P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design

Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS am...

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Autores principales: Fahlgren, Noah, Hill, Steven T., Carrington, James C., Carbonell, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Applications Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681993/
https://www.ncbi.nlm.nih.gov/pubmed/26382195
http://dx.doi.org/10.1093/bioinformatics/btv534
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author Fahlgren, Noah
Hill, Steven T.
Carrington, James C.
Carbonell, Alberto
author_facet Fahlgren, Noah
Hill, Steven T.
Carrington, James C.
Carbonell, Alberto
author_sort Fahlgren, Noah
collection PubMed
description Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into ‘B/c’ compatible vectors. Availability and implementation: The P-SAMS website is available at http://p-sams.carringtonlab.org. Contact: acarbonell@ibmcp.upv.es or nfahlgren@danforthcenter.org
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spelling pubmed-46819932015-12-18 P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design Fahlgren, Noah Hill, Steven T. Carrington, James C. Carbonell, Alberto Bioinformatics Applications Notes Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into ‘B/c’ compatible vectors. Availability and implementation: The P-SAMS website is available at http://p-sams.carringtonlab.org. Contact: acarbonell@ibmcp.upv.es or nfahlgren@danforthcenter.org Oxford University Press 2016-01-01 2015-09-17 /pmc/articles/PMC4681993/ /pubmed/26382195 http://dx.doi.org/10.1093/bioinformatics/btv534 Text en © The Author 2015. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Applications Notes
Fahlgren, Noah
Hill, Steven T.
Carrington, James C.
Carbonell, Alberto
P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design
title P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design
title_full P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design
title_fullStr P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design
title_full_unstemmed P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design
title_short P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design
title_sort p-sams: a web site for plant artificial microrna and synthetic trans-acting small interfering rna design
topic Applications Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681993/
https://www.ncbi.nlm.nih.gov/pubmed/26382195
http://dx.doi.org/10.1093/bioinformatics/btv534
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