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Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction

BACKGROUND: In the present scenario, we are made available with chemomechanical caries removal system containing a natural proteolytic enzyme for the ease in the excavation of infected dentine. The additive action for these agents is providing antimicrobial and anti-inflammatory properties. AIM: Thi...

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Autores principales: Kush, Anil, Thakur, Rachna, Patil, Sandya Devi S., Paul, Santhosh T., Kakanur, Madhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678554/
https://www.ncbi.nlm.nih.gov/pubmed/26681861
http://dx.doi.org/10.4103/0976-237X.169860
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author Kush, Anil
Thakur, Rachna
Patil, Sandya Devi S.
Paul, Santhosh T.
Kakanur, Madhu
author_facet Kush, Anil
Thakur, Rachna
Patil, Sandya Devi S.
Paul, Santhosh T.
Kakanur, Madhu
author_sort Kush, Anil
collection PubMed
description BACKGROUND: In the present scenario, we are made available with chemomechanical caries removal system containing a natural proteolytic enzyme for the ease in the excavation of infected dentine. The additive action for these agents is providing antimicrobial and anti-inflammatory properties. AIM: This study was undertaken for assessing the action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans. MATERIALS AND METHODS: The samples were collected for cultivation of the periodontal pathogen from the clinical periodontal pockets using sterile paper points. The samples cultured under suitable conditions were analyzed with quantitative polymerase chain reaction targeting 16s r-DNA. The samples were divided into three groups namely, Group A: Control, Group B: With Papacarie Duo, Group C: With Carie Care. The pathogen inoculums plugs were inserted in the petri dishes containing chemically defined medium and the experimental gels at different concentrations and were incubated under optimal conditions. The inhibition of growth of the pathogen was studied visually. RESULTS: There was visual inhibition of growth for Group B and C and also exhibited a dose-dependent effect also. CONCLUSION: Based on the results of the present study, Carie Care™ gel demonstrated better antimicrobial action against A. actinomycetemcomitans which is a major periodontal disease causing pathogen.
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spelling pubmed-46785542015-12-17 Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction Kush, Anil Thakur, Rachna Patil, Sandya Devi S. Paul, Santhosh T. Kakanur, Madhu Contemp Clin Dent Original Article BACKGROUND: In the present scenario, we are made available with chemomechanical caries removal system containing a natural proteolytic enzyme for the ease in the excavation of infected dentine. The additive action for these agents is providing antimicrobial and anti-inflammatory properties. AIM: This study was undertaken for assessing the action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans. MATERIALS AND METHODS: The samples were collected for cultivation of the periodontal pathogen from the clinical periodontal pockets using sterile paper points. The samples cultured under suitable conditions were analyzed with quantitative polymerase chain reaction targeting 16s r-DNA. The samples were divided into three groups namely, Group A: Control, Group B: With Papacarie Duo, Group C: With Carie Care. The pathogen inoculums plugs were inserted in the petri dishes containing chemically defined medium and the experimental gels at different concentrations and were incubated under optimal conditions. The inhibition of growth of the pathogen was studied visually. RESULTS: There was visual inhibition of growth for Group B and C and also exhibited a dose-dependent effect also. CONCLUSION: Based on the results of the present study, Carie Care™ gel demonstrated better antimicrobial action against A. actinomycetemcomitans which is a major periodontal disease causing pathogen. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4678554/ /pubmed/26681861 http://dx.doi.org/10.4103/0976-237X.169860 Text en Copyright: © Contemporary Clinical Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kush, Anil
Thakur, Rachna
Patil, Sandya Devi S.
Paul, Santhosh T.
Kakanur, Madhu
Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction
title Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction
title_full Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction
title_fullStr Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction
title_full_unstemmed Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction
title_short Evaluation of antimicrobial action of Carie Care™ and Papacarie Duo™ on Aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction
title_sort evaluation of antimicrobial action of carie care™ and papacarie duo™ on aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678554/
https://www.ncbi.nlm.nih.gov/pubmed/26681861
http://dx.doi.org/10.4103/0976-237X.169860
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