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Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B

BACKGROUND: Serum hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) liver function in patients with chronic hepatitis B (CHB) are significantly associated. A comparison of clinical significance of fecal HBV DNA and serum HBV DNA has not yet been reported. MATERIAL/METHODS: Stool and seru...

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Autores principales: Zheng, Ji-shun, Chen, Meng-meng, Yang, Hai-fei, Zhou, Xiang-tian, Liu, Yan-yan, Li, Jia-bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4677737/
https://www.ncbi.nlm.nih.gov/pubmed/26645150
http://dx.doi.org/10.12659/MSM.896243
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author Zheng, Ji-shun
Chen, Meng-meng
Yang, Hai-fei
Zhou, Xiang-tian
Liu, Yan-yan
Li, Jia-bin
author_facet Zheng, Ji-shun
Chen, Meng-meng
Yang, Hai-fei
Zhou, Xiang-tian
Liu, Yan-yan
Li, Jia-bin
author_sort Zheng, Ji-shun
collection PubMed
description BACKGROUND: Serum hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) liver function in patients with chronic hepatitis B (CHB) are significantly associated. A comparison of clinical significance of fecal HBV DNA and serum HBV DNA has not yet been reported. MATERIAL/METHODS: Stool and serum samples were collected from 66 patients with CHB. Fecal HBV DNA, serum HBV DNA, and intestinal microbiota DNA were detected by real-time quantitative fluorescence polymerase chain reaction (PCR). Liver function and HBeAg were analyzed. RESULTS: The stool and serum HBV DNA were positively correlated (r=0.57, P=0.001). Fecal HBV DNA was higher in the HBeAg-positive group than in the HBeAg-negative group (P=0.02). Fecal HBV DNA was negatively correlated with alkaline phosphatase (ALP) (r=−0.41, P=0.001) and TBIL (r=−0.29, P=0.02), and was positively correlated with Enterococcus (r=0.38, P=0.002). Serum HBV DNA was negatively correlated with alanine aminotransferase (ALT) (r=−0.30, P=0.02), aminotransferase (AST) (r=−0.26, P=0.049), and Lactobacillus (r=−0.31, P=0.01). CONCLUSIONS: These observations suggest that fecal HBV DNA and serum HBV DNA in patients with CHB have different effects. Fecal HBV DNA might be associated with changes in Enterococcus concentrations, but serum HBV DNA is not.
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spelling pubmed-46777372015-12-18 Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B Zheng, Ji-shun Chen, Meng-meng Yang, Hai-fei Zhou, Xiang-tian Liu, Yan-yan Li, Jia-bin Med Sci Monit Clinical Research BACKGROUND: Serum hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) liver function in patients with chronic hepatitis B (CHB) are significantly associated. A comparison of clinical significance of fecal HBV DNA and serum HBV DNA has not yet been reported. MATERIAL/METHODS: Stool and serum samples were collected from 66 patients with CHB. Fecal HBV DNA, serum HBV DNA, and intestinal microbiota DNA were detected by real-time quantitative fluorescence polymerase chain reaction (PCR). Liver function and HBeAg were analyzed. RESULTS: The stool and serum HBV DNA were positively correlated (r=0.57, P=0.001). Fecal HBV DNA was higher in the HBeAg-positive group than in the HBeAg-negative group (P=0.02). Fecal HBV DNA was negatively correlated with alkaline phosphatase (ALP) (r=−0.41, P=0.001) and TBIL (r=−0.29, P=0.02), and was positively correlated with Enterococcus (r=0.38, P=0.002). Serum HBV DNA was negatively correlated with alanine aminotransferase (ALT) (r=−0.30, P=0.02), aminotransferase (AST) (r=−0.26, P=0.049), and Lactobacillus (r=−0.31, P=0.01). CONCLUSIONS: These observations suggest that fecal HBV DNA and serum HBV DNA in patients with CHB have different effects. Fecal HBV DNA might be associated with changes in Enterococcus concentrations, but serum HBV DNA is not. International Scientific Literature, Inc. 2015-12-09 /pmc/articles/PMC4677737/ /pubmed/26645150 http://dx.doi.org/10.12659/MSM.896243 Text en © Med Sci Monit, 2015 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
spellingShingle Clinical Research
Zheng, Ji-shun
Chen, Meng-meng
Yang, Hai-fei
Zhou, Xiang-tian
Liu, Yan-yan
Li, Jia-bin
Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B
title Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B
title_full Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B
title_fullStr Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B
title_full_unstemmed Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B
title_short Stool vs. Serum Hepatitis B Virus DNA in Patients with Chronic Hepatitis B
title_sort stool vs. serum hepatitis b virus dna in patients with chronic hepatitis b
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4677737/
https://www.ncbi.nlm.nih.gov/pubmed/26645150
http://dx.doi.org/10.12659/MSM.896243
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