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Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides

To date, studies on host preferences and blood meal identification have been conducted for Culicoides species using molecular-based methods such as PCR techniques to amplify only a fragment from universal vertebrate mitochondrial genes such as cytochrome c oxidase subunit I or cytochrome b (Cyt b)....

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Autores principales: Hadj-Henni, Leila, De Meulemeester, Thibaut, Depaquit, Jérôme, Noël, Philippe, Germain, Adeline, Helder, Remi, Augot, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672183/
https://www.ncbi.nlm.nih.gov/pubmed/26664944
http://dx.doi.org/10.3389/fvets.2015.00015
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author Hadj-Henni, Leila
De Meulemeester, Thibaut
Depaquit, Jérôme
Noël, Philippe
Germain, Adeline
Helder, Remi
Augot, Denis
author_facet Hadj-Henni, Leila
De Meulemeester, Thibaut
Depaquit, Jérôme
Noël, Philippe
Germain, Adeline
Helder, Remi
Augot, Denis
author_sort Hadj-Henni, Leila
collection PubMed
description To date, studies on host preferences and blood meal identification have been conducted for Culicoides species using molecular-based methods such as PCR techniques to amplify only a fragment from universal vertebrate mitochondrial genes such as cytochrome c oxidase subunit I or cytochrome b (Cyt b). The vertebrate prepronociceptin gene (PNOC) was also tested in this field. However, the choice of molecular marker to identify blood meal is critical. The objective of our study is to compare the ability of Cyt b and PNOC as molecular markers for blood meal identification depending on the stage of blood meal digestion. In order to determine whether these Cyt b and PNOC could provide a positive result, 565 blood-fed females of Culicoides spp were collected and morphologically identified. The samples were collected between 2012 and 2014, in two localities in France. The collection localities were near either livestock or a forest. To catch the specimens, we used UV CDC miniature light traps. PNOC sequence of donkeys (Equus asinus) was sequenced and submitted because it was missing in GenBank. Our findings emphasize that the PNOC marker is not suitable to separate closely related Equid species such as horses and donkeys. The Cyt b marker was able to identify 204 more samples when compared to PNOC (99.55% of specimens). Cyt b appears to be better able to detect the origin of blood meals from females with digested blood in their abdomens. We conclude that Cyt b is a good marker as it increases the accuracy of blood meal identification of engorged females containing digested blood in their abdomens. The host opportunist behavior of Culicoides, especially that of C. obsoletus and C. scoticus, the main vectors of BTV in Europe was also highlighted.
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spelling pubmed-46721832015-12-10 Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides Hadj-Henni, Leila De Meulemeester, Thibaut Depaquit, Jérôme Noël, Philippe Germain, Adeline Helder, Remi Augot, Denis Front Vet Sci Veterinary Science To date, studies on host preferences and blood meal identification have been conducted for Culicoides species using molecular-based methods such as PCR techniques to amplify only a fragment from universal vertebrate mitochondrial genes such as cytochrome c oxidase subunit I or cytochrome b (Cyt b). The vertebrate prepronociceptin gene (PNOC) was also tested in this field. However, the choice of molecular marker to identify blood meal is critical. The objective of our study is to compare the ability of Cyt b and PNOC as molecular markers for blood meal identification depending on the stage of blood meal digestion. In order to determine whether these Cyt b and PNOC could provide a positive result, 565 blood-fed females of Culicoides spp were collected and morphologically identified. The samples were collected between 2012 and 2014, in two localities in France. The collection localities were near either livestock or a forest. To catch the specimens, we used UV CDC miniature light traps. PNOC sequence of donkeys (Equus asinus) was sequenced and submitted because it was missing in GenBank. Our findings emphasize that the PNOC marker is not suitable to separate closely related Equid species such as horses and donkeys. The Cyt b marker was able to identify 204 more samples when compared to PNOC (99.55% of specimens). Cyt b appears to be better able to detect the origin of blood meals from females with digested blood in their abdomens. We conclude that Cyt b is a good marker as it increases the accuracy of blood meal identification of engorged females containing digested blood in their abdomens. The host opportunist behavior of Culicoides, especially that of C. obsoletus and C. scoticus, the main vectors of BTV in Europe was also highlighted. Frontiers Media S.A. 2015-05-27 /pmc/articles/PMC4672183/ /pubmed/26664944 http://dx.doi.org/10.3389/fvets.2015.00015 Text en Copyright © 2015 Hadj-Henni, De Meulemeester, Depaquit, Noël, Germain, Helder and Augot. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Hadj-Henni, Leila
De Meulemeester, Thibaut
Depaquit, Jérôme
Noël, Philippe
Germain, Adeline
Helder, Remi
Augot, Denis
Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides
title Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides
title_full Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides
title_fullStr Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides
title_full_unstemmed Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides
title_short Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides
title_sort comparison of vertebrate cytochrome b and prepronociceptin for blood meal analyses in culicoides
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672183/
https://www.ncbi.nlm.nih.gov/pubmed/26664944
http://dx.doi.org/10.3389/fvets.2015.00015
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