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Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy
BACKGROUND: Plasmodium falciparum malaria is a significant public health problem in Comoros, and artemisinin combination therapy (ACT) remains the first choice for treating acute uncomplicated P. falciparum. The emergence and spread of artemisinin-resistant P. falciparum in Southeast Asia, associate...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678476/ https://www.ncbi.nlm.nih.gov/pubmed/26667053 http://dx.doi.org/10.1186/s13071-015-1253-z |
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author | Huang, Bo Deng, Changsheng Yang, Tao Xue, Linlu Wang, Qi Huang, Shiguang Su, Xin-zhuan Liu, Yajun Zheng, Shaoqin Guan, Yezhi Xu, Qin Zhou, Jiuyao Yuan, Jie Bacar, Afane Abdallah, Kamal Said Attoumane, Rachad Mliva, Ahamada M. S. A. Zhong, Yanchun Lu, Fangli Song, Jianping |
author_facet | Huang, Bo Deng, Changsheng Yang, Tao Xue, Linlu Wang, Qi Huang, Shiguang Su, Xin-zhuan Liu, Yajun Zheng, Shaoqin Guan, Yezhi Xu, Qin Zhou, Jiuyao Yuan, Jie Bacar, Afane Abdallah, Kamal Said Attoumane, Rachad Mliva, Ahamada M. S. A. Zhong, Yanchun Lu, Fangli Song, Jianping |
author_sort | Huang, Bo |
collection | PubMed |
description | BACKGROUND: Plasmodium falciparum malaria is a significant public health problem in Comoros, and artemisinin combination therapy (ACT) remains the first choice for treating acute uncomplicated P. falciparum. The emergence and spread of artemisinin-resistant P. falciparum in Southeast Asia, associated with mutations in K13-propeller gene, poses a potential threat to ACT efficacy. Detection of mutations in the P. falciparum K13-propeller gene may provide the first-hand information on changes in parasite susceptibility to artemisinin. The objective of this study is to determinate the prevalence of mutant K13-propeller gene among the P. falciparum isolates collected from Grande Comore Island, Union of Comoros, where ACT has been in use since 2004. METHODS: A total of 207 P. falciparum clinical isolates were collected from the island during March 2006 and October 2007 (n = 118) and March 2013 and December 2014 (n = 89). All isolates were analysed for single nucleotide polymorphisms (SNPs) and haplotypes in the K13-propeller gene using nested PCR and DNA sequencing. RESULTS: Only three 2006–2007 samples carried SNPs in the K13-propeller gene, one having a synonymous (G538G) and the other having two non-synonymous (S477Y and D584E) substitutions leading to two mutated haplotypes (2.2 %, 2/95). Three synonymous mutations (R471R, Y500Y, and G538G) (5.9 %, 5/85) and 7 non-synonymous substitutions (21.2 %, 18/85) with nine mutated haplotypes (18.8 %, 16/85) were found in isolates from 2013 to 2014. However, none of the polymorphisms associated with artemisinin-resistance in Southeast Asia was detected from any of the parasites examined. CONCLUSION: This study showed increased K13-propeller gene diversity among P. falciparum populations on the Island over the course of 8 years (2006–2014). Nevertheless, none of the polymorphisms known to be associated with artemisinin resistance in Asia was detected in the parasite populations examined. Our data suggest that P. falciparum populations in Grande Comore are still effectively susceptible to artemisinin. Our results provide insights into P. falciparum populations regarding mutations in the gene associated with artemisinin resistance and will be useful for developing and updating anti-malarial guidance in Comoros. |
format | Online Article Text |
id | pubmed-4678476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46784762015-12-16 Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy Huang, Bo Deng, Changsheng Yang, Tao Xue, Linlu Wang, Qi Huang, Shiguang Su, Xin-zhuan Liu, Yajun Zheng, Shaoqin Guan, Yezhi Xu, Qin Zhou, Jiuyao Yuan, Jie Bacar, Afane Abdallah, Kamal Said Attoumane, Rachad Mliva, Ahamada M. S. A. Zhong, Yanchun Lu, Fangli Song, Jianping Parasit Vectors Research BACKGROUND: Plasmodium falciparum malaria is a significant public health problem in Comoros, and artemisinin combination therapy (ACT) remains the first choice for treating acute uncomplicated P. falciparum. The emergence and spread of artemisinin-resistant P. falciparum in Southeast Asia, associated with mutations in K13-propeller gene, poses a potential threat to ACT efficacy. Detection of mutations in the P. falciparum K13-propeller gene may provide the first-hand information on changes in parasite susceptibility to artemisinin. The objective of this study is to determinate the prevalence of mutant K13-propeller gene among the P. falciparum isolates collected from Grande Comore Island, Union of Comoros, where ACT has been in use since 2004. METHODS: A total of 207 P. falciparum clinical isolates were collected from the island during March 2006 and October 2007 (n = 118) and March 2013 and December 2014 (n = 89). All isolates were analysed for single nucleotide polymorphisms (SNPs) and haplotypes in the K13-propeller gene using nested PCR and DNA sequencing. RESULTS: Only three 2006–2007 samples carried SNPs in the K13-propeller gene, one having a synonymous (G538G) and the other having two non-synonymous (S477Y and D584E) substitutions leading to two mutated haplotypes (2.2 %, 2/95). Three synonymous mutations (R471R, Y500Y, and G538G) (5.9 %, 5/85) and 7 non-synonymous substitutions (21.2 %, 18/85) with nine mutated haplotypes (18.8 %, 16/85) were found in isolates from 2013 to 2014. However, none of the polymorphisms associated with artemisinin-resistance in Southeast Asia was detected from any of the parasites examined. CONCLUSION: This study showed increased K13-propeller gene diversity among P. falciparum populations on the Island over the course of 8 years (2006–2014). Nevertheless, none of the polymorphisms known to be associated with artemisinin resistance in Asia was detected in the parasite populations examined. Our data suggest that P. falciparum populations in Grande Comore are still effectively susceptible to artemisinin. Our results provide insights into P. falciparum populations regarding mutations in the gene associated with artemisinin resistance and will be useful for developing and updating anti-malarial guidance in Comoros. BioMed Central 2015-12-15 /pmc/articles/PMC4678476/ /pubmed/26667053 http://dx.doi.org/10.1186/s13071-015-1253-z Text en © Huang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Huang, Bo Deng, Changsheng Yang, Tao Xue, Linlu Wang, Qi Huang, Shiguang Su, Xin-zhuan Liu, Yajun Zheng, Shaoqin Guan, Yezhi Xu, Qin Zhou, Jiuyao Yuan, Jie Bacar, Afane Abdallah, Kamal Said Attoumane, Rachad Mliva, Ahamada M. S. A. Zhong, Yanchun Lu, Fangli Song, Jianping Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy |
title | Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy |
title_full | Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy |
title_fullStr | Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy |
title_full_unstemmed | Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy |
title_short | Polymorphisms of the artemisinin resistant marker (K13) in Plasmodium falciparum parasite populations of Grande Comore Island 10 years after artemisinin combination therapy |
title_sort | polymorphisms of the artemisinin resistant marker (k13) in plasmodium falciparum parasite populations of grande comore island 10 years after artemisinin combination therapy |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678476/ https://www.ncbi.nlm.nih.gov/pubmed/26667053 http://dx.doi.org/10.1186/s13071-015-1253-z |
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