Cargando…

High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing

BACKGROUND: High-density map is a valuable tool for genetic and genomic analysis. Although wax gourd is a widely distributed vegetable of Cucurbitaceae and has important medicinal and health value, no genetic map has been constructed because of the lack of efficient markers. Specific-locus amplified...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Biao, Liu, Wenrui, Xie, Dasen, Peng, Qingwu, He, Xiaoming, Lin, Yu’e, Liang, Zhaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673774/
https://www.ncbi.nlm.nih.gov/pubmed/26647294
http://dx.doi.org/10.1186/s12864-015-2220-y
_version_ 1782404805506891776
author Jiang, Biao
Liu, Wenrui
Xie, Dasen
Peng, Qingwu
He, Xiaoming
Lin, Yu’e
Liang, Zhaojun
author_facet Jiang, Biao
Liu, Wenrui
Xie, Dasen
Peng, Qingwu
He, Xiaoming
Lin, Yu’e
Liang, Zhaojun
author_sort Jiang, Biao
collection PubMed
description BACKGROUND: High-density map is a valuable tool for genetic and genomic analysis. Although wax gourd is a widely distributed vegetable of Cucurbitaceae and has important medicinal and health value, no genetic map has been constructed because of the lack of efficient markers. Specific-locus amplified fragment sequencing (SLAF-seq) is a newly developed high-throughput strategy for large-scale single nucleotide polymorphism (SNP) discovery and genotyping. RESULTS: In our present study, we constructed a high-density genetic map by using SLAF-seq and identified a locus controlling pericarp color in wax gourd. An F2 population of 140 individuals and their two parents were subjected to SLAF-seq. A total of 143.38 M pair-end reads were generated. The average sequencing depth was 26.51 in the maternal line (B214), 27.01 in the parental line (B227), and 5.11 in each F2 individual. When filtering low-depth SLAF tags, a total of 142,653 high-quality SLAFs were detected, and 22,151 of them were polymorphic, with a polymorphism rate of 15.42 %. And finally, 4,607 of the polymorphic markers were selected for genetic map construction, and 12 linkage groups (LGs) were generated. The map spanned 2,172.86 cM with an average distance between adjacent markers for 0.49 cM. The inheritance of pericarp color was also studied, which showed that the pericarp color was controlled by one single gene. And based on the newly constructed high-density map, a single locus locating on chromosome 5 was identified for controlling the pericarp color of wax gourd. CONCLUSIONS: This is the first report of high-density genetic map construction and gene mapping in wax gourd, which will be served as an invaluable tool for gene mapping, marker assisted breeding, map-based gene cloning, comparative mapping and draft genome assembling of wax gourd. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-2220-y) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4673774
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-46737742015-12-10 High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing Jiang, Biao Liu, Wenrui Xie, Dasen Peng, Qingwu He, Xiaoming Lin, Yu’e Liang, Zhaojun BMC Genomics Research Article BACKGROUND: High-density map is a valuable tool for genetic and genomic analysis. Although wax gourd is a widely distributed vegetable of Cucurbitaceae and has important medicinal and health value, no genetic map has been constructed because of the lack of efficient markers. Specific-locus amplified fragment sequencing (SLAF-seq) is a newly developed high-throughput strategy for large-scale single nucleotide polymorphism (SNP) discovery and genotyping. RESULTS: In our present study, we constructed a high-density genetic map by using SLAF-seq and identified a locus controlling pericarp color in wax gourd. An F2 population of 140 individuals and their two parents were subjected to SLAF-seq. A total of 143.38 M pair-end reads were generated. The average sequencing depth was 26.51 in the maternal line (B214), 27.01 in the parental line (B227), and 5.11 in each F2 individual. When filtering low-depth SLAF tags, a total of 142,653 high-quality SLAFs were detected, and 22,151 of them were polymorphic, with a polymorphism rate of 15.42 %. And finally, 4,607 of the polymorphic markers were selected for genetic map construction, and 12 linkage groups (LGs) were generated. The map spanned 2,172.86 cM with an average distance between adjacent markers for 0.49 cM. The inheritance of pericarp color was also studied, which showed that the pericarp color was controlled by one single gene. And based on the newly constructed high-density map, a single locus locating on chromosome 5 was identified for controlling the pericarp color of wax gourd. CONCLUSIONS: This is the first report of high-density genetic map construction and gene mapping in wax gourd, which will be served as an invaluable tool for gene mapping, marker assisted breeding, map-based gene cloning, comparative mapping and draft genome assembling of wax gourd. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-2220-y) contains supplementary material, which is available to authorized users. BioMed Central 2015-12-09 /pmc/articles/PMC4673774/ /pubmed/26647294 http://dx.doi.org/10.1186/s12864-015-2220-y Text en © Jiang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jiang, Biao
Liu, Wenrui
Xie, Dasen
Peng, Qingwu
He, Xiaoming
Lin, Yu’e
Liang, Zhaojun
High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing
title High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing
title_full High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing
title_fullStr High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing
title_full_unstemmed High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing
title_short High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing
title_sort high-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (slaf) sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673774/
https://www.ncbi.nlm.nih.gov/pubmed/26647294
http://dx.doi.org/10.1186/s12864-015-2220-y
work_keys_str_mv AT jiangbiao highdensitygeneticmapconstructionandgenemappingofpericarpcolorinwaxgourdusingspecificlocusamplifiedfragmentslafsequencing
AT liuwenrui highdensitygeneticmapconstructionandgenemappingofpericarpcolorinwaxgourdusingspecificlocusamplifiedfragmentslafsequencing
AT xiedasen highdensitygeneticmapconstructionandgenemappingofpericarpcolorinwaxgourdusingspecificlocusamplifiedfragmentslafsequencing
AT pengqingwu highdensitygeneticmapconstructionandgenemappingofpericarpcolorinwaxgourdusingspecificlocusamplifiedfragmentslafsequencing
AT hexiaoming highdensitygeneticmapconstructionandgenemappingofpericarpcolorinwaxgourdusingspecificlocusamplifiedfragmentslafsequencing
AT linyue highdensitygeneticmapconstructionandgenemappingofpericarpcolorinwaxgourdusingspecificlocusamplifiedfragmentslafsequencing
AT liangzhaojun highdensitygeneticmapconstructionandgenemappingofpericarpcolorinwaxgourdusingspecificlocusamplifiedfragmentslafsequencing