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AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport

AtNHX5 and AtNHX6, the endosomal Na(+),K(+)/H(+) antiporters in Arabidopsis, play an important role in plant growth and development. However, their function in K(+) and pH homeostasis remains unclear. In this report, we characterized the function of AtNHX5 and AtNHX6 in K(+) and H(+) homeostasis in...

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Autores principales: Wang, Liguang, Wu, Xuexia, Liu, Yafen, Qiu, Quan-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674129/
https://www.ncbi.nlm.nih.gov/pubmed/26650539
http://dx.doi.org/10.1371/journal.pone.0144716
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author Wang, Liguang
Wu, Xuexia
Liu, Yafen
Qiu, Quan-Sheng
author_facet Wang, Liguang
Wu, Xuexia
Liu, Yafen
Qiu, Quan-Sheng
author_sort Wang, Liguang
collection PubMed
description AtNHX5 and AtNHX6, the endosomal Na(+),K(+)/H(+) antiporters in Arabidopsis, play an important role in plant growth and development. However, their function in K(+) and pH homeostasis remains unclear. In this report, we characterized the function of AtNHX5 and AtNHX6 in K(+) and H(+) homeostasis in Arabidopsis. Using a yeast expression system, we found that AtNHX5 and AtNHX6 recovered tolerance to high K(+) or salt. We further found that AtNHX5 and AtNHX6 functioned at high K(+) at acidic pH while AtCHXs at low K(+) under alkaline conditions. In addition, we showed that the nhx5 nhx6 double mutant contained less K(+) and was sensitive to low K(+) treatment. Overexpression of AtNHX5 or AtNHX6 gene in nhx5 nhx6 recovered root growth to the wild-type level. Three conserved acidic residues, D164, E188, and D193 in AtNHX5 and D165, E189, and D194 in AtNHX6, were essential for K(+) homeostasis and plant growth. nhx5 nhx6 had a reduced vacuolar and cellular pH as measured with the fluorescent pH indicator BCECF or semimicroelectrode. We further show that AtNHX5 and AtNHX6 are localized to Golgi and TGN. Taken together, AtNHX5 and AtNHX6 play an important role in K(+) and pH homeostasis in Arabidopsis. Three conserved acidic residues are essential for K(+) transport.
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spelling pubmed-46741292015-12-23 AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport Wang, Liguang Wu, Xuexia Liu, Yafen Qiu, Quan-Sheng PLoS One Research Article AtNHX5 and AtNHX6, the endosomal Na(+),K(+)/H(+) antiporters in Arabidopsis, play an important role in plant growth and development. However, their function in K(+) and pH homeostasis remains unclear. In this report, we characterized the function of AtNHX5 and AtNHX6 in K(+) and H(+) homeostasis in Arabidopsis. Using a yeast expression system, we found that AtNHX5 and AtNHX6 recovered tolerance to high K(+) or salt. We further found that AtNHX5 and AtNHX6 functioned at high K(+) at acidic pH while AtCHXs at low K(+) under alkaline conditions. In addition, we showed that the nhx5 nhx6 double mutant contained less K(+) and was sensitive to low K(+) treatment. Overexpression of AtNHX5 or AtNHX6 gene in nhx5 nhx6 recovered root growth to the wild-type level. Three conserved acidic residues, D164, E188, and D193 in AtNHX5 and D165, E189, and D194 in AtNHX6, were essential for K(+) homeostasis and plant growth. nhx5 nhx6 had a reduced vacuolar and cellular pH as measured with the fluorescent pH indicator BCECF or semimicroelectrode. We further show that AtNHX5 and AtNHX6 are localized to Golgi and TGN. Taken together, AtNHX5 and AtNHX6 play an important role in K(+) and pH homeostasis in Arabidopsis. Three conserved acidic residues are essential for K(+) transport. Public Library of Science 2015-12-09 /pmc/articles/PMC4674129/ /pubmed/26650539 http://dx.doi.org/10.1371/journal.pone.0144716 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Liguang
Wu, Xuexia
Liu, Yafen
Qiu, Quan-Sheng
AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport
title AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport
title_full AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport
title_fullStr AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport
title_full_unstemmed AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport
title_short AtNHX5 and AtNHX6 Control Cellular K(+) and pH Homeostasis in Arabidopsis: Three Conserved Acidic Residues Are Essential for K(+) Transport
title_sort atnhx5 and atnhx6 control cellular k(+) and ph homeostasis in arabidopsis: three conserved acidic residues are essential for k(+) transport
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674129/
https://www.ncbi.nlm.nih.gov/pubmed/26650539
http://dx.doi.org/10.1371/journal.pone.0144716
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