Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants

We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplici...

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Detalles Bibliográficos
Autores principales: Lee, Siwon, Kim, Jin-Ho, Choi, Ji-Young, Jang, Won-Cheoul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2015
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4677754/
https://www.ncbi.nlm.nih.gov/pubmed/26674930
http://dx.doi.org/10.5423/PPJ.NT.06.2015.0110
Descripción
Sumario:We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.

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