Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge

The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge...

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Autores principales: Hirakata, Yuga, Oshiki, Mamoru, Kuroda, Kyohei, Hatamoto, Masashi, Kubota, Kengo, Yamaguchi, Takashi, Harada, Hideki, Araki, Nobuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676557/
https://www.ncbi.nlm.nih.gov/pubmed/26639580
http://dx.doi.org/10.1264/jsme2.ME15154
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author Hirakata, Yuga
Oshiki, Mamoru
Kuroda, Kyohei
Hatamoto, Masashi
Kubota, Kengo
Yamaguchi, Takashi
Harada, Hideki
Araki, Nobuo
author_facet Hirakata, Yuga
Oshiki, Mamoru
Kuroda, Kyohei
Hatamoto, Masashi
Kubota, Kengo
Yamaguchi, Takashi
Harada, Hideki
Araki, Nobuo
author_sort Hirakata, Yuga
collection PubMed
description The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates.
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spelling pubmed-46765572015-12-21 Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge Hirakata, Yuga Oshiki, Mamoru Kuroda, Kyohei Hatamoto, Masashi Kubota, Kengo Yamaguchi, Takashi Harada, Hideki Araki, Nobuo Microbes Environ Short Communication The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2015-12 2015-12-04 /pmc/articles/PMC4676557/ /pubmed/26639580 http://dx.doi.org/10.1264/jsme2.ME15154 Text en Copyright © 2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Hirakata, Yuga
Oshiki, Mamoru
Kuroda, Kyohei
Hatamoto, Masashi
Kubota, Kengo
Yamaguchi, Takashi
Harada, Hideki
Araki, Nobuo
Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge
title Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge
title_full Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge
title_fullStr Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge
title_full_unstemmed Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge
title_short Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge
title_sort identification and detection of prokaryotic symbionts in the ciliate metopus from anaerobic granular sludge
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676557/
https://www.ncbi.nlm.nih.gov/pubmed/26639580
http://dx.doi.org/10.1264/jsme2.ME15154
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