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CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface

Vesicle-associated membrane protein–associated protein (VAP) is an endoplasmic reticulum (ER)-resident integral membrane protein that controls a nonvesicular mode of ceramide and cholesterol transfer from the ER to the Golgi complex by interacting with ceramide transfer protein and oxysterol-binding...

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Autores principales: Wakana, Yuichi, Kotake, Richika, Oyama, Nanako, Murate, Motohide, Kobayashi, Toshihide, Arasaki, Kohei, Inoue, Hiroki, Tagaya, Mitsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678024/
https://www.ncbi.nlm.nih.gov/pubmed/26490117
http://dx.doi.org/10.1091/mbc.E15-08-0599
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author Wakana, Yuichi
Kotake, Richika
Oyama, Nanako
Murate, Motohide
Kobayashi, Toshihide
Arasaki, Kohei
Inoue, Hiroki
Tagaya, Mitsuo
author_facet Wakana, Yuichi
Kotake, Richika
Oyama, Nanako
Murate, Motohide
Kobayashi, Toshihide
Arasaki, Kohei
Inoue, Hiroki
Tagaya, Mitsuo
author_sort Wakana, Yuichi
collection PubMed
description Vesicle-associated membrane protein–associated protein (VAP) is an endoplasmic reticulum (ER)-resident integral membrane protein that controls a nonvesicular mode of ceramide and cholesterol transfer from the ER to the Golgi complex by interacting with ceramide transfer protein and oxysterol-binding protein (OSBP), respectively. We report that VAP and its interacting proteins are required for the processing and secretion of pancreatic adenocarcinoma up-regulated factor, whose transport from the trans-Golgi network (TGN) to the cell surface is mediated by transport carriers called “carriers of the trans-Golgi network to the cell surface” (CARTS). In VAP-depleted cells, diacylglycerol level at the TGN was decreased and CARTS formation was impaired. We found that VAP forms a complex with not only OSBP but also Sac1 phosphoinositide phosphatase at specialized ER subdomains that are closely apposed to the trans-Golgi/TGN, most likely reflecting membrane contact sites. Immobilization of ER–Golgi contacts dramatically reduced CARTS production, indicating that association–dissociation dynamics of the two membranes are important. On the basis of these findings, we propose that the ER–Golgi contacts play a pivotal role in lipid metabolism to control the biogenesis of transport carriers from the TGN.
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spelling pubmed-46780242016-03-01 CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface Wakana, Yuichi Kotake, Richika Oyama, Nanako Murate, Motohide Kobayashi, Toshihide Arasaki, Kohei Inoue, Hiroki Tagaya, Mitsuo Mol Biol Cell Articles Vesicle-associated membrane protein–associated protein (VAP) is an endoplasmic reticulum (ER)-resident integral membrane protein that controls a nonvesicular mode of ceramide and cholesterol transfer from the ER to the Golgi complex by interacting with ceramide transfer protein and oxysterol-binding protein (OSBP), respectively. We report that VAP and its interacting proteins are required for the processing and secretion of pancreatic adenocarcinoma up-regulated factor, whose transport from the trans-Golgi network (TGN) to the cell surface is mediated by transport carriers called “carriers of the trans-Golgi network to the cell surface” (CARTS). In VAP-depleted cells, diacylglycerol level at the TGN was decreased and CARTS formation was impaired. We found that VAP forms a complex with not only OSBP but also Sac1 phosphoinositide phosphatase at specialized ER subdomains that are closely apposed to the trans-Golgi/TGN, most likely reflecting membrane contact sites. Immobilization of ER–Golgi contacts dramatically reduced CARTS production, indicating that association–dissociation dynamics of the two membranes are important. On the basis of these findings, we propose that the ER–Golgi contacts play a pivotal role in lipid metabolism to control the biogenesis of transport carriers from the TGN. The American Society for Cell Biology 2015-12-15 /pmc/articles/PMC4678024/ /pubmed/26490117 http://dx.doi.org/10.1091/mbc.E15-08-0599 Text en © 2015 Wakana et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Wakana, Yuichi
Kotake, Richika
Oyama, Nanako
Murate, Motohide
Kobayashi, Toshihide
Arasaki, Kohei
Inoue, Hiroki
Tagaya, Mitsuo
CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface
title CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface
title_full CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface
title_fullStr CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface
title_full_unstemmed CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface
title_short CARTS biogenesis requires VAP–lipid transfer protein complexes functioning at the endoplasmic reticulum–Golgi interface
title_sort carts biogenesis requires vap–lipid transfer protein complexes functioning at the endoplasmic reticulum–golgi interface
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678024/
https://www.ncbi.nlm.nih.gov/pubmed/26490117
http://dx.doi.org/10.1091/mbc.E15-08-0599
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