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The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields
Purpose: Following in utero exposure to low dose radiation (10–200 mGy), we recently observed a linear induction of DNA double-strand breaks (DSB) and activation of apoptosis in the embryonic neuronal stem/progenitor cell compartment. No significant induction of DSB or apoptosis was observed followi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673581/ https://www.ncbi.nlm.nih.gov/pubmed/25786477 http://dx.doi.org/10.3109/09553002.2015.1021963 |
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author | Woodbine, Lisa Haines, Jackie Coster, Margaret Barazzuol, Lara Ainsbury, Elizabeth Sienkiewicz, Zenon Jeggo, Penny |
author_facet | Woodbine, Lisa Haines, Jackie Coster, Margaret Barazzuol, Lara Ainsbury, Elizabeth Sienkiewicz, Zenon Jeggo, Penny |
author_sort | Woodbine, Lisa |
collection | PubMed |
description | Purpose: Following in utero exposure to low dose radiation (10–200 mGy), we recently observed a linear induction of DNA double-strand breaks (DSB) and activation of apoptosis in the embryonic neuronal stem/progenitor cell compartment. No significant induction of DSB or apoptosis was observed following exposure to magnetic fields (MF). In the present study, we exploited this in vivo system to examine whether exposure to MF before and after exposure to 100 mGy X-rays impacts upon DSB repair rates. Materials and methods: 53BP1 foci were quantified following combined exposure to radiation and MF in the embryonic neuronal stem/progenitor cell compartment. Embryos were exposed in utero to 50 Hz MF at 300 μT for 3 h before and up to 9 h after exposure to 100 mGy X-rays. Controls included embryos exposed to MF or X-rays alone plus sham exposures. Results: Exposure to MF before and after 100 mGy X-rays did not impact upon the rate of DSB repair in the embryonic neuronal stem cell compartment compared to repair rates following radiation exposure alone. Conclusions: We conclude that in this sensitive system MF do not exert any significant level of DNA damage and do not impede the repair of X-ray induced damage. |
format | Online Article Text |
id | pubmed-4673581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-46735812015-12-15 The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields Woodbine, Lisa Haines, Jackie Coster, Margaret Barazzuol, Lara Ainsbury, Elizabeth Sienkiewicz, Zenon Jeggo, Penny Int J Radiat Biol DNA Double Strand Break Repair and Magnetic Field Exposure Purpose: Following in utero exposure to low dose radiation (10–200 mGy), we recently observed a linear induction of DNA double-strand breaks (DSB) and activation of apoptosis in the embryonic neuronal stem/progenitor cell compartment. No significant induction of DSB or apoptosis was observed following exposure to magnetic fields (MF). In the present study, we exploited this in vivo system to examine whether exposure to MF before and after exposure to 100 mGy X-rays impacts upon DSB repair rates. Materials and methods: 53BP1 foci were quantified following combined exposure to radiation and MF in the embryonic neuronal stem/progenitor cell compartment. Embryos were exposed in utero to 50 Hz MF at 300 μT for 3 h before and up to 9 h after exposure to 100 mGy X-rays. Controls included embryos exposed to MF or X-rays alone plus sham exposures. Results: Exposure to MF before and after 100 mGy X-rays did not impact upon the rate of DSB repair in the embryonic neuronal stem cell compartment compared to repair rates following radiation exposure alone. Conclusions: We conclude that in this sensitive system MF do not exert any significant level of DNA damage and do not impede the repair of X-ray induced damage. Taylor & Francis 2015-06-03 2015-03-28 /pmc/articles/PMC4673581/ /pubmed/25786477 http://dx.doi.org/10.3109/09553002.2015.1021963 Text en © 2015 The Author(s). Published by Taylor & Francis. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | DNA Double Strand Break Repair and Magnetic Field Exposure Woodbine, Lisa Haines, Jackie Coster, Margaret Barazzuol, Lara Ainsbury, Elizabeth Sienkiewicz, Zenon Jeggo, Penny The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields |
title | The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields |
title_full | The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields |
title_fullStr | The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields |
title_full_unstemmed | The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields |
title_short | The rate of X-ray-induced DNA double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 Hz magnetic fields |
title_sort | rate of x-ray-induced dna double-strand break repair in the embryonic mouse brain is unaffected by exposure to 50 hz magnetic fields |
topic | DNA Double Strand Break Repair and Magnetic Field Exposure |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673581/ https://www.ncbi.nlm.nih.gov/pubmed/25786477 http://dx.doi.org/10.3109/09553002.2015.1021963 |
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