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Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
BACKGROUND: The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672563/ https://www.ncbi.nlm.nih.gov/pubmed/26645735 http://dx.doi.org/10.1186/s13287-015-0238-3 |
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author | Rodriguez, Jonathan Boucher, Fabien Lequeux, Charlotte Josset-Lamaugarny, Audrey Rouyer, Ondine Ardisson, Orianne Rutschi, Héléna Sigaudo-Roussel, Dominique Damour, Odile Mojallal, Ali |
author_facet | Rodriguez, Jonathan Boucher, Fabien Lequeux, Charlotte Josset-Lamaugarny, Audrey Rouyer, Ondine Ardisson, Orianne Rutschi, Héléna Sigaudo-Roussel, Dominique Damour, Odile Mojallal, Ali |
author_sort | Rodriguez, Jonathan |
collection | PubMed |
description | BACKGROUND: The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential effect of ASCs on the healing of 12 mm diameter-excisional wounds (around 110 mm(2)) in nude mice. METHODS: Thirty nude mice underwent surgery to create one 12-mm excisional wound per mouse (spontaneous healing, n = 6; Cytocare® 532, n = 12; ASCs, n = 12). The Galiano wound model was chosen to avoid shrinkage and thus slow the spontaneous healing (SH) of mouse skin, making it closer to the physiology of human skin healing. Transparent dressings were used to enable daily healing time measurements to be taken. Immunohistochemistry, histological and blood perfusion analysis were carried out on the healed skin. RESULTS: The in vivo results showed the effectiveness of using ASCs on reducing the time needed for complete healing to 21.2 days for SH, 17.4 days for vehicle alone (Cytocare® 532) and 14.6 days with the addition of ASCs (p < 0.001). Moreover, cutaneous perfusion of the healed wound was significantly improved in ASC-treated mice compared to SH group, as shown by laser Doppler flowmetry and the quantitation of blood vessels using immunohistochemistry of αsmooth muscle actin. CONCLUSIONS: The tolerance and efficacy of cryopreserved ASCs to accelerate the complete closure of the wound by increasing the maturation of the skin and its blood perfusion, shows their therapeutic benefit in the wound healing context. |
format | Online Article Text |
id | pubmed-4672563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46725632015-12-09 Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice Rodriguez, Jonathan Boucher, Fabien Lequeux, Charlotte Josset-Lamaugarny, Audrey Rouyer, Ondine Ardisson, Orianne Rutschi, Héléna Sigaudo-Roussel, Dominique Damour, Odile Mojallal, Ali Stem Cell Res Ther Research BACKGROUND: The use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential effect of ASCs on the healing of 12 mm diameter-excisional wounds (around 110 mm(2)) in nude mice. METHODS: Thirty nude mice underwent surgery to create one 12-mm excisional wound per mouse (spontaneous healing, n = 6; Cytocare® 532, n = 12; ASCs, n = 12). The Galiano wound model was chosen to avoid shrinkage and thus slow the spontaneous healing (SH) of mouse skin, making it closer to the physiology of human skin healing. Transparent dressings were used to enable daily healing time measurements to be taken. Immunohistochemistry, histological and blood perfusion analysis were carried out on the healed skin. RESULTS: The in vivo results showed the effectiveness of using ASCs on reducing the time needed for complete healing to 21.2 days for SH, 17.4 days for vehicle alone (Cytocare® 532) and 14.6 days with the addition of ASCs (p < 0.001). Moreover, cutaneous perfusion of the healed wound was significantly improved in ASC-treated mice compared to SH group, as shown by laser Doppler flowmetry and the quantitation of blood vessels using immunohistochemistry of αsmooth muscle actin. CONCLUSIONS: The tolerance and efficacy of cryopreserved ASCs to accelerate the complete closure of the wound by increasing the maturation of the skin and its blood perfusion, shows their therapeutic benefit in the wound healing context. BioMed Central 2015-12-08 /pmc/articles/PMC4672563/ /pubmed/26645735 http://dx.doi.org/10.1186/s13287-015-0238-3 Text en © Rodriguez et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Rodriguez, Jonathan Boucher, Fabien Lequeux, Charlotte Josset-Lamaugarny, Audrey Rouyer, Ondine Ardisson, Orianne Rutschi, Héléna Sigaudo-Roussel, Dominique Damour, Odile Mojallal, Ali Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice |
title | Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice |
title_full | Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice |
title_fullStr | Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice |
title_full_unstemmed | Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice |
title_short | Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice |
title_sort | intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672563/ https://www.ncbi.nlm.nih.gov/pubmed/26645735 http://dx.doi.org/10.1186/s13287-015-0238-3 |
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