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Production of ascorbic acid releasing biomaterials for pelvic floor repair

OBJECTIVE: An underlying abnormality in collagen turnover is implied in the occurrence of complications and recurrences after mesh augmented pelvic floor repair surgeries. Ascorbic acid is a potent stimulant of collagen synthesis. The aim of this study is to produce ascorbic acid releasing poly-lact...

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Autores principales: Mangır, Naşide, Bullock, Anthony J., Roman, Sabiniano, Osman, Nadir, Chapple, Christopher, MacNeil, Sheila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678952/
https://www.ncbi.nlm.nih.gov/pubmed/26478470
http://dx.doi.org/10.1016/j.actbio.2015.10.019
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author Mangır, Naşide
Bullock, Anthony J.
Roman, Sabiniano
Osman, Nadir
Chapple, Christopher
MacNeil, Sheila
author_facet Mangır, Naşide
Bullock, Anthony J.
Roman, Sabiniano
Osman, Nadir
Chapple, Christopher
MacNeil, Sheila
author_sort Mangır, Naşide
collection PubMed
description OBJECTIVE: An underlying abnormality in collagen turnover is implied in the occurrence of complications and recurrences after mesh augmented pelvic floor repair surgeries. Ascorbic acid is a potent stimulant of collagen synthesis. The aim of this study is to produce ascorbic acid releasing poly-lactic acid (PLA) scaffolds and evaluate them for their effects on extracellular matrix production and the strength of the materials. MATERIALS AND METHODS: Scaffolds which contained either l-ascorbic acid (AA) and Ascorbate-2-Phosphate (A2P) were produced with emulsion electrospinning. The release of both drugs was measured by UV spectrophotometry. Human dermal fibroblasts were seeded on scaffolds and cultured for 2 weeks. Cell attachment, viability and total collagen production were evaluated as well as mechanical properties. RESULTS: No significant differences were observed between AA, A2P, Vehicle and PLA scaffolds in terms of fibre diameter and pore size. The encapsulation efficiency and successful release of both AA and A2P were demonstrated. Both AA and A2P containing scaffolds were significantly more hydrophilic and stronger in both dry and wet states compared to PLA scaffolds. Fibroblasts produced more collagen on scaffolds containing either AA or A2P compared to cells grown on control scaffolds. CONCLUSION: This study is the first to directly compare the two ascorbic acid derivatives in a tissue engineered scaffold and shows that both AA and A2P releasing electrospun PLA scaffolds increased collagen production of fibroblasts to similar extents but AA scaffolds seemed to be more hydrophilic and stronger compared to A2P scaffolds. STATEMENT OF SIGNIFICANCE: Mesh augmented surgical repair of the pelvic floor currently relies on non-degradable materials which results in severe complications in some patients. There is an unmet and urgent need for better pelvic floor repair materials. Our current understanding suggests that the ideal material should be able to better integrate into sites of implantation both biologically and mechanically. The impact of vitamin C on extracellular matrix production is well established but we in this study have undertaken a critical comparison of two derivatives of vitamin C as they are released from a biodegradable scaffold. This strategy proved to be equally useful with both derivatives in terms of new tissue production yet we observed significant differences in mechanical properties of these biomaterials.
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spelling pubmed-46789522016-01-04 Production of ascorbic acid releasing biomaterials for pelvic floor repair Mangır, Naşide Bullock, Anthony J. Roman, Sabiniano Osman, Nadir Chapple, Christopher MacNeil, Sheila Acta Biomater Article OBJECTIVE: An underlying abnormality in collagen turnover is implied in the occurrence of complications and recurrences after mesh augmented pelvic floor repair surgeries. Ascorbic acid is a potent stimulant of collagen synthesis. The aim of this study is to produce ascorbic acid releasing poly-lactic acid (PLA) scaffolds and evaluate them for their effects on extracellular matrix production and the strength of the materials. MATERIALS AND METHODS: Scaffolds which contained either l-ascorbic acid (AA) and Ascorbate-2-Phosphate (A2P) were produced with emulsion electrospinning. The release of both drugs was measured by UV spectrophotometry. Human dermal fibroblasts were seeded on scaffolds and cultured for 2 weeks. Cell attachment, viability and total collagen production were evaluated as well as mechanical properties. RESULTS: No significant differences were observed between AA, A2P, Vehicle and PLA scaffolds in terms of fibre diameter and pore size. The encapsulation efficiency and successful release of both AA and A2P were demonstrated. Both AA and A2P containing scaffolds were significantly more hydrophilic and stronger in both dry and wet states compared to PLA scaffolds. Fibroblasts produced more collagen on scaffolds containing either AA or A2P compared to cells grown on control scaffolds. CONCLUSION: This study is the first to directly compare the two ascorbic acid derivatives in a tissue engineered scaffold and shows that both AA and A2P releasing electrospun PLA scaffolds increased collagen production of fibroblasts to similar extents but AA scaffolds seemed to be more hydrophilic and stronger compared to A2P scaffolds. STATEMENT OF SIGNIFICANCE: Mesh augmented surgical repair of the pelvic floor currently relies on non-degradable materials which results in severe complications in some patients. There is an unmet and urgent need for better pelvic floor repair materials. Our current understanding suggests that the ideal material should be able to better integrate into sites of implantation both biologically and mechanically. The impact of vitamin C on extracellular matrix production is well established but we in this study have undertaken a critical comparison of two derivatives of vitamin C as they are released from a biodegradable scaffold. This strategy proved to be equally useful with both derivatives in terms of new tissue production yet we observed significant differences in mechanical properties of these biomaterials. Elsevier 2016-01-01 /pmc/articles/PMC4678952/ /pubmed/26478470 http://dx.doi.org/10.1016/j.actbio.2015.10.019 Text en © 2015 Elsevier Ltd. All rights reserved. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mangır, Naşide
Bullock, Anthony J.
Roman, Sabiniano
Osman, Nadir
Chapple, Christopher
MacNeil, Sheila
Production of ascorbic acid releasing biomaterials for pelvic floor repair
title Production of ascorbic acid releasing biomaterials for pelvic floor repair
title_full Production of ascorbic acid releasing biomaterials for pelvic floor repair
title_fullStr Production of ascorbic acid releasing biomaterials for pelvic floor repair
title_full_unstemmed Production of ascorbic acid releasing biomaterials for pelvic floor repair
title_short Production of ascorbic acid releasing biomaterials for pelvic floor repair
title_sort production of ascorbic acid releasing biomaterials for pelvic floor repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678952/
https://www.ncbi.nlm.nih.gov/pubmed/26478470
http://dx.doi.org/10.1016/j.actbio.2015.10.019
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