Cargando…

3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study

Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medic...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Di, Wan, Jian, Huang, Yan, Guo, Yibing, Xu, Tianxin, Zhu, Mingyan, Fan, Xiangjun, Zhu, Shajun, Ling, Changchun, Li, Xiaohong, Lu, Jingjing, Zhu, Hui, Zhou, Pengcheng, Lu, Yuhua, Wang, Zhiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672115/
https://www.ncbi.nlm.nih.gov/pubmed/26688810
http://dx.doi.org/10.1155/2015/432645
_version_ 1782404507123056640
author Wu, Di
Wan, Jian
Huang, Yan
Guo, Yibing
Xu, Tianxin
Zhu, Mingyan
Fan, Xiangjun
Zhu, Shajun
Ling, Changchun
Li, Xiaohong
Lu, Jingjing
Zhu, Hui
Zhou, Pengcheng
Lu, Yuhua
Wang, Zhiwei
author_facet Wu, Di
Wan, Jian
Huang, Yan
Guo, Yibing
Xu, Tianxin
Zhu, Mingyan
Fan, Xiangjun
Zhu, Shajun
Ling, Changchun
Li, Xiaohong
Lu, Jingjing
Zhu, Hui
Zhou, Pengcheng
Lu, Yuhua
Wang, Zhiwei
author_sort Wu, Di
collection PubMed
description Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6 β cells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients.
format Online
Article
Text
id pubmed-4672115
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-46721152015-12-20 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study Wu, Di Wan, Jian Huang, Yan Guo, Yibing Xu, Tianxin Zhu, Mingyan Fan, Xiangjun Zhu, Shajun Ling, Changchun Li, Xiaohong Lu, Jingjing Zhu, Hui Zhou, Pengcheng Lu, Yuhua Wang, Zhiwei Biomed Res Int Research Article Type 1 diabetes is an autoimmune disease which is due to the lack of β cells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6 β cells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients. Hindawi Publishing Corporation 2015 2015-11-24 /pmc/articles/PMC4672115/ /pubmed/26688810 http://dx.doi.org/10.1155/2015/432645 Text en Copyright © 2015 Di Wu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wu, Di
Wan, Jian
Huang, Yan
Guo, Yibing
Xu, Tianxin
Zhu, Mingyan
Fan, Xiangjun
Zhu, Shajun
Ling, Changchun
Li, Xiaohong
Lu, Jingjing
Zhu, Hui
Zhou, Pengcheng
Lu, Yuhua
Wang, Zhiwei
3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
title 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
title_full 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
title_fullStr 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
title_full_unstemmed 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
title_short 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
title_sort 3d culture of min-6 cells on decellularized pancreatic scaffold: in vitro and in vivo study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672115/
https://www.ncbi.nlm.nih.gov/pubmed/26688810
http://dx.doi.org/10.1155/2015/432645
work_keys_str_mv AT wudi 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT wanjian 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT huangyan 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT guoyibing 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT xutianxin 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT zhumingyan 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT fanxiangjun 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT zhushajun 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT lingchangchun 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT lixiaohong 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT lujingjing 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT zhuhui 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT zhoupengcheng 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT luyuhua 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy
AT wangzhiwei 3dcultureofmin6cellsondecellularizedpancreaticscaffoldinvitroandinvivostudy