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A Wash-Free Homogeneous Colorimetric Immunoassay Method
Rapid and convenient biosensing platforms could be beneficial to timely diagnosis and treatment of diseases in virtually any care settings. Sandwich immunoassays, the most commonly used methods for protein detection, often rely on expensive tags such as enzyme and tedious wash and incubation procedu...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679354/ https://www.ncbi.nlm.nih.gov/pubmed/26722373 http://dx.doi.org/10.7150/thno.13159 |
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author | Liu, Huiqiao Rong, Pengfei Jia, Hongwei Yang, Jie Dong, Bo Dong, Qiong Yang, Cejun Hu, Pengzhi Wang, Wei Liu, Haitao Liu, Dingbin |
author_facet | Liu, Huiqiao Rong, Pengfei Jia, Hongwei Yang, Jie Dong, Bo Dong, Qiong Yang, Cejun Hu, Pengzhi Wang, Wei Liu, Haitao Liu, Dingbin |
author_sort | Liu, Huiqiao |
collection | PubMed |
description | Rapid and convenient biosensing platforms could be beneficial to timely diagnosis and treatment of diseases in virtually any care settings. Sandwich immunoassays, the most commonly used methods for protein detection, often rely on expensive tags such as enzyme and tedious wash and incubation procedures operated by skilled labor. In this report, we revolutionized traditional sandwich immunoassays by providing a wash-free homogeneous colorimetric immunoassay method without requirement of any separation steps. The proposed strategy was realized by controlling the growth of gold nanoparticles (AuNPs) to mediate the interparticle spacing in the protein-AuNP oligomers. We have demonstrated the successful in vitro detection of cancer biomarker in serum samples from patients with high clinical sensitivity and specificity. |
format | Online Article Text |
id | pubmed-4679354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-46793542016-01-01 A Wash-Free Homogeneous Colorimetric Immunoassay Method Liu, Huiqiao Rong, Pengfei Jia, Hongwei Yang, Jie Dong, Bo Dong, Qiong Yang, Cejun Hu, Pengzhi Wang, Wei Liu, Haitao Liu, Dingbin Theranostics Research Paper Rapid and convenient biosensing platforms could be beneficial to timely diagnosis and treatment of diseases in virtually any care settings. Sandwich immunoassays, the most commonly used methods for protein detection, often rely on expensive tags such as enzyme and tedious wash and incubation procedures operated by skilled labor. In this report, we revolutionized traditional sandwich immunoassays by providing a wash-free homogeneous colorimetric immunoassay method without requirement of any separation steps. The proposed strategy was realized by controlling the growth of gold nanoparticles (AuNPs) to mediate the interparticle spacing in the protein-AuNP oligomers. We have demonstrated the successful in vitro detection of cancer biomarker in serum samples from patients with high clinical sensitivity and specificity. Ivyspring International Publisher 2016-01-01 /pmc/articles/PMC4679354/ /pubmed/26722373 http://dx.doi.org/10.7150/thno.13159 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Liu, Huiqiao Rong, Pengfei Jia, Hongwei Yang, Jie Dong, Bo Dong, Qiong Yang, Cejun Hu, Pengzhi Wang, Wei Liu, Haitao Liu, Dingbin A Wash-Free Homogeneous Colorimetric Immunoassay Method |
title | A Wash-Free Homogeneous Colorimetric Immunoassay Method |
title_full | A Wash-Free Homogeneous Colorimetric Immunoassay Method |
title_fullStr | A Wash-Free Homogeneous Colorimetric Immunoassay Method |
title_full_unstemmed | A Wash-Free Homogeneous Colorimetric Immunoassay Method |
title_short | A Wash-Free Homogeneous Colorimetric Immunoassay Method |
title_sort | wash-free homogeneous colorimetric immunoassay method |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679354/ https://www.ncbi.nlm.nih.gov/pubmed/26722373 http://dx.doi.org/10.7150/thno.13159 |
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