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The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China

BACKGROUND: Rickettsia spp. belonging to the spotted fever group (SFG) cause infections in humans, domestic animals and wildlife. At least five SFG rickettsial species have been reported in China, but the occurrence of Rickettsia aeschlimannii and R. massiliae in ticks has not been characterized to...

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Autores principales: Wei, Qing-Qing, Guo, Li-Ping, Wang, An-Dong, Mu, Lu-Meng, Zhang, Ke, Chen, Chuang-Fu, Zhang, Wan-Jiang, Wang, Yuan-Zhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675064/
https://www.ncbi.nlm.nih.gov/pubmed/26652857
http://dx.doi.org/10.1186/s13071-015-1242-2
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author Wei, Qing-Qing
Guo, Li-Ping
Wang, An-Dong
Mu, Lu-Meng
Zhang, Ke
Chen, Chuang-Fu
Zhang, Wan-Jiang
Wang, Yuan-Zhi
author_facet Wei, Qing-Qing
Guo, Li-Ping
Wang, An-Dong
Mu, Lu-Meng
Zhang, Ke
Chen, Chuang-Fu
Zhang, Wan-Jiang
Wang, Yuan-Zhi
author_sort Wei, Qing-Qing
collection PubMed
description BACKGROUND: Rickettsia spp. belonging to the spotted fever group (SFG) cause infections in humans, domestic animals and wildlife. At least five SFG rickettsial species have been reported in China, but the occurrence of Rickettsia aeschlimannii and R. massiliae in ticks has not been characterized to date. FINDINGS: A total of 114 adult ticks were collected from sheep in Yining County, Xinjiang Uygur Autonomous Region, in northwest China. The ticks were identified from morphological and molecular characteristics. All samples were examined by polymerase chain reaction (PCR), and six genetic markers were used to determine the Rickettsia spp. in the ticks. The ticks collected were identified as Rhipicephalus turanicus. Three different lineages of Rh. turanicus from Yining County were discovered on phylogenetic analysis of 16S rDNA and cox1. Twenty-one of the 114 samples (18.42%) were positive for rickettsial agents. Phylogenetic analysis based on six genetic sequences showed that three rickettsial species were present, namely: R. aeschlimannii (19.05%, 4/21), R. massiliae (19.05%, 4/21) and R. sibirica variant (61.90%, 13/21), which is clustered in the clade of R. sibirica subsp. sibirica. CONCLUSIONS: This is the first description of R. aeschlimannii and R. massiliae in China. R. massiliae, R. aeschlimannii and R. sibirica variant co-circulate in the region of the China-Kazakhstan border, in northwest China. Rickettsial agents in ticks of the genus Rhipicephalus from migrant birds, transported livestock, wildlife and human beings should be investigated further in the region of the China–Central Asian border. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-1242-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-46750642015-12-11 The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China Wei, Qing-Qing Guo, Li-Ping Wang, An-Dong Mu, Lu-Meng Zhang, Ke Chen, Chuang-Fu Zhang, Wan-Jiang Wang, Yuan-Zhi Parasit Vectors Short Report BACKGROUND: Rickettsia spp. belonging to the spotted fever group (SFG) cause infections in humans, domestic animals and wildlife. At least five SFG rickettsial species have been reported in China, but the occurrence of Rickettsia aeschlimannii and R. massiliae in ticks has not been characterized to date. FINDINGS: A total of 114 adult ticks were collected from sheep in Yining County, Xinjiang Uygur Autonomous Region, in northwest China. The ticks were identified from morphological and molecular characteristics. All samples were examined by polymerase chain reaction (PCR), and six genetic markers were used to determine the Rickettsia spp. in the ticks. The ticks collected were identified as Rhipicephalus turanicus. Three different lineages of Rh. turanicus from Yining County were discovered on phylogenetic analysis of 16S rDNA and cox1. Twenty-one of the 114 samples (18.42%) were positive for rickettsial agents. Phylogenetic analysis based on six genetic sequences showed that three rickettsial species were present, namely: R. aeschlimannii (19.05%, 4/21), R. massiliae (19.05%, 4/21) and R. sibirica variant (61.90%, 13/21), which is clustered in the clade of R. sibirica subsp. sibirica. CONCLUSIONS: This is the first description of R. aeschlimannii and R. massiliae in China. R. massiliae, R. aeschlimannii and R. sibirica variant co-circulate in the region of the China-Kazakhstan border, in northwest China. Rickettsial agents in ticks of the genus Rhipicephalus from migrant birds, transported livestock, wildlife and human beings should be investigated further in the region of the China–Central Asian border. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-1242-2) contains supplementary material, which is available to authorized users. BioMed Central 2015-12-10 /pmc/articles/PMC4675064/ /pubmed/26652857 http://dx.doi.org/10.1186/s13071-015-1242-2 Text en © Wei et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Wei, Qing-Qing
Guo, Li-Ping
Wang, An-Dong
Mu, Lu-Meng
Zhang, Ke
Chen, Chuang-Fu
Zhang, Wan-Jiang
Wang, Yuan-Zhi
The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China
title The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China
title_full The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China
title_fullStr The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China
title_full_unstemmed The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China
title_short The first detection of Rickettsia aeschlimannii and Rickettsia massiliae in Rhipicephalus turanicus ticks, in northwest China
title_sort first detection of rickettsia aeschlimannii and rickettsia massiliae in rhipicephalus turanicus ticks, in northwest china
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675064/
https://www.ncbi.nlm.nih.gov/pubmed/26652857
http://dx.doi.org/10.1186/s13071-015-1242-2
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