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High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil

Ensifer medicae WSM244 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago species. WSM244 was isolated in 1979 from a nodule recovered from the roots of the annual Medicago polymorpha L. growing in alkaline soil (p...

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Autores principales: Ardley, Julie, Tian, Rui, O’Hara, Graham, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, Howieson, John, Reeve, Wayne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674904/
https://www.ncbi.nlm.nih.gov/pubmed/26664655
http://dx.doi.org/10.1186/s40793-015-0119-5
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author Ardley, Julie
Tian, Rui
O’Hara, Graham
Seshadri, Rekha
Reddy, T. B. K.
Pati, Amrita
Woyke, Tanja
Markowitz, Victor
Ivanova, Natalia
Kyrpides, Nikos
Howieson, John
Reeve, Wayne
author_facet Ardley, Julie
Tian, Rui
O’Hara, Graham
Seshadri, Rekha
Reddy, T. B. K.
Pati, Amrita
Woyke, Tanja
Markowitz, Victor
Ivanova, Natalia
Kyrpides, Nikos
Howieson, John
Reeve, Wayne
author_sort Ardley, Julie
collection PubMed
description Ensifer medicae WSM244 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago species. WSM244 was isolated in 1979 from a nodule recovered from the roots of the annual Medicago polymorpha L. growing in alkaline soil (pH 8.0) in Tel Afer, Iraq. WSM244 is the only acid-sensitive E. medicae strain that has been sequenced to date. It is effective at fixing nitrogen with M. polymorpha L., as well as with more alkaline-adapted Medicago spp. such as M. littoralis Loisel., M. scutellata (L.) Mill., M. tornata (L.) Mill. and M. truncatula Gaertn. This strain is also effective with the perennial M. sativa L. Here we describe the features of E. medicae WSM244, together with genome sequence information and its annotation. The 6,650,282 bp high-quality permanent draft genome is arranged into 91 scaffolds of 91 contigs containing 6,427 protein-coding genes and 68 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0119-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-46749042015-12-10 High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil Ardley, Julie Tian, Rui O’Hara, Graham Seshadri, Rekha Reddy, T. B. K. Pati, Amrita Woyke, Tanja Markowitz, Victor Ivanova, Natalia Kyrpides, Nikos Howieson, John Reeve, Wayne Stand Genomic Sci Short Genome Report Ensifer medicae WSM244 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago species. WSM244 was isolated in 1979 from a nodule recovered from the roots of the annual Medicago polymorpha L. growing in alkaline soil (pH 8.0) in Tel Afer, Iraq. WSM244 is the only acid-sensitive E. medicae strain that has been sequenced to date. It is effective at fixing nitrogen with M. polymorpha L., as well as with more alkaline-adapted Medicago spp. such as M. littoralis Loisel., M. scutellata (L.) Mill., M. tornata (L.) Mill. and M. truncatula Gaertn. This strain is also effective with the perennial M. sativa L. Here we describe the features of E. medicae WSM244, together with genome sequence information and its annotation. The 6,650,282 bp high-quality permanent draft genome is arranged into 91 scaffolds of 91 contigs containing 6,427 protein-coding genes and 68 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0119-5) contains supplementary material, which is available to authorized users. BioMed Central 2015-12-10 /pmc/articles/PMC4674904/ /pubmed/26664655 http://dx.doi.org/10.1186/s40793-015-0119-5 Text en © Ardley et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Genome Report
Ardley, Julie
Tian, Rui
O’Hara, Graham
Seshadri, Rekha
Reddy, T. B. K.
Pati, Amrita
Woyke, Tanja
Markowitz, Victor
Ivanova, Natalia
Kyrpides, Nikos
Howieson, John
Reeve, Wayne
High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil
title High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil
title_full High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil
title_fullStr High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil
title_full_unstemmed High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil
title_short High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil
title_sort high-quality permanent draft genome sequence of ensifer medicae strain wsm244, a microsymbiont isolated from medicago polymorpha growing in alkaline soil
topic Short Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674904/
https://www.ncbi.nlm.nih.gov/pubmed/26664655
http://dx.doi.org/10.1186/s40793-015-0119-5
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