No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach

BACKGROUND: The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present i...

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Autores principales: Berthet, Nicolas, Frangeul, Lionel, Olaussen, Ken André, Brambilla, Elisabeth, Dorvault, Nicolas, Girard, Philippe, Validire, Pierre, Fadel, Elie, Bouchier, Christiane, Gessain, Antoine, Soria, Jean-Charles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678645/
https://www.ncbi.nlm.nih.gov/pubmed/26667652
http://dx.doi.org/10.1186/s13104-015-1669-8
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author Berthet, Nicolas
Frangeul, Lionel
Olaussen, Ken André
Brambilla, Elisabeth
Dorvault, Nicolas
Girard, Philippe
Validire, Pierre
Fadel, Elie
Bouchier, Christiane
Gessain, Antoine
Soria, Jean-Charles
author_facet Berthet, Nicolas
Frangeul, Lionel
Olaussen, Ken André
Brambilla, Elisabeth
Dorvault, Nicolas
Girard, Philippe
Validire, Pierre
Fadel, Elie
Bouchier, Christiane
Gessain, Antoine
Soria, Jean-Charles
author_sort Berthet, Nicolas
collection PubMed
description BACKGROUND: The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample. RESULTS: None of the reads showed a significant similarity for Jaagsiekte sheep retrovirus (JSRV) and no other viruses were found except for endogenous retroviruses. CONCLUSIONS: In conclusion, we have demonstrated the absence of JSRV and other known human viruses in five samples of well-characterized lepidic adenocarcinoma. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-015-1669-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-46786452015-12-16 No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach Berthet, Nicolas Frangeul, Lionel Olaussen, Ken André Brambilla, Elisabeth Dorvault, Nicolas Girard, Philippe Validire, Pierre Fadel, Elie Bouchier, Christiane Gessain, Antoine Soria, Jean-Charles BMC Res Notes Research Article BACKGROUND: The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample. RESULTS: None of the reads showed a significant similarity for Jaagsiekte sheep retrovirus (JSRV) and no other viruses were found except for endogenous retroviruses. CONCLUSIONS: In conclusion, we have demonstrated the absence of JSRV and other known human viruses in five samples of well-characterized lepidic adenocarcinoma. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-015-1669-8) contains supplementary material, which is available to authorized users. BioMed Central 2015-12-15 /pmc/articles/PMC4678645/ /pubmed/26667652 http://dx.doi.org/10.1186/s13104-015-1669-8 Text en © Berthet et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Berthet, Nicolas
Frangeul, Lionel
Olaussen, Ken André
Brambilla, Elisabeth
Dorvault, Nicolas
Girard, Philippe
Validire, Pierre
Fadel, Elie
Bouchier, Christiane
Gessain, Antoine
Soria, Jean-Charles
No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach
title No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach
title_full No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach
title_fullStr No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach
title_full_unstemmed No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach
title_short No evidence for viral sequences in five lepidic adenocarcinomas (former “BAC”) by a high-throughput sequencing approach
title_sort no evidence for viral sequences in five lepidic adenocarcinomas (former “bac”) by a high-throughput sequencing approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678645/
https://www.ncbi.nlm.nih.gov/pubmed/26667652
http://dx.doi.org/10.1186/s13104-015-1669-8
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