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Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes

Intracellular transport is mediated by molecular motors that bind cargo to be transported along the cytoskeleton. Here, we report, for the first time, that peroxisomes (POs), lipid droplets (LDs), and the endoplasmic reticulum (ER) rely on early endosomes (EEs) for intracellular movement in a fungal...

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Autores principales: Guimaraes, Sofia C., Schuster, Martin, Bielska, Ewa, Dagdas, Gulay, Kilaru, Sreedhar, Meadows, Ben R.A., Schrader, Michael, Steinberg, Gero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674278/
https://www.ncbi.nlm.nih.gov/pubmed/26620910
http://dx.doi.org/10.1083/jcb.201505086
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author Guimaraes, Sofia C.
Schuster, Martin
Bielska, Ewa
Dagdas, Gulay
Kilaru, Sreedhar
Meadows, Ben R.A.
Schrader, Michael
Steinberg, Gero
author_facet Guimaraes, Sofia C.
Schuster, Martin
Bielska, Ewa
Dagdas, Gulay
Kilaru, Sreedhar
Meadows, Ben R.A.
Schrader, Michael
Steinberg, Gero
author_sort Guimaraes, Sofia C.
collection PubMed
description Intracellular transport is mediated by molecular motors that bind cargo to be transported along the cytoskeleton. Here, we report, for the first time, that peroxisomes (POs), lipid droplets (LDs), and the endoplasmic reticulum (ER) rely on early endosomes (EEs) for intracellular movement in a fungal model system. We show that POs undergo kinesin-3– and dynein-dependent transport along microtubules. Surprisingly, kinesin-3 does not colocalize with POs. Instead, the motor moves EEs that drag the POs through the cell. PO motility is abolished when EE motility is blocked in various mutants. Most LD and ER motility also depends on EE motility, whereas mitochondria move independently of EEs. Covisualization studies show that EE-mediated ER motility is not required for PO or LD movement, suggesting that the organelles interact with EEs independently. In the absence of EE motility, POs and LDs cluster at the growing tip, whereas ER is partially retracted to subapical regions. Collectively, our results show that moving EEs interact transiently with other organelles, thereby mediating their directed transport and distribution in the cell.
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spelling pubmed-46742782016-06-07 Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes Guimaraes, Sofia C. Schuster, Martin Bielska, Ewa Dagdas, Gulay Kilaru, Sreedhar Meadows, Ben R.A. Schrader, Michael Steinberg, Gero J Cell Biol Research Articles Intracellular transport is mediated by molecular motors that bind cargo to be transported along the cytoskeleton. Here, we report, for the first time, that peroxisomes (POs), lipid droplets (LDs), and the endoplasmic reticulum (ER) rely on early endosomes (EEs) for intracellular movement in a fungal model system. We show that POs undergo kinesin-3– and dynein-dependent transport along microtubules. Surprisingly, kinesin-3 does not colocalize with POs. Instead, the motor moves EEs that drag the POs through the cell. PO motility is abolished when EE motility is blocked in various mutants. Most LD and ER motility also depends on EE motility, whereas mitochondria move independently of EEs. Covisualization studies show that EE-mediated ER motility is not required for PO or LD movement, suggesting that the organelles interact with EEs independently. In the absence of EE motility, POs and LDs cluster at the growing tip, whereas ER is partially retracted to subapical regions. Collectively, our results show that moving EEs interact transiently with other organelles, thereby mediating their directed transport and distribution in the cell. The Rockefeller University Press 2015-12-07 /pmc/articles/PMC4674278/ /pubmed/26620910 http://dx.doi.org/10.1083/jcb.201505086 Text en © 2015 Guimaraes et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Guimaraes, Sofia C.
Schuster, Martin
Bielska, Ewa
Dagdas, Gulay
Kilaru, Sreedhar
Meadows, Ben R.A.
Schrader, Michael
Steinberg, Gero
Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
title Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
title_full Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
title_fullStr Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
title_full_unstemmed Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
title_short Peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
title_sort peroxisomes, lipid droplets, and endoplasmic reticulum “hitchhike” on motile early endosomes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674278/
https://www.ncbi.nlm.nih.gov/pubmed/26620910
http://dx.doi.org/10.1083/jcb.201505086
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