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Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling

To understand the physiological and molecular mechanisms underlying seedling salt tolerance in rice (Oryza sativa L.), the phenotypic, metabolic, and transcriptome responses of two related rice genotypes, IR64 and PL177, with contrasting salt tolerance were characterized under salt stress and salt+a...

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Autores principales: Wang, Wen-Sheng, Zhao, Xiu-Qin, Li, Min, Huang, Li-Yu, Xu, Jian-Long, Zhang, Fan, Cui, Yan-Ru, Fu, Bin-Ying, Li, Zhi-Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682442/
https://www.ncbi.nlm.nih.gov/pubmed/26512058
http://dx.doi.org/10.1093/jxb/erv476
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author Wang, Wen-Sheng
Zhao, Xiu-Qin
Li, Min
Huang, Li-Yu
Xu, Jian-Long
Zhang, Fan
Cui, Yan-Ru
Fu, Bin-Ying
Li, Zhi-Kang
author_facet Wang, Wen-Sheng
Zhao, Xiu-Qin
Li, Min
Huang, Li-Yu
Xu, Jian-Long
Zhang, Fan
Cui, Yan-Ru
Fu, Bin-Ying
Li, Zhi-Kang
author_sort Wang, Wen-Sheng
collection PubMed
description To understand the physiological and molecular mechanisms underlying seedling salt tolerance in rice (Oryza sativa L.), the phenotypic, metabolic, and transcriptome responses of two related rice genotypes, IR64 and PL177, with contrasting salt tolerance were characterized under salt stress and salt+abscisic acid (ABA) conditions. PL177 showed significantly less salt damage, lower Na(+)/K(+) ratios in shoots, and Na(+) translocation from roots to shoots, attributed largely to better salt exclusion from its roots and salt compartmentation of its shoots. Exogenous ABA was able to enhance the salt tolerance of IR64 by selectively decreasing accumulation of Na(+) in its roots and increasing K(+) in its shoots. Salt stress induced general and organ-specific increases of many primary metabolites in both rice genotypes, with strong accumulation of several sugars plus proline in shoots and allantoin in roots. This was due primarily to ABA-mediated repression of genes for degradation of these metabolites under salt. In PL177, salt specifically up-regulated genes involved in several pathways underlying salt tolerance, including ABA-mediated cellular lipid and fatty acid metabolic processes and cytoplasmic transport, sequestration by vacuoles, detoxification and cell-wall remodeling in shoots, and oxidation–reduction reactions in roots. Combined genetic and transcriptomic evidence shortlisted relatively few candidate genes for improved salt tolerance in PL177.
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spelling pubmed-46824422015-12-18 Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling Wang, Wen-Sheng Zhao, Xiu-Qin Li, Min Huang, Li-Yu Xu, Jian-Long Zhang, Fan Cui, Yan-Ru Fu, Bin-Ying Li, Zhi-Kang J Exp Bot Research Paper To understand the physiological and molecular mechanisms underlying seedling salt tolerance in rice (Oryza sativa L.), the phenotypic, metabolic, and transcriptome responses of two related rice genotypes, IR64 and PL177, with contrasting salt tolerance were characterized under salt stress and salt+abscisic acid (ABA) conditions. PL177 showed significantly less salt damage, lower Na(+)/K(+) ratios in shoots, and Na(+) translocation from roots to shoots, attributed largely to better salt exclusion from its roots and salt compartmentation of its shoots. Exogenous ABA was able to enhance the salt tolerance of IR64 by selectively decreasing accumulation of Na(+) in its roots and increasing K(+) in its shoots. Salt stress induced general and organ-specific increases of many primary metabolites in both rice genotypes, with strong accumulation of several sugars plus proline in shoots and allantoin in roots. This was due primarily to ABA-mediated repression of genes for degradation of these metabolites under salt. In PL177, salt specifically up-regulated genes involved in several pathways underlying salt tolerance, including ABA-mediated cellular lipid and fatty acid metabolic processes and cytoplasmic transport, sequestration by vacuoles, detoxification and cell-wall remodeling in shoots, and oxidation–reduction reactions in roots. Combined genetic and transcriptomic evidence shortlisted relatively few candidate genes for improved salt tolerance in PL177. Oxford University Press 2016-01 2015-10-27 /pmc/articles/PMC4682442/ /pubmed/26512058 http://dx.doi.org/10.1093/jxb/erv476 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Wang, Wen-Sheng
Zhao, Xiu-Qin
Li, Min
Huang, Li-Yu
Xu, Jian-Long
Zhang, Fan
Cui, Yan-Ru
Fu, Bin-Ying
Li, Zhi-Kang
Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
title Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
title_full Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
title_fullStr Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
title_full_unstemmed Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
title_short Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
title_sort complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682442/
https://www.ncbi.nlm.nih.gov/pubmed/26512058
http://dx.doi.org/10.1093/jxb/erv476
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