Cargando…

Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

BACKGROUND: Next-generation transcriptome sequencing (RNA-Seq) has become the standard practice for studying gene splicing, mutations and changes in gene expression to obtain valuable, accurate biological conclusions. However, obtaining good sequencing coverage and depth to study these is impeded by...

Descripción completa

Detalles Bibliográficos
Autores principales: Heera, Rajandas, Sivachandran, Parimannan, Chinni, Suresh V., Mason, Joanne, Croft, Larry, Ravichandran, Manickam, Su Yin, Lee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673735/
https://www.ncbi.nlm.nih.gov/pubmed/26645211
http://dx.doi.org/10.1186/s13104-015-1726-3