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Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis

Acute colitis causes alterations in the intestinal microbiota, but the microbiota is thought to recover after such events. Extreme microbiota alterations are characteristic of human chronic inflammatory bowel diseases, although alterations reported in different studies are divergent and sometimes ev...

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Autores principales: Berry, David, Kuzyk, Orest, Rauch, Isabella, Heider, Susanne, Schwab, Clarissa, Hainzl, Eva, Decker, Thomas, Müller, Mathias, Strobl, Birgit, Schleper, Christa, Urich, Tim, Wagner, Michael, Kenner, Lukas, Loy, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678223/
https://www.ncbi.nlm.nih.gov/pubmed/26697002
http://dx.doi.org/10.3389/fmicb.2015.01408
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author Berry, David
Kuzyk, Orest
Rauch, Isabella
Heider, Susanne
Schwab, Clarissa
Hainzl, Eva
Decker, Thomas
Müller, Mathias
Strobl, Birgit
Schleper, Christa
Urich, Tim
Wagner, Michael
Kenner, Lukas
Loy, Alexander
author_facet Berry, David
Kuzyk, Orest
Rauch, Isabella
Heider, Susanne
Schwab, Clarissa
Hainzl, Eva
Decker, Thomas
Müller, Mathias
Strobl, Birgit
Schleper, Christa
Urich, Tim
Wagner, Michael
Kenner, Lukas
Loy, Alexander
author_sort Berry, David
collection PubMed
description Acute colitis causes alterations in the intestinal microbiota, but the microbiota is thought to recover after such events. Extreme microbiota alterations are characteristic of human chronic inflammatory bowel diseases, although alterations reported in different studies are divergent and sometimes even contradictory. To better understand the impact of periodic disturbances on the intestinal microbiota and its compositional difference between acute and relapsing colitis, we investigated the beginnings of recurrent inflammation using the dextran sodium sulfate (DSS) mouse model of chemically induced colitis. Using bacterial 16S rRNA gene-targeted pyrosequencing as well as quantitative fluorescence in situ hybridization, we profiled the intestinal and stool microbiota of mice over the course of three rounds of DSS-induced colitis and recovery. We found that characteristic inflammation-associated microbiota could be detected in recovery-phase mice. Successive inflammation episodes further drove the microbiota into an increasingly altered composition post-inflammation, and signatures of colitis history were detectable in the microbiota more sensitively than by pathology analysis. Bacterial indicators of murine colitis history were identified in intestinal and stool samples, with a high degree of consistency between both sample types. Stool may therefore be a promising non-invasive source of bacterial biomarkers that are highly sensitive to inflammation state and history.
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spelling pubmed-46782232015-12-22 Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis Berry, David Kuzyk, Orest Rauch, Isabella Heider, Susanne Schwab, Clarissa Hainzl, Eva Decker, Thomas Müller, Mathias Strobl, Birgit Schleper, Christa Urich, Tim Wagner, Michael Kenner, Lukas Loy, Alexander Front Microbiol Microbiology Acute colitis causes alterations in the intestinal microbiota, but the microbiota is thought to recover after such events. Extreme microbiota alterations are characteristic of human chronic inflammatory bowel diseases, although alterations reported in different studies are divergent and sometimes even contradictory. To better understand the impact of periodic disturbances on the intestinal microbiota and its compositional difference between acute and relapsing colitis, we investigated the beginnings of recurrent inflammation using the dextran sodium sulfate (DSS) mouse model of chemically induced colitis. Using bacterial 16S rRNA gene-targeted pyrosequencing as well as quantitative fluorescence in situ hybridization, we profiled the intestinal and stool microbiota of mice over the course of three rounds of DSS-induced colitis and recovery. We found that characteristic inflammation-associated microbiota could be detected in recovery-phase mice. Successive inflammation episodes further drove the microbiota into an increasingly altered composition post-inflammation, and signatures of colitis history were detectable in the microbiota more sensitively than by pathology analysis. Bacterial indicators of murine colitis history were identified in intestinal and stool samples, with a high degree of consistency between both sample types. Stool may therefore be a promising non-invasive source of bacterial biomarkers that are highly sensitive to inflammation state and history. Frontiers Media S.A. 2015-12-15 /pmc/articles/PMC4678223/ /pubmed/26697002 http://dx.doi.org/10.3389/fmicb.2015.01408 Text en Copyright © 2015 Berry, Kuzyk, Rauch, Heider, Schwab, Hainzl, Decker, Müller, Strobl, Schleper, Urich, Wagner, Kenner and Loy. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Berry, David
Kuzyk, Orest
Rauch, Isabella
Heider, Susanne
Schwab, Clarissa
Hainzl, Eva
Decker, Thomas
Müller, Mathias
Strobl, Birgit
Schleper, Christa
Urich, Tim
Wagner, Michael
Kenner, Lukas
Loy, Alexander
Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis
title Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis
title_full Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis
title_fullStr Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis
title_full_unstemmed Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis
title_short Intestinal Microbiota Signatures Associated with Inflammation History in Mice Experiencing Recurring Colitis
title_sort intestinal microbiota signatures associated with inflammation history in mice experiencing recurring colitis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678223/
https://www.ncbi.nlm.nih.gov/pubmed/26697002
http://dx.doi.org/10.3389/fmicb.2015.01408
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