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Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination
Lymphocyte proliferation, mobility and longevity make them prime targets for virus infection. Myeloid cells that process and present environmental antigens to lymphocytes are consequently an important line of defence. Subcapsular sinus macrophages (SSMs) filter the afferent lymph and communicate wit...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for General Microbiology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681069/ https://www.ncbi.nlm.nih.gov/pubmed/25872742 http://dx.doi.org/10.1099/vir.0.000140 |
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author | Frederico, Bruno Chao, Brittany Lawler, Clara May, Janet S. Stevenson, Philip G. |
author_facet | Frederico, Bruno Chao, Brittany Lawler, Clara May, Janet S. Stevenson, Philip G. |
author_sort | Frederico, Bruno |
collection | PubMed |
description | Lymphocyte proliferation, mobility and longevity make them prime targets for virus infection. Myeloid cells that process and present environmental antigens to lymphocytes are consequently an important line of defence. Subcapsular sinus macrophages (SSMs) filter the afferent lymph and communicate with B-cells. How they interact with B-cell-tropic viruses is unknown. We analysed their encounter with murid herpesvirus-4 (MuHV-4), an experimentally accessible gammaherpesvirus related to Kaposi's sarcoma-associated herpesvirus. MuHV-4 disseminated via lymph nodes, and intranasally or subcutaneously inoculated virions readily infected SSMs. However, this infection was poorly productive. SSM depletion with clodronate-loaded liposomes or with diphtheria toxin in CD169–diphtheria toxin receptor transgenic mice increased B-cell infection and hastened virus spread to the spleen. Dendritic cells provided the main route to B-cells, and SSMs slowed host colonization, apparently by absorbing virions non-productively from the afferent lymph. |
format | Online Article Text |
id | pubmed-4681069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Society for General Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-46810692015-12-18 Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination Frederico, Bruno Chao, Brittany Lawler, Clara May, Janet S. Stevenson, Philip G. J Gen Virol Standard Lymphocyte proliferation, mobility and longevity make them prime targets for virus infection. Myeloid cells that process and present environmental antigens to lymphocytes are consequently an important line of defence. Subcapsular sinus macrophages (SSMs) filter the afferent lymph and communicate with B-cells. How they interact with B-cell-tropic viruses is unknown. We analysed their encounter with murid herpesvirus-4 (MuHV-4), an experimentally accessible gammaherpesvirus related to Kaposi's sarcoma-associated herpesvirus. MuHV-4 disseminated via lymph nodes, and intranasally or subcutaneously inoculated virions readily infected SSMs. However, this infection was poorly productive. SSM depletion with clodronate-loaded liposomes or with diphtheria toxin in CD169–diphtheria toxin receptor transgenic mice increased B-cell infection and hastened virus spread to the spleen. Dendritic cells provided the main route to B-cells, and SSMs slowed host colonization, apparently by absorbing virions non-productively from the afferent lymph. Society for General Microbiology 2015-08 /pmc/articles/PMC4681069/ /pubmed/25872742 http://dx.doi.org/10.1099/vir.0.000140 Text en © 2015 The Authors http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/(. |
spellingShingle | Standard Frederico, Bruno Chao, Brittany Lawler, Clara May, Janet S. Stevenson, Philip G. Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination |
title | Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination |
title_full | Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination |
title_fullStr | Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination |
title_full_unstemmed | Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination |
title_short | Subcapsular sinus macrophages limit acute gammaherpesvirus dissemination |
title_sort | subcapsular sinus macrophages limit acute gammaherpesvirus dissemination |
topic | Standard |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681069/ https://www.ncbi.nlm.nih.gov/pubmed/25872742 http://dx.doi.org/10.1099/vir.0.000140 |
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