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Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice

BACKGROUND: The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential...

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Autores principales: Rodriguez, Jonathan, Boucher, Fabien, Lequeux, Charlotte, Josset-Lamaugarny, Audrey, Rouyer, Ondine, Ardisson, Orianne, Rutschi, Héléna, Sigaudo-Roussel, Dominique, Damour, Odile, Mojallal, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672563/
https://www.ncbi.nlm.nih.gov/pubmed/26645735
http://dx.doi.org/10.1186/s13287-015-0238-3
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author Rodriguez, Jonathan
Boucher, Fabien
Lequeux, Charlotte
Josset-Lamaugarny, Audrey
Rouyer, Ondine
Ardisson, Orianne
Rutschi, Héléna
Sigaudo-Roussel, Dominique
Damour, Odile
Mojallal, Ali
author_facet Rodriguez, Jonathan
Boucher, Fabien
Lequeux, Charlotte
Josset-Lamaugarny, Audrey
Rouyer, Ondine
Ardisson, Orianne
Rutschi, Héléna
Sigaudo-Roussel, Dominique
Damour, Odile
Mojallal, Ali
author_sort Rodriguez, Jonathan
collection PubMed
description BACKGROUND: The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential effect of ASCs on the healing of 12 mm diameter-excisional wounds (around 110 mm(2)) in nude mice. METHODS: Thirty nude mice underwent surgery to create one 12-mm excisional wound per mouse (spontaneous healing, n = 6; Cytocare® 532, n = 12; ASCs, n = 12). The Galiano wound model was chosen to avoid shrinkage and thus slow the spontaneous healing (SH) of mouse skin, making it closer to the physiology of human skin healing. Transparent dressings were used to enable daily healing time measurements to be taken. Immunohistochemistry, histological and blood perfusion analysis were carried out on the healed skin. RESULTS: The in vivo results showed the effectiveness of using ASCs on reducing the time needed for complete healing to 21.2 days for SH, 17.4 days for vehicle alone (Cytocare® 532) and 14.6 days with the addition of ASCs (p < 0.001). Moreover, cutaneous perfusion of the healed wound was significantly improved in ASC-treated mice compared to SH group, as shown by laser Doppler flowmetry and the quantitation of blood vessels using immunohistochemistry of αsmooth muscle actin. CONCLUSIONS: The tolerance and efficacy of cryopreserved ASCs to accelerate the complete closure of the wound by increasing the maturation of the skin and its blood perfusion, shows their therapeutic benefit in the wound healing context.
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spelling pubmed-46725632015-12-09 Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice Rodriguez, Jonathan Boucher, Fabien Lequeux, Charlotte Josset-Lamaugarny, Audrey Rouyer, Ondine Ardisson, Orianne Rutschi, Héléna Sigaudo-Roussel, Dominique Damour, Odile Mojallal, Ali Stem Cell Res Ther Research BACKGROUND: The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential effect of ASCs on the healing of 12 mm diameter-excisional wounds (around 110 mm(2)) in nude mice. METHODS: Thirty nude mice underwent surgery to create one 12-mm excisional wound per mouse (spontaneous healing, n = 6; Cytocare® 532, n = 12; ASCs, n = 12). The Galiano wound model was chosen to avoid shrinkage and thus slow the spontaneous healing (SH) of mouse skin, making it closer to the physiology of human skin healing. Transparent dressings were used to enable daily healing time measurements to be taken. Immunohistochemistry, histological and blood perfusion analysis were carried out on the healed skin. RESULTS: The in vivo results showed the effectiveness of using ASCs on reducing the time needed for complete healing to 21.2 days for SH, 17.4 days for vehicle alone (Cytocare® 532) and 14.6 days with the addition of ASCs (p < 0.001). Moreover, cutaneous perfusion of the healed wound was significantly improved in ASC-treated mice compared to SH group, as shown by laser Doppler flowmetry and the quantitation of blood vessels using immunohistochemistry of αsmooth muscle actin. CONCLUSIONS: The tolerance and efficacy of cryopreserved ASCs to accelerate the complete closure of the wound by increasing the maturation of the skin and its blood perfusion, shows their therapeutic benefit in the wound healing context. BioMed Central 2015-12-08 /pmc/articles/PMC4672563/ /pubmed/26645735 http://dx.doi.org/10.1186/s13287-015-0238-3 Text en © Rodriguez et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Rodriguez, Jonathan
Boucher, Fabien
Lequeux, Charlotte
Josset-Lamaugarny, Audrey
Rouyer, Ondine
Ardisson, Orianne
Rutschi, Héléna
Sigaudo-Roussel, Dominique
Damour, Odile
Mojallal, Ali
Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
title Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
title_full Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
title_fullStr Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
title_full_unstemmed Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
title_short Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
title_sort intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672563/
https://www.ncbi.nlm.nih.gov/pubmed/26645735
http://dx.doi.org/10.1186/s13287-015-0238-3
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