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HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV

BACKGROUND: Whilst ART corrects many effects of HIV disease, T cell populations retain features of accelerated immunological aging. METHODS: Here we analyse phenotypic changes to natural killer (NK) cells in HIV patients who began ART with <200 CD4 T-cells/µl and maintained virological control fo...

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Autores principales: Affandi, Jacquita S., Montgomery, Jacinta, Lee, Silvia, Price, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673723/
https://www.ncbi.nlm.nih.gov/pubmed/26664457
http://dx.doi.org/10.1186/s12981-015-0080-9
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author Affandi, Jacquita S.
Montgomery, Jacinta
Lee, Silvia
Price, Patricia
author_facet Affandi, Jacquita S.
Montgomery, Jacinta
Lee, Silvia
Price, Patricia
author_sort Affandi, Jacquita S.
collection PubMed
description BACKGROUND: Whilst ART corrects many effects of HIV disease, T cell populations retain features of accelerated immunological aging. METHODS: Here we analyse phenotypic changes to natural killer (NK) cells in HIV patients who began ART with <200 CD4 T-cells/µl and maintained virological control for 12–17 years, compared with CMV seropositive and seronegative healthy control donors. RESULTS: Humoral responses to CMV antigens (lysate, gB, IE-1) remain elevated in the patients (P < 0.0001) despite the long duration of ART. Patient’s NK cells responded poorly to K562 cells when assessed by CD107a and IFNγ, but this could not be attributed to CMV as responses were low in CMV-seronegative controls. Moreover HIV (and not CMV) increased expression of CD57 on CD56(lo) cells. CONCLUSIONS: Comparisons with published studies suggest that CMV accelerates age-related increases in CD57 expression but levels plateau by 60–70 years of age, so the effect of CMV disappears. In HIV patients the plateau is higher and perhaps reached sooner. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12981-015-0080-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-46737232015-12-10 HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV Affandi, Jacquita S. Montgomery, Jacinta Lee, Silvia Price, Patricia AIDS Res Ther Research BACKGROUND: Whilst ART corrects many effects of HIV disease, T cell populations retain features of accelerated immunological aging. METHODS: Here we analyse phenotypic changes to natural killer (NK) cells in HIV patients who began ART with <200 CD4 T-cells/µl and maintained virological control for 12–17 years, compared with CMV seropositive and seronegative healthy control donors. RESULTS: Humoral responses to CMV antigens (lysate, gB, IE-1) remain elevated in the patients (P < 0.0001) despite the long duration of ART. Patient’s NK cells responded poorly to K562 cells when assessed by CD107a and IFNγ, but this could not be attributed to CMV as responses were low in CMV-seronegative controls. Moreover HIV (and not CMV) increased expression of CD57 on CD56(lo) cells. CONCLUSIONS: Comparisons with published studies suggest that CMV accelerates age-related increases in CD57 expression but levels plateau by 60–70 years of age, so the effect of CMV disappears. In HIV patients the plateau is higher and perhaps reached sooner. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12981-015-0080-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-12-09 /pmc/articles/PMC4673723/ /pubmed/26664457 http://dx.doi.org/10.1186/s12981-015-0080-9 Text en © Affandi et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Affandi, Jacquita S.
Montgomery, Jacinta
Lee, Silvia
Price, Patricia
HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV
title HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV
title_full HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV
title_fullStr HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV
title_full_unstemmed HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV
title_short HIV patients stable on ART retain evidence of a high CMV load but changes to Natural Killer cell phenotypes reflect both HIV and CMV
title_sort hiv patients stable on art retain evidence of a high cmv load but changes to natural killer cell phenotypes reflect both hiv and cmv
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673723/
https://www.ncbi.nlm.nih.gov/pubmed/26664457
http://dx.doi.org/10.1186/s12981-015-0080-9
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