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Silica Nanowires Templated by Amyloid-like Fibrils

Many peptides self-assemble to form amyloid fibrils. We previously explored the sequence propensity to form amyloid using variants of a designed peptide with sequence KFFEAAAKKFFE. These variant peptides form highly stable amyloid fibrils with varied lateral assembly and are ideal to template furthe...

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Detalles Bibliográficos
Autores principales: Al-Garawi, Zahraa S, Thorpe, Julian R, Serpell, Louise C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674975/
https://www.ncbi.nlm.nih.gov/pubmed/26434656
http://dx.doi.org/10.1002/anie.201508415
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author Al-Garawi, Zahraa S
Thorpe, Julian R
Serpell, Louise C
author_facet Al-Garawi, Zahraa S
Thorpe, Julian R
Serpell, Louise C
author_sort Al-Garawi, Zahraa S
collection PubMed
description Many peptides self-assemble to form amyloid fibrils. We previously explored the sequence propensity to form amyloid using variants of a designed peptide with sequence KFFEAAAKKFFE. These variant peptides form highly stable amyloid fibrils with varied lateral assembly and are ideal to template further assembly of non-proteinaceous material. Herein, we show that the fibrils formed by peptide variants can be coated with a layer of silica to produce silica nanowires using tetraethyl-orthosilicate. The resulting nanowires were characterized using electron microscopy (TEM), X-ray fiber diffraction, FTIR and cross-section EM to reveal a nanostructure with peptidic core. Lysine residues play a role in templating the formation of silica on the fibril surface and, using this library of peptides, we have explored the contributions of lysine as well as arginine to silica templating, and find that sequence plays an important role in determining the physical nature and structure of the resulting nanowires.
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spelling pubmed-46749752015-12-18 Silica Nanowires Templated by Amyloid-like Fibrils Al-Garawi, Zahraa S Thorpe, Julian R Serpell, Louise C Angew Chem Int Ed Engl Communications Many peptides self-assemble to form amyloid fibrils. We previously explored the sequence propensity to form amyloid using variants of a designed peptide with sequence KFFEAAAKKFFE. These variant peptides form highly stable amyloid fibrils with varied lateral assembly and are ideal to template further assembly of non-proteinaceous material. Herein, we show that the fibrils formed by peptide variants can be coated with a layer of silica to produce silica nanowires using tetraethyl-orthosilicate. The resulting nanowires were characterized using electron microscopy (TEM), X-ray fiber diffraction, FTIR and cross-section EM to reveal a nanostructure with peptidic core. Lysine residues play a role in templating the formation of silica on the fibril surface and, using this library of peptides, we have explored the contributions of lysine as well as arginine to silica templating, and find that sequence plays an important role in determining the physical nature and structure of the resulting nanowires. WILEY-VCH Verlag 2015-11-02 2015-10-05 /pmc/articles/PMC4674975/ /pubmed/26434656 http://dx.doi.org/10.1002/anie.201508415 Text en © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/ © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Al-Garawi, Zahraa S
Thorpe, Julian R
Serpell, Louise C
Silica Nanowires Templated by Amyloid-like Fibrils
title Silica Nanowires Templated by Amyloid-like Fibrils
title_full Silica Nanowires Templated by Amyloid-like Fibrils
title_fullStr Silica Nanowires Templated by Amyloid-like Fibrils
title_full_unstemmed Silica Nanowires Templated by Amyloid-like Fibrils
title_short Silica Nanowires Templated by Amyloid-like Fibrils
title_sort silica nanowires templated by amyloid-like fibrils
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674975/
https://www.ncbi.nlm.nih.gov/pubmed/26434656
http://dx.doi.org/10.1002/anie.201508415
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