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Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection

Blast is the most common biotic stress leading to the reduction of rice yield in many rice-growing areas of the world, including Malaysia. Improvement of blast resistance of rice varieties cultivated in blast endemic areas is one of the most important objectives of rice breeding programs. In this st...

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Autores principales: Tanweer, Fatah A., Rafii, Mohd Y., Sijam, Kamaruzaman, Rahim, Harun A., Ahmed, Fahim, Ashkani, Sadegh, Latif, Mohammad A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682138/
https://www.ncbi.nlm.nih.gov/pubmed/26734013
http://dx.doi.org/10.3389/fpls.2015.01002
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author Tanweer, Fatah A.
Rafii, Mohd Y.
Sijam, Kamaruzaman
Rahim, Harun A.
Ahmed, Fahim
Ashkani, Sadegh
Latif, Mohammad A.
author_facet Tanweer, Fatah A.
Rafii, Mohd Y.
Sijam, Kamaruzaman
Rahim, Harun A.
Ahmed, Fahim
Ashkani, Sadegh
Latif, Mohammad A.
author_sort Tanweer, Fatah A.
collection PubMed
description Blast is the most common biotic stress leading to the reduction of rice yield in many rice-growing areas of the world, including Malaysia. Improvement of blast resistance of rice varieties cultivated in blast endemic areas is one of the most important objectives of rice breeding programs. In this study, the marker-assisted backcrossing strategy was applied to improve the blast resistance of the most popular Malaysian rice variety MR219 by introgressing blast resistance genes from the Pongsu Seribu 2 variety. Two blast resistance genes, Pi-b and Pi-kh, were pyramided into MR219. Foreground selection coupled with stringent phenotypic selection identified 15 plants homozygous for the Pi-b and Pi-kh genes, and background selection revealed more than 95% genome recovery of MR219 in advanced blast resistant lines. Phenotypic screening against blast disease indicated that advanced homozygous blast resistant lines were strongly resistant against pathotype P7.2 in the blast disease endemic areas. The morphological, yield, grain quality, and yield-contributing characteristics were significantly similar to those of MR219. The newly developed blast resistant improved lines will retain the high adoptability of MR219 by farmers. The present results will also play an important role in sustaining the rice production of Malaysia.
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spelling pubmed-46821382016-01-05 Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection Tanweer, Fatah A. Rafii, Mohd Y. Sijam, Kamaruzaman Rahim, Harun A. Ahmed, Fahim Ashkani, Sadegh Latif, Mohammad A. Front Plant Sci Plant Science Blast is the most common biotic stress leading to the reduction of rice yield in many rice-growing areas of the world, including Malaysia. Improvement of blast resistance of rice varieties cultivated in blast endemic areas is one of the most important objectives of rice breeding programs. In this study, the marker-assisted backcrossing strategy was applied to improve the blast resistance of the most popular Malaysian rice variety MR219 by introgressing blast resistance genes from the Pongsu Seribu 2 variety. Two blast resistance genes, Pi-b and Pi-kh, were pyramided into MR219. Foreground selection coupled with stringent phenotypic selection identified 15 plants homozygous for the Pi-b and Pi-kh genes, and background selection revealed more than 95% genome recovery of MR219 in advanced blast resistant lines. Phenotypic screening against blast disease indicated that advanced homozygous blast resistant lines were strongly resistant against pathotype P7.2 in the blast disease endemic areas. The morphological, yield, grain quality, and yield-contributing characteristics were significantly similar to those of MR219. The newly developed blast resistant improved lines will retain the high adoptability of MR219 by farmers. The present results will also play an important role in sustaining the rice production of Malaysia. Frontiers Media S.A. 2015-12-17 /pmc/articles/PMC4682138/ /pubmed/26734013 http://dx.doi.org/10.3389/fpls.2015.01002 Text en Copyright © 2015 Tanweer, Rafii, Sijam, Rahim, Ahmed, Ashkani and Latif. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Tanweer, Fatah A.
Rafii, Mohd Y.
Sijam, Kamaruzaman
Rahim, Harun A.
Ahmed, Fahim
Ashkani, Sadegh
Latif, Mohammad A.
Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection
title Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection
title_full Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection
title_fullStr Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection
title_full_unstemmed Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection
title_short Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection
title_sort introgression of blast resistance genes (putative pi-b and pi-kh) into elite rice cultivar mr219 through marker-assisted selection
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682138/
https://www.ncbi.nlm.nih.gov/pubmed/26734013
http://dx.doi.org/10.3389/fpls.2015.01002
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