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EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE
BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sepsis METHODS: Several ESBL inoculums r...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Cirurgia Digestiva
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676372/ https://www.ncbi.nlm.nih.gov/pubmed/25184764 http://dx.doi.org/10.1590/S0102-67202014000300002 |
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author | TOLEDO, Paula Virginia Michelon TUON, Felipe Francisco BAIL, Larissa MANENTE, Francine ARRUDA, Polliane ARANHA-JUNIOR, Ayrton Alves |
author_facet | TOLEDO, Paula Virginia Michelon TUON, Felipe Francisco BAIL, Larissa MANENTE, Francine ARRUDA, Polliane ARANHA-JUNIOR, Ayrton Alves |
author_sort | TOLEDO, Paula Virginia Michelon |
collection | PubMed |
description | BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sepsis METHODS: Several ESBL inoculums ranging from 1.5x10(9) colony-forming units per milliliter (CFU/mL) to 2.0x10(10)CFU/mL were administered by peritoneal injection in adults Wistar rats. Outcomes and microbiological data of quantitative peritoneal and blood cultures were observed in untreated animals. Animals which received 2.0x10(10)CFU/mL inoculums were treated with single meropenem dose (30mg/kg) after one hour and those which received 1.0x10(10)CFU/mL inoculums were treated immediately with three doses of meropenem 50 mg/kg. Outcomes were observed for 24 hours after inoculation. RESULTS: Solutions with 1.5 x10(9) and 6.0x10(9) CFU/mL were not lethal within 24 hours. Inoculums of 1.0x10(10)CFU/mL were lethal in 80% and solutions with 2.0x10(10) CFU/mL were lethal in 100% of animals. ESBL lethal sepsis (1.0x10(10)CFU/mL) was treated immediately with 50 mg/kg of meropenem every eight hours for 24 hours and presented 40% mortality compared with 80% mortality of the control group (p=0.033). Quantitative cultures of peritoneal fluid presented 10(4)CFU/mL or less for treated animals compared to more than 10(5) for untreated animals (p=0.001). CONCLUSION: Inoculums of 1.0x10(10)CFU/mL achieved the best results to study a model of lethal sepsis and this model of treatment of carbapenem-susceptible Enterobacteriaceae can serve as control to further evaluation of treatment of carbapenemase-producing Enterobacteriaceae models. |
format | Online Article Text |
id | pubmed-4676372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Colégio Brasileiro de Cirurgia Digestiva |
record_format | MEDLINE/PubMed |
spelling | pubmed-46763722016-02-24 EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE TOLEDO, Paula Virginia Michelon TUON, Felipe Francisco BAIL, Larissa MANENTE, Francine ARRUDA, Polliane ARANHA-JUNIOR, Ayrton Alves Arq Bras Cir Dig Original Article BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sepsis METHODS: Several ESBL inoculums ranging from 1.5x10(9) colony-forming units per milliliter (CFU/mL) to 2.0x10(10)CFU/mL were administered by peritoneal injection in adults Wistar rats. Outcomes and microbiological data of quantitative peritoneal and blood cultures were observed in untreated animals. Animals which received 2.0x10(10)CFU/mL inoculums were treated with single meropenem dose (30mg/kg) after one hour and those which received 1.0x10(10)CFU/mL inoculums were treated immediately with three doses of meropenem 50 mg/kg. Outcomes were observed for 24 hours after inoculation. RESULTS: Solutions with 1.5 x10(9) and 6.0x10(9) CFU/mL were not lethal within 24 hours. Inoculums of 1.0x10(10)CFU/mL were lethal in 80% and solutions with 2.0x10(10) CFU/mL were lethal in 100% of animals. ESBL lethal sepsis (1.0x10(10)CFU/mL) was treated immediately with 50 mg/kg of meropenem every eight hours for 24 hours and presented 40% mortality compared with 80% mortality of the control group (p=0.033). Quantitative cultures of peritoneal fluid presented 10(4)CFU/mL or less for treated animals compared to more than 10(5) for untreated animals (p=0.001). CONCLUSION: Inoculums of 1.0x10(10)CFU/mL achieved the best results to study a model of lethal sepsis and this model of treatment of carbapenem-susceptible Enterobacteriaceae can serve as control to further evaluation of treatment of carbapenemase-producing Enterobacteriaceae models. Colégio Brasileiro de Cirurgia Digestiva 2014 /pmc/articles/PMC4676372/ /pubmed/25184764 http://dx.doi.org/10.1590/S0102-67202014000300002 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article TOLEDO, Paula Virginia Michelon TUON, Felipe Francisco BAIL, Larissa MANENTE, Francine ARRUDA, Polliane ARANHA-JUNIOR, Ayrton Alves EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE PRODUCING-KLEBSIELLA PNEUMONIAE |
title | EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE
PRODUCING-KLEBSIELLA PNEUMONIAE |
title_full | EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE
PRODUCING-KLEBSIELLA PNEUMONIAE |
title_fullStr | EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE
PRODUCING-KLEBSIELLA PNEUMONIAE |
title_full_unstemmed | EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE
PRODUCING-KLEBSIELLA PNEUMONIAE |
title_short | EXPERIMENTAL MODEL FOR TREATMENT OF EXTENDED SPECTRUM BETALACTAMASE
PRODUCING-KLEBSIELLA PNEUMONIAE |
title_sort | experimental model for treatment of extended spectrum betalactamase
producing-klebsiella pneumoniae |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676372/ https://www.ncbi.nlm.nih.gov/pubmed/25184764 http://dx.doi.org/10.1590/S0102-67202014000300002 |
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