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Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination

Lymphocyte proliferation, mobility and longevity make them prime targets for virus infection. Myeloid cells that process and present environmental antigens to lymphocytes are consequently an important line of defence. Subcapsular sinus macrophages (SSMs) filter the afferent lymph and communicate wit...

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Autores principales: Frederico, Bruno, Chao, Brittany, Lawler, Clara, May, Janet S., Stevenson, Philip G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681069/
https://www.ncbi.nlm.nih.gov/pubmed/25872742
http://dx.doi.org/10.1099/vir.0.000140
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author Frederico, Bruno
Chao, Brittany
Lawler, Clara
May, Janet S.
Stevenson, Philip G.
author_facet Frederico, Bruno
Chao, Brittany
Lawler, Clara
May, Janet S.
Stevenson, Philip G.
author_sort Frederico, Bruno
collection PubMed
description Lymphocyte proliferation, mobility and longevity make them prime targets for virus infection. Myeloid cells that process and present environmental antigens to lymphocytes are consequently an important line of defence. Subcapsular sinus macrophages (SSMs) filter the afferent lymph and communicate with B-cells. How they interact with B-cell-tropic viruses is unknown. We analysed their encounter with murid herpesvirus-4 (MuHV-4), an experimentally accessible gammaherpesvirus related to Kaposi's sarcoma-associated herpesvirus. MuHV-4 disseminated via lymph nodes, and intranasally or subcutaneously inoculated virions readily infected SSMs. However, this infection was poorly productive. SSM depletion with clodronate-loaded liposomes or with diphtheria toxin in CD169–diphtheria toxin receptor transgenic mice increased B-cell infection and hastened virus spread to the spleen. Dendritic cells provided the main route to B-cells, and SSMs slowed host colonization, apparently by absorbing virions non-productively from the afferent lymph.
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spelling pubmed-46810692015-12-18 Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination Frederico, Bruno Chao, Brittany Lawler, Clara May, Janet S. Stevenson, Philip G. J Gen Virol Standard Lymphocyte proliferation, mobility and longevity make them prime targets for virus infection. Myeloid cells that process and present environmental antigens to lymphocytes are consequently an important line of defence. Subcapsular sinus macrophages (SSMs) filter the afferent lymph and communicate with B-cells. How they interact with B-cell-tropic viruses is unknown. We analysed their encounter with murid herpesvirus-4 (MuHV-4), an experimentally accessible gammaherpesvirus related to Kaposi's sarcoma-associated herpesvirus. MuHV-4 disseminated via lymph nodes, and intranasally or subcutaneously inoculated virions readily infected SSMs. However, this infection was poorly productive. SSM depletion with clodronate-loaded liposomes or with diphtheria toxin in CD169–diphtheria toxin receptor transgenic mice increased B-cell infection and hastened virus spread to the spleen. Dendritic cells provided the main route to B-cells, and SSMs slowed host colonization, apparently by absorbing virions non-productively from the afferent lymph. Society for General Microbiology 2015-08 /pmc/articles/PMC4681069/ /pubmed/25872742 http://dx.doi.org/10.1099/vir.0.000140 Text en © 2015 The Authors http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/(.
spellingShingle Standard
Frederico, Bruno
Chao, Brittany
Lawler, Clara
May, Janet S.
Stevenson, Philip G.
Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
title Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
title_full Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
title_fullStr Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
title_full_unstemmed Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
title_short Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
title_sort subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
topic Standard
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681069/
https://www.ncbi.nlm.nih.gov/pubmed/25872742
http://dx.doi.org/10.1099/vir.0.000140
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