Cargando…

Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression

Developments of non-viral carriers have headed toward reducing cytotoxicity, which results from the use of conventional gene carriers, and enhancing gene delivery efficiency. Cys-(d-R9)-Cys repeated reducible poly(oligo-D-arginine) (rPOA) is one of the most efficient non-viral carriers for gene ther...

Descripción completa

Detalles Bibliográficos
Autores principales: Woo, Jongsu, Bae, Seong-Ho, Kim, Bokyoung, Park, Jin Sil, Jung, Subin, Lee, Minhyung, Kim, Yong-Hee, Choi, Donghoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674056/
https://www.ncbi.nlm.nih.gov/pubmed/26649571
http://dx.doi.org/10.1371/journal.pone.0144491
_version_ 1782404850649137152
author Woo, Jongsu
Bae, Seong-Ho
Kim, Bokyoung
Park, Jin Sil
Jung, Subin
Lee, Minhyung
Kim, Yong-Hee
Choi, Donghoon
author_facet Woo, Jongsu
Bae, Seong-Ho
Kim, Bokyoung
Park, Jin Sil
Jung, Subin
Lee, Minhyung
Kim, Yong-Hee
Choi, Donghoon
author_sort Woo, Jongsu
collection PubMed
description Developments of non-viral carriers have headed toward reducing cytotoxicity, which results from the use of conventional gene carriers, and enhancing gene delivery efficiency. Cys-(d-R9)-Cys repeated reducible poly(oligo-D-arginine) (rPOA) is one of the most efficient non-viral carriers for gene therapy; however, while its efficiency has been verified in the lung and brain, it is necessary to confirm its activity in each organ or tissue since there are differences of gene carrier susceptibility to among tissue types. We therefore tested the compatibility of rPOA in cardiac tissue by in vitro or in vivo experiments and confirmed its high transfection efficiency and low cytotoxicity. Moreover, substantial regenerative effects were observed following transfection with rPOA/pVEGF expression vector complexes (79% decreased infarct size) compared to polyethyleneimine (PEI) (34% decreased infarct size) in a rat myocardial infarction (MI) model. These findings suggest that rPOA efficiently enables DNA transfection in cardiac tissue and can be used as a useful non-viral therapeutic gene carrier for gene therapy in ischemic heart disease.
format Online
Article
Text
id pubmed-4674056
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-46740562015-12-23 Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression Woo, Jongsu Bae, Seong-Ho Kim, Bokyoung Park, Jin Sil Jung, Subin Lee, Minhyung Kim, Yong-Hee Choi, Donghoon PLoS One Research Article Developments of non-viral carriers have headed toward reducing cytotoxicity, which results from the use of conventional gene carriers, and enhancing gene delivery efficiency. Cys-(d-R9)-Cys repeated reducible poly(oligo-D-arginine) (rPOA) is one of the most efficient non-viral carriers for gene therapy; however, while its efficiency has been verified in the lung and brain, it is necessary to confirm its activity in each organ or tissue since there are differences of gene carrier susceptibility to among tissue types. We therefore tested the compatibility of rPOA in cardiac tissue by in vitro or in vivo experiments and confirmed its high transfection efficiency and low cytotoxicity. Moreover, substantial regenerative effects were observed following transfection with rPOA/pVEGF expression vector complexes (79% decreased infarct size) compared to polyethyleneimine (PEI) (34% decreased infarct size) in a rat myocardial infarction (MI) model. These findings suggest that rPOA efficiently enables DNA transfection in cardiac tissue and can be used as a useful non-viral therapeutic gene carrier for gene therapy in ischemic heart disease. Public Library of Science 2015-12-09 /pmc/articles/PMC4674056/ /pubmed/26649571 http://dx.doi.org/10.1371/journal.pone.0144491 Text en © 2015 Woo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Woo, Jongsu
Bae, Seong-Ho
Kim, Bokyoung
Park, Jin Sil
Jung, Subin
Lee, Minhyung
Kim, Yong-Hee
Choi, Donghoon
Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression
title Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression
title_full Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression
title_fullStr Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression
title_full_unstemmed Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression
title_short Cardiac Usage of Reducible Poly(oligo-D-arginine) As a Gene Carrier for Vascular Endothelial Growth Factor Expression
title_sort cardiac usage of reducible poly(oligo-d-arginine) as a gene carrier for vascular endothelial growth factor expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674056/
https://www.ncbi.nlm.nih.gov/pubmed/26649571
http://dx.doi.org/10.1371/journal.pone.0144491
work_keys_str_mv AT woojongsu cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression
AT baeseongho cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression
AT kimbokyoung cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression
AT parkjinsil cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression
AT jungsubin cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression
AT leeminhyung cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression
AT kimyonghee cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression
AT choidonghoon cardiacusageofreduciblepolyoligodarginineasagenecarrierforvascularendothelialgrowthfactorexpression