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Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis

PURPOSE: Meningitis is among the most common infections affecting the central nervous system. It can be difficult to determine the exact pathogen responsible for the infection and patients are often treated with empiric antibiotics. This study was conducted to identify the most common clinical chara...

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Autores principales: Jin, Dahee, Heo, Tae Hoon, Byeon, Jung Hye, Kim, Gun-Ha, Kim, Mi Kyung, Eun, So-Hee, Eun, Baik-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Pediatric Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675926/
https://www.ncbi.nlm.nih.gov/pubmed/26692881
http://dx.doi.org/10.3345/kjp.2015.58.11.446
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author Jin, Dahee
Heo, Tae Hoon
Byeon, Jung Hye
Kim, Gun-Ha
Kim, Mi Kyung
Eun, So-Hee
Eun, Baik-Lin
author_facet Jin, Dahee
Heo, Tae Hoon
Byeon, Jung Hye
Kim, Gun-Ha
Kim, Mi Kyung
Eun, So-Hee
Eun, Baik-Lin
author_sort Jin, Dahee
collection PubMed
description PURPOSE: Meningitis is among the most common infections affecting the central nervous system. It can be difficult to determine the exact pathogen responsible for the infection and patients are often treated with empiric antibiotics. This study was conducted to identify the most common clinical characteristics of enteroviral meningitis in children and evaluate the diagnostic efficacy of reverse transcriptase-polymerase chain reaction (RT-PCR) for early detection of an enterovirus. METHODS: We analyzed the medical records of children admitted to Korea University Medical Center and diagnosed with meningitis on the basis of cerebrospinal fluid (CSF) analysis and RT-PCR from CSF and other samples from January 2010 to August 2013. RESULTS: A total of 333 patients were enrolled and classified into four groups based on diagnosis: enteroviral meningitis (n=110), bacterial meningitis (n=23), other viral meningitis (n=36), and unknown etiology (n=164). Patients with bacterial meningitis were younger than those in the other groups (P<0.001). Pleocytosis in CSF was similar across all groups. Of patients in the enteroviral meningitis group, 92.7% were diagnosed based on RT-PCR findings. Mean length of hospital stay for patients with enteroviral meningitis was 6.08 days, which was significantly shorter than that for patients with meningitis of bacterial etiology (19.73 days, P<0.001). CONCLUSION: Diagnosis of enteroviral meningitis before viral culture results are available is possible using RT-PCR. Accurate diagnosis reduces the length of hospital stay and helps to avoid unnecessary empiric antibiotic treatment.
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spelling pubmed-46759262015-12-11 Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis Jin, Dahee Heo, Tae Hoon Byeon, Jung Hye Kim, Gun-Ha Kim, Mi Kyung Eun, So-Hee Eun, Baik-Lin Korean J Pediatr Original Article PURPOSE: Meningitis is among the most common infections affecting the central nervous system. It can be difficult to determine the exact pathogen responsible for the infection and patients are often treated with empiric antibiotics. This study was conducted to identify the most common clinical characteristics of enteroviral meningitis in children and evaluate the diagnostic efficacy of reverse transcriptase-polymerase chain reaction (RT-PCR) for early detection of an enterovirus. METHODS: We analyzed the medical records of children admitted to Korea University Medical Center and diagnosed with meningitis on the basis of cerebrospinal fluid (CSF) analysis and RT-PCR from CSF and other samples from January 2010 to August 2013. RESULTS: A total of 333 patients were enrolled and classified into four groups based on diagnosis: enteroviral meningitis (n=110), bacterial meningitis (n=23), other viral meningitis (n=36), and unknown etiology (n=164). Patients with bacterial meningitis were younger than those in the other groups (P<0.001). Pleocytosis in CSF was similar across all groups. Of patients in the enteroviral meningitis group, 92.7% were diagnosed based on RT-PCR findings. Mean length of hospital stay for patients with enteroviral meningitis was 6.08 days, which was significantly shorter than that for patients with meningitis of bacterial etiology (19.73 days, P<0.001). CONCLUSION: Diagnosis of enteroviral meningitis before viral culture results are available is possible using RT-PCR. Accurate diagnosis reduces the length of hospital stay and helps to avoid unnecessary empiric antibiotic treatment. The Korean Pediatric Society 2015-11 2015-11-22 /pmc/articles/PMC4675926/ /pubmed/26692881 http://dx.doi.org/10.3345/kjp.2015.58.11.446 Text en Copyright © 2015 by The Korean Pediatric Society http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jin, Dahee
Heo, Tae Hoon
Byeon, Jung Hye
Kim, Gun-Ha
Kim, Mi Kyung
Eun, So-Hee
Eun, Baik-Lin
Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis
title Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis
title_full Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis
title_fullStr Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis
title_full_unstemmed Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis
title_short Analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis
title_sort analysis of clinical information and reverse transcriptase-polymerase chain reaction for early diagnosis of enteroviral meningitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675926/
https://www.ncbi.nlm.nih.gov/pubmed/26692881
http://dx.doi.org/10.3345/kjp.2015.58.11.446
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