Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline

The persistence of the multi-drug resistance plasmids pAQU1 and IncFIB was examined in bacterial populations under very low selective pressure. We herein demonstrated that these plasmids stably remained not only in the original host, but also in a transconjugant, even after being in a non-culturable...

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Autores principales: Bien, Thi Lan Thanh, Sato-Takabe, Yuki, Ogo, Mitsuko, Usui, Masaru, Suzuki, Satoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676558/
https://www.ncbi.nlm.nih.gov/pubmed/26639579
http://dx.doi.org/10.1264/jsme2.ME15122
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author Bien, Thi Lan Thanh
Sato-Takabe, Yuki
Ogo, Mitsuko
Usui, Masaru
Suzuki, Satoru
author_facet Bien, Thi Lan Thanh
Sato-Takabe, Yuki
Ogo, Mitsuko
Usui, Masaru
Suzuki, Satoru
author_sort Bien, Thi Lan Thanh
collection PubMed
description The persistence of the multi-drug resistance plasmids pAQU1 and IncFIB was examined in bacterial populations under very low selective pressure. We herein demonstrated that these plasmids stably remained not only in the original host, but also in a transconjugant, even after being in a non-culturable state. In seawater microcosms containing Photobacterium damselae 04Ya311 possessing pAQU1, no significant loss of pAQU1 was observed during a 30-d starvation period. The copy numbers of pAQU1 and IncFIB in E. coli were constant. The results of the present study suggest that these plasmids have the ability to remain among various bacteria under oligotrophic conditions with low antibiotic selection pressure.
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spelling pubmed-46765582015-12-21 Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline Bien, Thi Lan Thanh Sato-Takabe, Yuki Ogo, Mitsuko Usui, Masaru Suzuki, Satoru Microbes Environ Short Communication The persistence of the multi-drug resistance plasmids pAQU1 and IncFIB was examined in bacterial populations under very low selective pressure. We herein demonstrated that these plasmids stably remained not only in the original host, but also in a transconjugant, even after being in a non-culturable state. In seawater microcosms containing Photobacterium damselae 04Ya311 possessing pAQU1, no significant loss of pAQU1 was observed during a 30-d starvation period. The copy numbers of pAQU1 and IncFIB in E. coli were constant. The results of the present study suggest that these plasmids have the ability to remain among various bacteria under oligotrophic conditions with low antibiotic selection pressure. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2015-12 2015-12-04 /pmc/articles/PMC4676558/ /pubmed/26639579 http://dx.doi.org/10.1264/jsme2.ME15122 Text en Copyright © 2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Bien, Thi Lan Thanh
Sato-Takabe, Yuki
Ogo, Mitsuko
Usui, Masaru
Suzuki, Satoru
Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline
title Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline
title_full Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline
title_fullStr Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline
title_full_unstemmed Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline
title_short Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline
title_sort persistence of multi-drug resistance plasmids in sterile water under very low concentrations of tetracycline
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676558/
https://www.ncbi.nlm.nih.gov/pubmed/26639579
http://dx.doi.org/10.1264/jsme2.ME15122
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