Cargando…

Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis

We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, aden...

Descripción completa

Detalles Bibliográficos
Autores principales: de Sá, V.K., Rocha, T.P., Moreira, AL., Soares, F.A., Takagaki, T., Carvalho, L., Nicholson, A.G., Capelozzi, V.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671531/
https://www.ncbi.nlm.nih.gov/pubmed/26352698
http://dx.doi.org/10.1590/1414-431X20154693
_version_ 1782404409034014720
author de Sá, V.K.
Rocha, T.P.
Moreira, AL.
Soares, F.A.
Takagaki, T.
Carvalho, L.
Nicholson, A.G.
Capelozzi, V.L.
author_facet de Sá, V.K.
Rocha, T.P.
Moreira, AL.
Soares, F.A.
Takagaki, T.
Carvalho, L.
Nicholson, A.G.
Capelozzi, V.L.
author_sort de Sá, V.K.
collection PubMed
description We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous hyperplasia (P=0.0001), or severe dysplasia (P=0.03). Lower expression of Hyal-3 was found in atypical adenomatous hyperplasia than in basal cell hyperplasia (P=0.01) or moderate dysplasia (P=0.02). HAS-2 was significantly higher in severe dysplasia (P=0.002) and in squamous metaplasia (P=0.04) compared with basal cell hyperplasia. HAS-3 was significantly expressed in basal cell hyperplasia compared with atypical adenomatous hyperplasia (P=0.05) and severe dysplasia (P=0.02). Lower expression of HAS-3 was found in severe dysplasia compared with squamous metaplasia (P=0.01) and moderate dysplasia (P=0.01). Epithelial Hyal-1 and −3 and HAS-1, −2, and −3 expressions were significantly higher in pre-neoplastic lesions than in neoplastic lesions. Comparative Cox multivariate analysis controlled by N stage and histologic tumor type showed that patients with high HAS-3 expression in pre-neoplastic cells obtained by lung/bronchial biopsy presented a significantly higher risk of death (HR=1.19; P=0.04). We concluded that localization of Hyal and HAS in lung/bronchial pre-neoplastic and neoplastic lesions was inversely related to malignancy, which implied that visualizing these factors could be a useful diagnostic procedure for suspected lung cancer. Finalizing this conclusion will require a wider study in a randomized and prospective trial.
format Online
Article
Text
id pubmed-4671531
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Associação Brasileira de Divulgação Científica
record_format MEDLINE/PubMed
spelling pubmed-46715312015-12-22 Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis de Sá, V.K. Rocha, T.P. Moreira, AL. Soares, F.A. Takagaki, T. Carvalho, L. Nicholson, A.G. Capelozzi, V.L. Braz J Med Biol Res Clinical Investigation We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous hyperplasia (P=0.0001), or severe dysplasia (P=0.03). Lower expression of Hyal-3 was found in atypical adenomatous hyperplasia than in basal cell hyperplasia (P=0.01) or moderate dysplasia (P=0.02). HAS-2 was significantly higher in severe dysplasia (P=0.002) and in squamous metaplasia (P=0.04) compared with basal cell hyperplasia. HAS-3 was significantly expressed in basal cell hyperplasia compared with atypical adenomatous hyperplasia (P=0.05) and severe dysplasia (P=0.02). Lower expression of HAS-3 was found in severe dysplasia compared with squamous metaplasia (P=0.01) and moderate dysplasia (P=0.01). Epithelial Hyal-1 and −3 and HAS-1, −2, and −3 expressions were significantly higher in pre-neoplastic lesions than in neoplastic lesions. Comparative Cox multivariate analysis controlled by N stage and histologic tumor type showed that patients with high HAS-3 expression in pre-neoplastic cells obtained by lung/bronchial biopsy presented a significantly higher risk of death (HR=1.19; P=0.04). We concluded that localization of Hyal and HAS in lung/bronchial pre-neoplastic and neoplastic lesions was inversely related to malignancy, which implied that visualizing these factors could be a useful diagnostic procedure for suspected lung cancer. Finalizing this conclusion will require a wider study in a randomized and prospective trial. Associação Brasileira de Divulgação Científica 2015-08-28 /pmc/articles/PMC4671531/ /pubmed/26352698 http://dx.doi.org/10.1590/1414-431X20154693 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
spellingShingle Clinical Investigation
de Sá, V.K.
Rocha, T.P.
Moreira, AL.
Soares, F.A.
Takagaki, T.
Carvalho, L.
Nicholson, A.G.
Capelozzi, V.L.
Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
title Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
title_full Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
title_fullStr Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
title_full_unstemmed Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
title_short Hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
title_sort hyaluronidases and hyaluronan synthases expression is inversely correlated with malignancy in lung/bronchial pre-neoplastic and neoplastic lesions, affecting prognosis
topic Clinical Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671531/
https://www.ncbi.nlm.nih.gov/pubmed/26352698
http://dx.doi.org/10.1590/1414-431X20154693
work_keys_str_mv AT desavk hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis
AT rochatp hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis
AT moreiraal hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis
AT soaresfa hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis
AT takagakit hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis
AT carvalhol hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis
AT nicholsonag hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis
AT capelozzivl hyaluronidasesandhyaluronansynthasesexpressionisinverselycorrelatedwithmalignancyinlungbronchialpreneoplasticandneoplasticlesionsaffectingprognosis