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Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling
Microbial polysaccharides represent an important class of microbial polymers with diverse functions such as biofilm formation, thickening, and gelling properties as well as health-promoting properties. The broad range of exopolysaccharide (EPS) functionalities has sparked a renewed interest in this...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672062/ https://www.ncbi.nlm.nih.gov/pubmed/26696983 http://dx.doi.org/10.3389/fmicb.2015.01374 |
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author | Larsen, Flemming H. Engelsen, Søren B. |
author_facet | Larsen, Flemming H. Engelsen, Søren B. |
author_sort | Larsen, Flemming H. |
collection | PubMed |
description | Microbial polysaccharides represent an important class of microbial polymers with diverse functions such as biofilm formation, thickening, and gelling properties as well as health-promoting properties. The broad range of exopolysaccharide (EPS) functionalities has sparked a renewed interest in this class of molecules. Chemical, enzymatic as well as genetic modifications by metabolic engineering can be used to create large numbers of analogous EPS variants with respect to EPS functionality. While this top–down approach is effective in finding new candidates for desired functionality, there seems to be a lack of the corresponding bottom–up approach. The molecular mechanisms of the desired functionalities can be established from Nuclear Magnetic Resonance (NMR) and molecular models and it is proposed that these models can be fed back into the biotechnology by using a quantitative structure–property approach. In this way it will be possible to tailor specific functionality within a given design space. This perspective will include two well-known commercial microbial EPS examples namely gellan and diutan and show how even a limited use of multiphase NMR and molecular modeling can increase the insight into their different properties, which are based on only minor structural differences. |
format | Online Article Text |
id | pubmed-4672062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46720622015-12-22 Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling Larsen, Flemming H. Engelsen, Søren B. Front Microbiol Microbiology Microbial polysaccharides represent an important class of microbial polymers with diverse functions such as biofilm formation, thickening, and gelling properties as well as health-promoting properties. The broad range of exopolysaccharide (EPS) functionalities has sparked a renewed interest in this class of molecules. Chemical, enzymatic as well as genetic modifications by metabolic engineering can be used to create large numbers of analogous EPS variants with respect to EPS functionality. While this top–down approach is effective in finding new candidates for desired functionality, there seems to be a lack of the corresponding bottom–up approach. The molecular mechanisms of the desired functionalities can be established from Nuclear Magnetic Resonance (NMR) and molecular models and it is proposed that these models can be fed back into the biotechnology by using a quantitative structure–property approach. In this way it will be possible to tailor specific functionality within a given design space. This perspective will include two well-known commercial microbial EPS examples namely gellan and diutan and show how even a limited use of multiphase NMR and molecular modeling can increase the insight into their different properties, which are based on only minor structural differences. Frontiers Media S.A. 2015-12-08 /pmc/articles/PMC4672062/ /pubmed/26696983 http://dx.doi.org/10.3389/fmicb.2015.01374 Text en Copyright © 2015 Larsen and Engelsen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Larsen, Flemming H. Engelsen, Søren B. Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling |
title | Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling |
title_full | Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling |
title_fullStr | Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling |
title_full_unstemmed | Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling |
title_short | Insight into the Functionality of Microbial Exopolysaccharides by NMR Spectroscopy and Molecular Modeling |
title_sort | insight into the functionality of microbial exopolysaccharides by nmr spectroscopy and molecular modeling |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672062/ https://www.ncbi.nlm.nih.gov/pubmed/26696983 http://dx.doi.org/10.3389/fmicb.2015.01374 |
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