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Purification, Characterization of L-Methioninase from Candida tropicalis, and Its Application as an Anticancer
The aim of the present study is to purify L-methioninase from Candida tropicalis 34.19-fold with 27.98% recovery after ion exchange chromatography followed by gel filtration. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular weight of 46 KDa. Its optimum temperature was 45...
Autores principales: | Selim, Mohsen Helmy, Karm Eldin, El-Zahraa, Saad, Moataza Mahmoud, Mostafa, El-Sayed Eliwa, Shetia, Yosrea Hassan, Anise, Amany Ahmed Hassabo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672112/ https://www.ncbi.nlm.nih.gov/pubmed/26691554 http://dx.doi.org/10.1155/2015/173140 |
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