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Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba

Giardia duodenalis is considered the most common protozoan infecting humans worldwide. Molecular characterization of G. duodenalis isolates has revealed the existence of eight groups (assemblages A to H) which differ in their host distribution. A cross-sectional study was conducted in 639 children f...

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Autores principales: Jerez Puebla, Luis Enrique, Núñez, Fidel A., Martínez Silva, Isabel, Rojas Rivero, Lázara, Martínez González, Marta, Méndez Sutil, Yuliet, Ayllón Valdés, Lucía, Atencio Millán, Iraís, Müller, Norbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4677018/
https://www.ncbi.nlm.nih.gov/pubmed/26693345
http://dx.doi.org/10.1155/2015/378643
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author Jerez Puebla, Luis Enrique
Núñez, Fidel A.
Martínez Silva, Isabel
Rojas Rivero, Lázara
Martínez González, Marta
Méndez Sutil, Yuliet
Ayllón Valdés, Lucía
Atencio Millán, Iraís
Müller, Norbert
author_facet Jerez Puebla, Luis Enrique
Núñez, Fidel A.
Martínez Silva, Isabel
Rojas Rivero, Lázara
Martínez González, Marta
Méndez Sutil, Yuliet
Ayllón Valdés, Lucía
Atencio Millán, Iraís
Müller, Norbert
author_sort Jerez Puebla, Luis Enrique
collection PubMed
description Giardia duodenalis is considered the most common protozoan infecting humans worldwide. Molecular characterization of G. duodenalis isolates has revealed the existence of eight groups (assemblages A to H) which differ in their host distribution. A cross-sectional study was conducted in 639 children from La Habana between January and December 2013. Two assemblage-specific PCRs were carried out for the molecular characterization. The overall prevalence of Giardia infection was 11.9%. DNA from 63 of 76 (82.9%) samples was successfully amplified by PCR-tpi, while 58 from 76 (76.3%) were detected by PCRE1-HF. Similar results by both PCRs were obtained in 54 from 76 samples (71%). According to these analyses, assemblage B and mixed assemblages A + B account for most of the Giardia infections in the cohort of children tested. Our current study identified assemblage B as predominant genotype in children infected with Giardia. Univariate analysis indicated that omission of washing hands before eating and keeping dogs at home were significant risk factors for a Giardia infection. In the future, novel molecular tools for a better discrimination of assemblages at the subassemblages level are needed to verify possible correlations between Giardia genotypes and symptomatology of giardiasis.
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spelling pubmed-46770182015-12-21 Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba Jerez Puebla, Luis Enrique Núñez, Fidel A. Martínez Silva, Isabel Rojas Rivero, Lázara Martínez González, Marta Méndez Sutil, Yuliet Ayllón Valdés, Lucía Atencio Millán, Iraís Müller, Norbert J Parasitol Res Research Article Giardia duodenalis is considered the most common protozoan infecting humans worldwide. Molecular characterization of G. duodenalis isolates has revealed the existence of eight groups (assemblages A to H) which differ in their host distribution. A cross-sectional study was conducted in 639 children from La Habana between January and December 2013. Two assemblage-specific PCRs were carried out for the molecular characterization. The overall prevalence of Giardia infection was 11.9%. DNA from 63 of 76 (82.9%) samples was successfully amplified by PCR-tpi, while 58 from 76 (76.3%) were detected by PCRE1-HF. Similar results by both PCRs were obtained in 54 from 76 samples (71%). According to these analyses, assemblage B and mixed assemblages A + B account for most of the Giardia infections in the cohort of children tested. Our current study identified assemblage B as predominant genotype in children infected with Giardia. Univariate analysis indicated that omission of washing hands before eating and keeping dogs at home were significant risk factors for a Giardia infection. In the future, novel molecular tools for a better discrimination of assemblages at the subassemblages level are needed to verify possible correlations between Giardia genotypes and symptomatology of giardiasis. Hindawi Publishing Corporation 2015 2015-11-29 /pmc/articles/PMC4677018/ /pubmed/26693345 http://dx.doi.org/10.1155/2015/378643 Text en Copyright © 2015 Luis Enrique Jerez Puebla et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jerez Puebla, Luis Enrique
Núñez, Fidel A.
Martínez Silva, Isabel
Rojas Rivero, Lázara
Martínez González, Marta
Méndez Sutil, Yuliet
Ayllón Valdés, Lucía
Atencio Millán, Iraís
Müller, Norbert
Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba
title Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba
title_full Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba
title_fullStr Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba
title_full_unstemmed Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba
title_short Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba
title_sort molecular characterization and risk factors of giardia duodenalis among school children from la habana, cuba
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4677018/
https://www.ncbi.nlm.nih.gov/pubmed/26693345
http://dx.doi.org/10.1155/2015/378643
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