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A polymerase engineered for bisulfite sequencing

Bisulfite sequencing is a key methodology in epigenetics. However, the standard workflow of bisulfite sequencing involves heat and strongly basic conditions to convert the intermediary product 5,6-dihydrouridine-6-sulfonate (dhU6S) (generated by reaction of bisulfite with deoxycytidine (dC)) to urac...

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Detalles Bibliográficos
Autores principales:	Millar, Doug, Christova, Yonka, Holliger, Philipp
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678845/ https://www.ncbi.nlm.nih.gov/pubmed/26271989 http://dx.doi.org/10.1093/nar/gkv798

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