPO were higher in non-pregnants than pregnants in group-2. For pregnants, significant correlations were observed between leptin and resistin in group-2 and TNF-α and leptin in group-3. None of these correlations were found for the women, who could not conceive. CONCLUSION: LPO, leptin-resistin correlation, associations with TNF-α may be helpful during the interpretation of IVF success rates. Royan Institute 2015 2015-10-31 /pmc/articles/PMC4671383/ /pubmed/26644849 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yildizfer, Ferdag Donma, Orkide Yen, Mustafa Ekmekci, Ozlem Karatas Kul, Zubeyde Asli Keser, Zafer Esat Imal, Ahmet Cagil, Emin Mengi, Murat Ekmekci, Hakan Sahmay, Sezai Nalbantoglu, Burcin Donma, Mustafa Metin In Vitro Fertilization, Levels of Pro-Inflammatory Factors and Lipid Peroxidation |
title | In Vitro Fertilization, Levels of Pro-Inflammatory
Factors and Lipid Peroxidation |
title_full | In Vitro Fertilization, Levels of Pro-Inflammatory
Factors and Lipid Peroxidation |
title_fullStr | In Vitro Fertilization, Levels of Pro-Inflammatory
Factors and Lipid Peroxidation |
title_full_unstemmed | In Vitro Fertilization, Levels of Pro-Inflammatory
Factors and Lipid Peroxidation |
title_short | In Vitro Fertilization, Levels of Pro-Inflammatory
Factors and Lipid Peroxidation |
title_sort | in vitro fertilization, levels of pro-inflammatory
factors and lipid peroxidation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671383/ https://www.ncbi.nlm.nih.gov/pubmed/26644849 |
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