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PacBio Sequencing and Its Applications
Single-molecule, real-time sequencing developed by Pacific BioSciences offers longer read lengths than the second-generation sequencing (SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research. The highly-contiguous de novo assemblies using P...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678779/ https://www.ncbi.nlm.nih.gov/pubmed/26542840 http://dx.doi.org/10.1016/j.gpb.2015.08.002 |
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author | Rhoads, Anthony Au, Kin Fai |
author_facet | Rhoads, Anthony Au, Kin Fai |
author_sort | Rhoads, Anthony |
collection | PubMed |
description | Single-molecule, real-time sequencing developed by Pacific BioSciences offers longer read lengths than the second-generation sequencing (SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research. The highly-contiguous de novo assemblies using PacBio sequencing can close gaps in current reference assemblies and characterize structural variation (SV) in personal genomes. With longer reads, we can sequence through extended repetitive regions and detect mutations, many of which are associated with diseases. Moreover, PacBio transcriptome sequencing is advantageous for the identification of gene isoforms and facilitates reliable discoveries of novel genes and novel isoforms of annotated genes, due to its ability to sequence full-length transcripts or fragments with significant lengths. Additionally, PacBio’s sequencing technique provides information that is useful for the direct detection of base modifications, such as methylation. In addition to using PacBio sequencing alone, many hybrid sequencing strategies have been developed to make use of more accurate short reads in conjunction with PacBio long reads. In general, hybrid sequencing strategies are more affordable and scalable especially for small-size laboratories than using PacBio Sequencing alone. The advent of PacBio sequencing has made available much information that could not be obtained via SGS alone. |
format | Online Article Text |
id | pubmed-4678779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-46787792016-01-06 PacBio Sequencing and Its Applications Rhoads, Anthony Au, Kin Fai Genomics Proteomics Bioinformatics Review Single-molecule, real-time sequencing developed by Pacific BioSciences offers longer read lengths than the second-generation sequencing (SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research. The highly-contiguous de novo assemblies using PacBio sequencing can close gaps in current reference assemblies and characterize structural variation (SV) in personal genomes. With longer reads, we can sequence through extended repetitive regions and detect mutations, many of which are associated with diseases. Moreover, PacBio transcriptome sequencing is advantageous for the identification of gene isoforms and facilitates reliable discoveries of novel genes and novel isoforms of annotated genes, due to its ability to sequence full-length transcripts or fragments with significant lengths. Additionally, PacBio’s sequencing technique provides information that is useful for the direct detection of base modifications, such as methylation. In addition to using PacBio sequencing alone, many hybrid sequencing strategies have been developed to make use of more accurate short reads in conjunction with PacBio long reads. In general, hybrid sequencing strategies are more affordable and scalable especially for small-size laboratories than using PacBio Sequencing alone. The advent of PacBio sequencing has made available much information that could not be obtained via SGS alone. Elsevier 2015-10 2015-11-02 /pmc/articles/PMC4678779/ /pubmed/26542840 http://dx.doi.org/10.1016/j.gpb.2015.08.002 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Rhoads, Anthony Au, Kin Fai PacBio Sequencing and Its Applications |
title | PacBio Sequencing and Its Applications |
title_full | PacBio Sequencing and Its Applications |
title_fullStr | PacBio Sequencing and Its Applications |
title_full_unstemmed | PacBio Sequencing and Its Applications |
title_short | PacBio Sequencing and Its Applications |
title_sort | pacbio sequencing and its applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678779/ https://www.ncbi.nlm.nih.gov/pubmed/26542840 http://dx.doi.org/10.1016/j.gpb.2015.08.002 |
work_keys_str_mv | AT rhoadsanthony pacbiosequencinganditsapplications AT aukinfai pacbiosequencinganditsapplications |