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3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medic...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672115/ https://www.ncbi.nlm.nih.gov/pubmed/26688810 http://dx.doi.org/10.1155/2015/432645 |
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author | Wu, Di Wan, Jian Huang, Yan Guo, Yibing Xu, Tianxin Zhu, Mingyan Fan, Xiangjun Zhu, Shajun Ling, Changchun Li, Xiaohong Lu, Jingjing Zhu, Hui Zhou, Pengcheng Lu, Yuhua Wang, Zhiwei |
author_facet | Wu, Di Wan, Jian Huang, Yan Guo, Yibing Xu, Tianxin Zhu, Mingyan Fan, Xiangjun Zhu, Shajun Ling, Changchun Li, Xiaohong Lu, Jingjing Zhu, Hui Zhou, Pengcheng Lu, Yuhua Wang, Zhiwei |
author_sort | Wu, Di |
collection | PubMed |
description | Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6 β cells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients. |
format | Online Article Text |
id | pubmed-4672115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46721152015-12-20 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study Wu, Di Wan, Jian Huang, Yan Guo, Yibing Xu, Tianxin Zhu, Mingyan Fan, Xiangjun Zhu, Shajun Ling, Changchun Li, Xiaohong Lu, Jingjing Zhu, Hui Zhou, Pengcheng Lu, Yuhua Wang, Zhiwei Biomed Res Int Research Article Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6 β cells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients. Hindawi Publishing Corporation 2015 2015-11-24 /pmc/articles/PMC4672115/ /pubmed/26688810 http://dx.doi.org/10.1155/2015/432645 Text en Copyright © 2015 Di Wu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wu, Di Wan, Jian Huang, Yan Guo, Yibing Xu, Tianxin Zhu, Mingyan Fan, Xiangjun Zhu, Shajun Ling, Changchun Li, Xiaohong Lu, Jingjing Zhu, Hui Zhou, Pengcheng Lu, Yuhua Wang, Zhiwei 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title | 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_full | 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_fullStr | 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_full_unstemmed | 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_short | 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study |
title_sort | 3d culture of min-6 cells on decellularized pancreatic scaffold: in vitro and in vivo study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672115/ https://www.ncbi.nlm.nih.gov/pubmed/26688810 http://dx.doi.org/10.1155/2015/432645 |
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