Cargando…

EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE

BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sepsis METHODS: Several ESBL inoculums r...

Descripción completa

Detalles Bibliográficos
Autores principales: TOLEDO, Paula Virginia Michelon, TUON, Felipe Francisco, BAIL, Larissa, MANENTE, Francine, ARRUDA, Polliane, ARANHA-JUNIOR, Ayrton Alves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Cirurgia Digestiva 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676372/
https://www.ncbi.nlm.nih.gov/pubmed/25184764
http://dx.doi.org/10.1590/S0102-67202014000300002
_version_ 1782405168453648384
author TOLEDO, Paula Virginia Michelon
TUON, Felipe Francisco
BAIL, Larissa
MANENTE, Francine
ARRUDA, Polliane
ARANHA-JUNIOR, Ayrton Alves
author_facet TOLEDO, Paula Virginia Michelon
TUON, Felipe Francisco
BAIL, Larissa
MANENTE, Francine
ARRUDA, Polliane
ARANHA-JUNIOR, Ayrton Alves
author_sort TOLEDO, Paula Virginia Michelon
collection PubMed
description BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sepsis METHODS: Several ESBL inoculums ranging from 1.5x10(9) colony-forming units per milliliter (CFU/mL) to 2.0x10(10)CFU/mL were administered by peritoneal injection in adults Wistar rats. Outcomes and microbiological data of quantitative peritoneal and blood cultures were observed in untreated animals. Animals which received 2.0x10(10)CFU/mL inoculums were treated with single meropenem dose (30mg/kg) after one hour and those which received 1.0x10(10)CFU/mL inoculums were treated immediately with three doses of meropenem 50 mg/kg. Outcomes were observed for 24 hours after inoculation. RESULTS: Solutions with 1.5 x10(9) and 6.0x10(9) CFU/mL were not lethal within 24 hours. Inoculums of 1.0x10(10)CFU/mL were lethal in 80% and solutions with 2.0x10(10) CFU/mL were lethal in 100% of animals. ESBL lethal sepsis (1.0x10(10)CFU/mL) was treated immediately with 50 mg/kg of meropenem every eight hours for 24 hours and presented 40% mortality compared with 80% mortality of the control group (p=0.033). Quantitative cultures of peritoneal fluid presented 10(4)CFU/mL or less for treated animals compared to more than 10(5) for untreated animals (p=0.001). CONCLUSION: Inoculums of 1.0x10(10)CFU/mL achieved the best results to study a model of lethal sepsis and this model of treatment of carbapenem-susceptible Enterobacteriaceae can serve as control to further evaluation of treatment of carbapenemase-producing Enterobacteriaceae models.
format Online
Article
Text
id pubmed-4676372
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Colégio Brasileiro de Cirurgia Digestiva
record_format MEDLINE/PubMed
spelling pubmed-46763722016-02-24 EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE TOLEDO, Paula Virginia Michelon TUON, Felipe Francisco BAIL, Larissa MANENTE, Francine ARRUDA, Polliane ARANHA-JUNIOR, Ayrton Alves Arq Bras Cir Dig Original Article BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sepsis METHODS: Several ESBL inoculums ranging from 1.5x10(9) colony-forming units per milliliter (CFU/mL) to 2.0x10(10)CFU/mL were administered by peritoneal injection in adults Wistar rats. Outcomes and microbiological data of quantitative peritoneal and blood cultures were observed in untreated animals. Animals which received 2.0x10(10)CFU/mL inoculums were treated with single meropenem dose (30mg/kg) after one hour and those which received 1.0x10(10)CFU/mL inoculums were treated immediately with three doses of meropenem 50 mg/kg. Outcomes were observed for 24 hours after inoculation. RESULTS: Solutions with 1.5 x10(9) and 6.0x10(9) CFU/mL were not lethal within 24 hours. Inoculums of 1.0x10(10)CFU/mL were lethal in 80% and solutions with 2.0x10(10) CFU/mL were lethal in 100% of animals. ESBL lethal sepsis (1.0x10(10)CFU/mL) was treated immediately with 50 mg/kg of meropenem every eight hours for 24 hours and presented 40% mortality compared with 80% mortality of the control group (p=0.033). Quantitative cultures of peritoneal fluid presented 10(4)CFU/mL or less for treated animals compared to more than 10(5) for untreated animals (p=0.001). CONCLUSION: Inoculums of 1.0x10(10)CFU/mL achieved the best results to study a model of lethal sepsis and this model of treatment of carbapenem-susceptible Enterobacteriaceae can serve as control to further evaluation of treatment of carbapenemase-producing Enterobacteriaceae models. Colégio Brasileiro de Cirurgia Digestiva 2014 /pmc/articles/PMC4676372/ /pubmed/25184764 http://dx.doi.org/10.1590/S0102-67202014000300002 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
TOLEDO, Paula Virginia Michelon
TUON, Felipe Francisco
BAIL, Larissa
MANENTE, Francine
ARRUDA, Polliane
ARANHA-JUNIOR, Ayrton Alves
EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE
title EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE
title_full EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE
title_fullStr EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE
title_full_unstemmed EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE
title_short EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE
title_sort experimental model for treatment of extended spectrum betalactamase producing-klebsiella pneumoniae
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676372/
https://www.ncbi.nlm.nih.gov/pubmed/25184764
http://dx.doi.org/10.1590/S0102-67202014000300002
work_keys_str_mv AT toledopaulavirginiamichelon experimentalmodelfortreatmentofextendedspectrumbetalactamaseproducingklebsiellapneumoniae
AT tuonfelipefrancisco experimentalmodelfortreatmentofextendedspectrumbetalactamaseproducingklebsiellapneumoniae
AT baillarissa experimentalmodelfortreatmentofextendedspectrumbetalactamaseproducingklebsiellapneumoniae
AT manentefrancine experimentalmodelfortreatmentofextendedspectrumbetalactamaseproducingklebsiellapneumoniae
AT arrudapolliane experimentalmodelfortreatmentofextendedspectrumbetalactamaseproducingklebsiellapneumoniae
AT aranhajuniorayrtonalves experimentalmodelfortreatmentofextendedspectrumbetalactamaseproducingklebsiellapneumoniae