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Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China

Bioelectrochemical systems (BESs) are promising technologies for energy and product recovery coupled with wastewater treatment, and the core microbial community in electrochemically active biofilm in BESs remains controversy. In the present study, 7 anodic communities from 6 bioelectrochemical syste...

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Autores principales: Xiao, Yong, Zheng, Yue, Wu, Song, Zhang, En-Hua, Chen, Zheng, Liang, Peng, Huang, Xia, Yang, Zhao-Hui, Ng, I-Son, Chen, Bor-Yann, Zhao, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679932/
https://www.ncbi.nlm.nih.gov/pubmed/26733958
http://dx.doi.org/10.3389/fmicb.2015.01410
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author Xiao, Yong
Zheng, Yue
Wu, Song
Zhang, En-Hua
Chen, Zheng
Liang, Peng
Huang, Xia
Yang, Zhao-Hui
Ng, I-Son
Chen, Bor-Yann
Zhao, Feng
author_facet Xiao, Yong
Zheng, Yue
Wu, Song
Zhang, En-Hua
Chen, Zheng
Liang, Peng
Huang, Xia
Yang, Zhao-Hui
Ng, I-Son
Chen, Bor-Yann
Zhao, Feng
author_sort Xiao, Yong
collection PubMed
description Bioelectrochemical systems (BESs) are promising technologies for energy and product recovery coupled with wastewater treatment, and the core microbial community in electrochemically active biofilm in BESs remains controversy. In the present study, 7 anodic communities from 6 bioelectrochemical systems in 4 labs in southeast, north and south-central of China are explored by 454 pyrosequencing. A total of 251,225 effective sequences are obtained for 7 electrochemically active biofilm samples at 3% cutoff level. While Alpha-, Beta-, and Gamma-proteobacteria are the most abundant classes (averaging 16.0–17.7%), Bacteroidia and Clostridia are the two sub-dominant and commonly shared classes. Six commonly shared genera i.e., Azospira, Azospirillum, Acinetobacter, Bacteroides, Geobacter, Pseudomonas, and Rhodopseudomonas dominate the electrochemically active communities and are defined as core genera. A total of 25 OTUs with average relative abundance >0.5% were selected and designated as core OTUs, and some species relating to these OTUs have been reported electrochemically active. Furthermore, cyclic voltammetry and chronoamperometry tests show that two strains from Acinetobacter guillouiae and Stappia indica, bacteria relate to two core OTUs, are electrochemically active. Using randomly selected bioelectrochemical systems, the study has presented extremely diverse bacterial communities in anodic biofilms, though, we still can suggest some potentially microbes for investigating the electrochemical mechanisms in bioelectrochemical systems.
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spelling pubmed-46799322016-01-05 Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China Xiao, Yong Zheng, Yue Wu, Song Zhang, En-Hua Chen, Zheng Liang, Peng Huang, Xia Yang, Zhao-Hui Ng, I-Son Chen, Bor-Yann Zhao, Feng Front Microbiol Microbiology Bioelectrochemical systems (BESs) are promising technologies for energy and product recovery coupled with wastewater treatment, and the core microbial community in electrochemically active biofilm in BESs remains controversy. In the present study, 7 anodic communities from 6 bioelectrochemical systems in 4 labs in southeast, north and south-central of China are explored by 454 pyrosequencing. A total of 251,225 effective sequences are obtained for 7 electrochemically active biofilm samples at 3% cutoff level. While Alpha-, Beta-, and Gamma-proteobacteria are the most abundant classes (averaging 16.0–17.7%), Bacteroidia and Clostridia are the two sub-dominant and commonly shared classes. Six commonly shared genera i.e., Azospira, Azospirillum, Acinetobacter, Bacteroides, Geobacter, Pseudomonas, and Rhodopseudomonas dominate the electrochemically active communities and are defined as core genera. A total of 25 OTUs with average relative abundance >0.5% were selected and designated as core OTUs, and some species relating to these OTUs have been reported electrochemically active. Furthermore, cyclic voltammetry and chronoamperometry tests show that two strains from Acinetobacter guillouiae and Stappia indica, bacteria relate to two core OTUs, are electrochemically active. Using randomly selected bioelectrochemical systems, the study has presented extremely diverse bacterial communities in anodic biofilms, though, we still can suggest some potentially microbes for investigating the electrochemical mechanisms in bioelectrochemical systems. Frontiers Media S.A. 2015-12-16 /pmc/articles/PMC4679932/ /pubmed/26733958 http://dx.doi.org/10.3389/fmicb.2015.01410 Text en Copyright © 2015 Xiao, Zheng, Wu, Zhang, Chen, Liang, Huang, Yang, Ng, Chen and Zhao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xiao, Yong
Zheng, Yue
Wu, Song
Zhang, En-Hua
Chen, Zheng
Liang, Peng
Huang, Xia
Yang, Zhao-Hui
Ng, I-Son
Chen, Bor-Yann
Zhao, Feng
Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China
title Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China
title_full Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China
title_fullStr Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China
title_full_unstemmed Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China
title_short Pyrosequencing Reveals a Core Community of Anodic Bacterial Biofilms in Bioelectrochemical Systems from China
title_sort pyrosequencing reveals a core community of anodic bacterial biofilms in bioelectrochemical systems from china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679932/
https://www.ncbi.nlm.nih.gov/pubmed/26733958
http://dx.doi.org/10.3389/fmicb.2015.01410
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