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Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa
Arbuscular mycorrhiza fungi (AMF) can colonize the roots of Amorpha fruticosa, a perennial leguminous woody shrub, and form arbuscular mycorrhiza (AM). AMF have significant promoting effects on A. fruticosa growth as the intensity of fungal colonization increases. Taking AMF-A. fruticosa symbionts a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674871/ https://www.ncbi.nlm.nih.gov/pubmed/26658758 http://dx.doi.org/10.1038/srep18031 |
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author | Song, Fuqiang Qi, Dandan Liu, Xuan Kong, Xiangshi Gao, Yang Zhou, Zixin Wu, Qi |
author_facet | Song, Fuqiang Qi, Dandan Liu, Xuan Kong, Xiangshi Gao, Yang Zhou, Zixin Wu, Qi |
author_sort | Song, Fuqiang |
collection | PubMed |
description | Arbuscular mycorrhiza fungi (AMF) can colonize the roots of Amorpha fruticosa, a perennial leguminous woody shrub, and form arbuscular mycorrhiza (AM). AMF have significant promoting effects on A. fruticosa growth as the intensity of fungal colonization increases. Taking AMF-A. fruticosa symbionts as the experimental material, gel-free isobaric tags for relative and absolute quantification (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to investigate the expression of A. fruticosa mycorrhizal proteins at the maturation stage. A total of 3,473 proteins were identified, of which 77 showed dramatic changes in their root expression levels; 33 increased, and 44 decreased. We also found nine AMF proteins that were expressed with AMF treatment. The 77 proteins were classified according to function. Plant proteins were assigned into 11 categories: metabolism-related (32%), protein folding and degradation-related (22%), energy-related (10%), protein synthesis-related (8%), stress and defense-related (24%), transcription-related (6%), membrane and transport-related (4%), cellular structure-related (2.5%), signaling transduction-related (11%) and unknown proteins (5%). The results of the study provide a foundation for further investigation of the metabolic characteristics and molecular mechanisms of AM. |
format | Online Article Text |
id | pubmed-4674871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46748712015-12-16 Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa Song, Fuqiang Qi, Dandan Liu, Xuan Kong, Xiangshi Gao, Yang Zhou, Zixin Wu, Qi Sci Rep Article Arbuscular mycorrhiza fungi (AMF) can colonize the roots of Amorpha fruticosa, a perennial leguminous woody shrub, and form arbuscular mycorrhiza (AM). AMF have significant promoting effects on A. fruticosa growth as the intensity of fungal colonization increases. Taking AMF-A. fruticosa symbionts as the experimental material, gel-free isobaric tags for relative and absolute quantification (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to investigate the expression of A. fruticosa mycorrhizal proteins at the maturation stage. A total of 3,473 proteins were identified, of which 77 showed dramatic changes in their root expression levels; 33 increased, and 44 decreased. We also found nine AMF proteins that were expressed with AMF treatment. The 77 proteins were classified according to function. Plant proteins were assigned into 11 categories: metabolism-related (32%), protein folding and degradation-related (22%), energy-related (10%), protein synthesis-related (8%), stress and defense-related (24%), transcription-related (6%), membrane and transport-related (4%), cellular structure-related (2.5%), signaling transduction-related (11%) and unknown proteins (5%). The results of the study provide a foundation for further investigation of the metabolic characteristics and molecular mechanisms of AM. Nature Publishing Group 2015-12-10 /pmc/articles/PMC4674871/ /pubmed/26658758 http://dx.doi.org/10.1038/srep18031 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Song, Fuqiang Qi, Dandan Liu, Xuan Kong, Xiangshi Gao, Yang Zhou, Zixin Wu, Qi Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa |
title | Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa |
title_full | Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa |
title_fullStr | Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa |
title_full_unstemmed | Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa |
title_short | Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa |
title_sort | proteomic analysis of symbiotic proteins of glomus mosseae and amorpha fruticosa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674871/ https://www.ncbi.nlm.nih.gov/pubmed/26658758 http://dx.doi.org/10.1038/srep18031 |
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