There is no ‘Conundrum’ of InsP(6)

Indirect assays have claimed to quantify phytate (InsP(6)) levels in human biofluids, but these have been based on the initial assumption that InsP(6) is there, an assumption that our more direct assays disprove. We have shown that InsP(6) does not and cannot (because of the presence of an active In...

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Detalles Bibliográficos
Autores principales: Irvine, Robin F., Bulley, Simon J., Wilson, Miranda S., Saiardi, Adolfo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2015
Materias:
Comments and Invited Replies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4680572/
https://www.ncbi.nlm.nih.gov/pubmed/26581573
http://dx.doi.org/10.1098/rsob.150181
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author Irvine, Robin F.
Bulley, Simon J.
Wilson, Miranda S.
Saiardi, Adolfo
author_facet Irvine, Robin F.
Bulley, Simon J.
Wilson, Miranda S.
Saiardi, Adolfo
author_sort Irvine, Robin F.
collection PubMed
description Indirect assays have claimed to quantify phytate (InsP(6)) levels in human biofluids, but these have been based on the initial assumption that InsP(6) is there, an assumption that our more direct assays disprove. We have shown that InsP(6) does not and cannot (because of the presence of an active InsP(6) phosphatase in serum) exist in mammalian serum or urine. Therefore, any physiological effects of dietary InsP(6) can only be due either to its actions in the gut as a polyvalent cation chelator, or to inositol generated by its dephosphorylation by gut microflora.
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spelling pubmed-46805722015-12-29 There is no ‘Conundrum’ of InsP(6) Irvine, Robin F. Bulley, Simon J. Wilson, Miranda S. Saiardi, Adolfo Open Biol Comments and Invited Replies Indirect assays have claimed to quantify phytate (InsP(6)) levels in human biofluids, but these have been based on the initial assumption that InsP(6) is there, an assumption that our more direct assays disprove. We have shown that InsP(6) does not and cannot (because of the presence of an active InsP(6) phosphatase in serum) exist in mammalian serum or urine. Therefore, any physiological effects of dietary InsP(6) can only be due either to its actions in the gut as a polyvalent cation chelator, or to inositol generated by its dephosphorylation by gut microflora. The Royal Society 2015-11-18 /pmc/articles/PMC4680572/ /pubmed/26581573 http://dx.doi.org/10.1098/rsob.150181 Text en © 2015 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Comments and Invited Replies
Irvine, Robin F.
Bulley, Simon J.
Wilson, Miranda S.
Saiardi, Adolfo
There is no ‘Conundrum’ of InsP(6)
title There is no ‘Conundrum’ of InsP(6)
title_full There is no ‘Conundrum’ of InsP(6)
title_fullStr There is no ‘Conundrum’ of InsP(6)
title_full_unstemmed There is no ‘Conundrum’ of InsP(6)
title_short There is no ‘Conundrum’ of InsP(6)
title_sort there is no ‘conundrum’ of insp(6)
topic Comments and Invited Replies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4680572/
https://www.ncbi.nlm.nih.gov/pubmed/26581573
http://dx.doi.org/10.1098/rsob.150181
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