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Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis

International efforts in developing a vaccine against Chlamydia trachomatis have highlighted the need for novel immunization strategies for the induction of genital immunity. In this study, we evaluated an intramuscular (IM) prime/intranasal boost vaccination strategy in a Göttingen Minipig model wi...

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Autores principales: Lorenzen, Emma, Follmann, Frank, Bøje, Sarah, Erneholm, Karin, Olsen, Anja Weinreich, Agerholm, Jørgen Steen, Jungersen, Gregers, Andersen, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679855/
https://www.ncbi.nlm.nih.gov/pubmed/26734002
http://dx.doi.org/10.3389/fimmu.2015.00628
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author Lorenzen, Emma
Follmann, Frank
Bøje, Sarah
Erneholm, Karin
Olsen, Anja Weinreich
Agerholm, Jørgen Steen
Jungersen, Gregers
Andersen, Peter
author_facet Lorenzen, Emma
Follmann, Frank
Bøje, Sarah
Erneholm, Karin
Olsen, Anja Weinreich
Agerholm, Jørgen Steen
Jungersen, Gregers
Andersen, Peter
author_sort Lorenzen, Emma
collection PubMed
description International efforts in developing a vaccine against Chlamydia trachomatis have highlighted the need for novel immunization strategies for the induction of genital immunity. In this study, we evaluated an intramuscular (IM) prime/intranasal boost vaccination strategy in a Göttingen Minipig model with a reproductive system very similar to humans. The vaccine was composed of C. trachomatis subunit antigens formulated in the Th1/Th17 promoting CAF01 adjuvant. IM priming immunizations with CAF01 induced a significant cell-mediated interferon gamma and interleukin 17A response and a significant systemic high-titered neutralizing IgG response. Following genital challenge, intranasally boosted groups mounted an accelerated, highly significant genital IgA response that correlated with enhanced bacterial clearance on day 3 post infection. By detecting antigen-specific secretory component (SC), we showed that the genital IgA was locally produced in the genital mucosa. The highly significant inverse correlation between the vaginal IgA SC response and the chlamydial load suggests that IgA in the minipig model is involved in protection against C. trachomatis. This is important both for our understanding of protective immunity and future vaccination strategies against C. trachomatis and genital pathogens in general.
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spelling pubmed-46798552016-01-05 Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis Lorenzen, Emma Follmann, Frank Bøje, Sarah Erneholm, Karin Olsen, Anja Weinreich Agerholm, Jørgen Steen Jungersen, Gregers Andersen, Peter Front Immunol Immunology International efforts in developing a vaccine against Chlamydia trachomatis have highlighted the need for novel immunization strategies for the induction of genital immunity. In this study, we evaluated an intramuscular (IM) prime/intranasal boost vaccination strategy in a Göttingen Minipig model with a reproductive system very similar to humans. The vaccine was composed of C. trachomatis subunit antigens formulated in the Th1/Th17 promoting CAF01 adjuvant. IM priming immunizations with CAF01 induced a significant cell-mediated interferon gamma and interleukin 17A response and a significant systemic high-titered neutralizing IgG response. Following genital challenge, intranasally boosted groups mounted an accelerated, highly significant genital IgA response that correlated with enhanced bacterial clearance on day 3 post infection. By detecting antigen-specific secretory component (SC), we showed that the genital IgA was locally produced in the genital mucosa. The highly significant inverse correlation between the vaginal IgA SC response and the chlamydial load suggests that IgA in the minipig model is involved in protection against C. trachomatis. This is important both for our understanding of protective immunity and future vaccination strategies against C. trachomatis and genital pathogens in general. Frontiers Media S.A. 2015-12-16 /pmc/articles/PMC4679855/ /pubmed/26734002 http://dx.doi.org/10.3389/fimmu.2015.00628 Text en Copyright © 2015 Lorenzen, Follmann, Bøje, Erneholm, Olsen, Agerholm, Jungersen and Andersen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lorenzen, Emma
Follmann, Frank
Bøje, Sarah
Erneholm, Karin
Olsen, Anja Weinreich
Agerholm, Jørgen Steen
Jungersen, Gregers
Andersen, Peter
Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis
title Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis
title_full Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis
title_fullStr Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis
title_full_unstemmed Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis
title_short Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity Through Secretory IgA in Minipigs Infected with Chlamydia trachomatis
title_sort intramuscular priming and intranasal boosting induce strong genital immunity through secretory iga in minipigs infected with chlamydia trachomatis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679855/
https://www.ncbi.nlm.nih.gov/pubmed/26734002
http://dx.doi.org/10.3389/fimmu.2015.00628
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